製品番号: HY-127009

Levoleucovorin

(別名: Levofolinic acid; Levofolene)

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Based on 1 Customer Validation

Levoleucovorin (Levofolinic acid; Levofolene) is the pharmacologically and orally active levoisomer of Folinic acid (HY-17556), a synthetic folate analogue. Levoleucovorin can inhibit LOXL2 with an IC₅₀ of 68.81 μM. Levoleucovorin can inhibit cancer cells proliferation, migration and induce apoptosis. Levoleucovorin can be used as a rescue agent for high-dose Methotrexate (HY-14519). Levoleucovorin can reduce the frequency of embryonic malformations. Levoleucovorin can be used for the research of can and endocrinology, such as breast cancer and osteosarcoma.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

純度: 98.53%
CAS 番号: 68538-85-2
分子式: C₂₀H₂₃N₇O₇
分子量:473.44

保管条件:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Levoleucovorin 構造式

CAS 番号: 68538-85-2

容量

価格（税別）

在庫状況

数量

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性
- 製品説明
- IC₅₀ & Target
- 体外実験
- 体内実験
化学情報
溶剤 & 溶解度
- 体外
純度とドキュメンテーション
参考文献
Help & FAQs
関連分類
Bioz

生物活性

製品説明

IC₅₀ & Target^[1]

LOXL2

68.81 nM (IC₅₀)

体外実験

Levoleucovorin inhibits recombinant human LOXL2 protein with an IC₅₀ of 68.81 μM^[1].
Levoleucovorin (72 h) inhibits proliferation of MDA-MB 231 breast cancer cells with a GI₅₀ of 55.91 μM and MCF-7 breast cancer cells with a GI₅₀ of 79.20 μM^[1].
Levoleucovorin (15-60 μM; 12 h) dose-dependently inhibits HGF-induced trans-endothelial migration of MDA-MB 231 and MCF-7 breast cancer cells across HUVEC cell layers^[1].
Levoleucovorin (30-80 μM；72 h) induces dose-dependent apoptosis in MDA-MB 231 and MCF-7 breast cancer cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

MDA-MB 231 breast cancer cell line, MCF-7 breast cancer cell line
Concentration:

30 μM, 60 μM (MDA-MB 231); 40 μM, 80 μM (MCF-7)
Incubation Time:

72 h
Result:

Significantly increased Annexin V-positive early and late apoptotic populations in MDA-MB 231 cells in a dose-dependent manner, with total apoptosis increasing 17.93% at 30 μM and 33.11% at 60 μM.
Significantly increased Annexin V-positive early and late apoptotic populations in MCF-7 cells in a dose-dependent manner, with total apoptosis increasing 15.36% at 40 μM and 30.38% at 80 μM.

体内実験

Levoleucovorin (10.75-43 μmol/kg per day; i.g.,; daily; initiated 2 weeks pre-mating through gestation) reduces the frequency of embryonic malformations in a dose-dependent manner, with the 43 μmol/kg per day dose lowering overall malformation frequency to 33.3%^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Folbp1 knockout (female; mated to Folbp1-/- males; prenatal developmental model)^[3]
Dosage:

10.75 μmol/kg per day; 21.5 μmol/kg per day; 43 μmol/kg per day
Administration:

I.g.; daily; initiated 2 weeks pre-mating through gestation
Result:

Reduced malformation frequency to 66.7% at 10.75 μmol/kg per day, 65.8% at 21.5 μmol/kg per day, and 33.3% at 43 μmol/kg per day.
Decreased neural tube defects to 35.2% (10.75 and 21.5 μmol/kg), 16.7% (43 μmol/kg).
Decreased craniofacial defects to 46.2% (10.75 and 21.5 μmol/kg), 19.4% (43 μmol/kg).
Demonstrated significant inverse dose-dependent relationship with all malformation categories.
Resulted in a mean litter size of 3.70 ± 2.63 at 43 μmol/kg per day, significantly lower than wild-type controls.

化学情報

CAS 番号 68538-85-2
性状 Solid
分子量 473.44
分子式 C₂₀H₂₃N₇O₇
Color Off-white to light yellow
SMILES

O=CN1C2=C(NC(N)=NC2=O)NC[C@@H]1CNC3=CC=C(C=C3)C(N[C@H](C(O)=O)CCC(O)=O)=O
別名

Levofolinic acid; Levofolene
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 35 mg/mL (73.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 33.33 mg/mL (70.40 mM; ultrasonic and adjust pH to 11 with 1 M NaOH)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1122 mL

10.5610 mL

21.1220 mL

5 mM

0.4224 mL

2.1122 mL

4.2244 mL

10 mM

0.2112 mL

1.0561 mL

2.1122 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (273 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Deshpande H, et al. Levoleucovorin inhibits LOXL2 (lysyl oxidase like-2) to control breast cancer proliferation: a repurposing approach. J Biomol Struct Dyn. 2024;42(10):5104-5113. [Content Brief]

[2]. Chuang VT, et al. Levoleucovorin as replacement for leucovorin in cancer treatment. Ann Pharmacother. 2012;46(10):1349-1357. [Content Brief]

[3]. Spiegelstein O, et al. Embryonic development of folate binding protein-1 (Folbp1) knockout mice: Effects of the chemical form, dose, and timing of maternal folate supplementation. Dev Dyn. 2004;231(1):221-231. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.1122 mL	10.5610 mL	21.1220 mL	52.8050 mL
	5 mM	0.4224 mL	2.1122 mL	4.2244 mL	10.5610 mL
	10 mM	0.2112 mL	1.0561 mL	2.1122 mL	5.2805 mL
	15 mM	0.1408 mL	0.7041 mL	1.4081 mL	3.5203 mL
	20 mM	0.1056 mL	0.5281 mL	1.0561 mL	2.6403 mL
	25 mM	0.0845 mL	0.4224 mL	0.8449 mL	2.1122 mL
	30 mM	0.0704 mL	0.3520 mL	0.7041 mL	1.7602 mL
	40 mM	0.0528 mL	0.2640 mL	0.5281 mL	1.3201 mL
	50 mM	0.0422 mL	0.2112 mL	0.4224 mL	1.0561 mL
	60 mM	0.0352 mL	0.1760 mL	0.3520 mL	0.8801 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Levoleucovorin

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生物活性

化学情報

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

Levoleucovorin 関連分類

HY-127009 関連分類