Hexamethonium (Bromide)
Based on 5 publication(s) in Google Scholar
Hexamethonium Bromide is a non-selective ganglionic nicotinic-receptor antagonist (nAChR) antagonist, with mixed competitive and noncompetitive activity. Hexamethonium Bromide has anti-hypertensive activity. Hexamethonium Bromide attenuates sympathetic activity and blood pressure in spontaneously hypertensive animal models.
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- 純度: 99.75%
- CAS 番号: 55-97-0
- 分子式: C12H30Br2N2
- 分子量:362.19
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保管条件:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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MedChemExpress（MCE）の使用を引用している文献 Hexamethonium Bromide
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生物活性
nAChR[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| J774.1 | IC50 |
>100 μM
Compound: 3d
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Cytotoxicity against mouse J774.1 cells after 48 hrs by alamar blue satining
Cytotoxicity against mouse J774.1 cells after 48 hrs by alamar blue satining
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[PMID: 20382538] |
Hexamethonium Bromide (100 μM; 60 minutes) abolishes epibatidine-induced depolarisations of smooth muscle cells[1].
The α3β4α5 receptors have greater sensitivity to Hexamethonium Bromide than the other potential ganglionic models, α3β4, α3β2, and α3β2α5[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Hexamethonium Bromide (0.2-1.0 mg/kg; i.v.) treatments show no significant differences in the RSNA, MAP or HR between Wistar rats and SHRs[4].
Hexamethonium Bromide (5.0-25 mg/kg; i.v.) results in a greater reduction in the RSNA and MAP in SHRs compared with Wistar rats[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male normotensive Wistar rats (280-320 g), SHRs[4]
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Dosage:0.2 mg/kg, 1.0 mg/kg, 5.0 mg/kg, 25 mg/kg
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Administration:Intravenous injection
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Result:Significantly reduced the RSNA, MAP and HR in the Wistar rats and the SHRs.
化学情報
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CAS 番号 55-97-0
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性状 Solid
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分子量 362.19
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分子式 C12H30Br2N2
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Color White to off-white
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SMILES
C[N+](C)(C)CCCCCC[N+](C)(C)C.[Br-].[Br-]
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別名
臭化ヘキサメトニウム
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (5)
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Journal Impact Factor
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Most Recent
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Autophagy
E-cigarette aerosols induce the hydrolysis of lysosomal glycerophospholipids through PLA2G4A activation initiated by nicotine binding to CHRNA3/α3 nAChR in airway epithelial cells. [Abstract]2026 Jun 21:1-25. PMID: 42287088 -
Atherosclerosis
Nicotine-mediated autophagy of vascular smooth muscle cell accelerates atherosclerosis via nAChRs/ROS/NF-κB signaling pathway. [Abstract]2019 May:284:1-10. PMID: 30856513 -
Am J Physiol Renal Physiol
Anatomic and functional evidence for renal autonomic innervation in normotensive and hypertensive rats. [Abstract]2024 Nov 1;327(5):F885-F898. PMID: 39298550 -
Eur J Neurosci
Chemerin in caudal division of nucleus tractus solitarius increases sympathetic activity and blood pressure. [Abstract]2024 Sep;60(5):4830-4842. PMID: 39044301 -
Evid Based Complement Alternat Med
The Inhibition Effects of Shenmai Injection on Acetylcholine-Induced Catecholamine Synthesis and Secretion by Modulating Nicotinic Acetylcholine Receptor Ion Channels in Cultured Bovine Adrenal Medullary Cells. [Abstract]2020 Dec 15:2020:8514926. PMID: 33456492
溶剤 & 溶解度
H2O : ≥ 100 mg/mL (276.10 mM)
DMSO : 16.67 mg/mL (46.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (4.61 mM); Clear solution
This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (4.61 mM); Clear solution
This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (276.10 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
純度とドキュメンテーション
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データシート (273 KB)
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SDS (420 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Damian J. Williams, et al. Mechanisms involved in nicotinic acetylcholine receptor-induced neurotransmitter release from sympathetic nerve terminals in the mouse vas deferens. PLoS One. 2011; 6(12): e29209. [Content Brief]
[2]. Roger L. Papke, et al. Activation and Inhibition of Mouse Muscle and Neuronal Nicotinic Acetylcholine Receptors Expressed in Xenopus Oocytes. J Pharmacol Exp Ther. 2010 May; 333(2): 501–518. [Content Brief]
[3]. Brian T Hawkins, et al. Modulation of cerebral microvascular permeability by endothelial nicotinic acetylcholine receptors. Am J Physiol Heart Circ Physiol. 2005 Jul;289(1):H212-9. [Content Brief]
[4]. Peng Li, et al. Hexamethonium attenuates sympathetic activity and blood pressure in spontaneously hypertensive rats. Mol Med Rep. 2015 Nov;12(5):7116-22. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.7610 mL | 13.8049 mL | 27.6098 mL | 69.0245 mL |
| 5 mM | 0.5522 mL | 2.7610 mL | 5.5220 mL | 13.8049 mL | |
| 10 mM | 0.2761 mL | 1.3805 mL | 2.7610 mL | 6.9025 mL | |
| 15 mM | 0.1841 mL | 0.9203 mL | 1.8407 mL | 4.6016 mL | |
| 20 mM | 0.1380 mL | 0.6902 mL | 1.3805 mL | 3.4512 mL | |
| 25 mM | 0.1104 mL | 0.5522 mL | 1.1044 mL | 2.7610 mL | |
| 30 mM | 0.0920 mL | 0.4602 mL | 0.9203 mL | 2.3008 mL | |
| 40 mM | 0.0690 mL | 0.3451 mL | 0.6902 mL | 1.7256 mL | |
| H2O | 50 mM | 0.0552 mL | 0.2761 mL | 0.5522 mL | 1.3805 mL |
| 60 mM | 0.0460 mL | 0.2301 mL | 0.4602 mL | 1.1504 mL | |
| 80 mM | 0.0345 mL | 0.1726 mL | 0.3451 mL | 0.8628 mL | |
| 100 mM | 0.0276 mL | 0.1380 mL | 0.2761 mL | 0.6902 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Hexamethonium Bromide 関連分類
HY-B0569 関連分類
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