Phos-FLAG
Phos-FLAG is a phosphine probe containing the Flag peptide. Phos-FLAG undergoes Staudinger ligation with azido groups metabolically incorporated into cell surface glycans and organ glycoproteins, enabling quantitative analysis of the presence of cell surface azides. Phos-FLAG detects azide-labeled glycans in isolated cells and organ lysates.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 403481-00-5
- 分子式: C62H75N10O23P
- 分子量:1359.29
-
保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
生物活性
製品説明
体内実験
Phos-FLAG (16 μM; i.p.; single dose; 24 h after final Ac4ManNAz injection) successfully mediates Staudinger ligation with metabolically introduced azide groups on mouse splenocytes in vivo, generating detectable cell-surface Flag epitopes with no observed toxicity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Es1^e/Es1^e mice (plasma esterase-deficient, male and female); B6D2F1 mice (wild-type, male and female)[1]
-
Dosage:250 μM (ex vivo incubation for 1 h); 500 μM (ex vivo incubation for 6-12 h)
-
Administration:ex vivo incubation; 1 h (250 μM); 6-12 h (500 μM); room temperature
-
Result:Exhibited a dose-dependent increase in mean fluorescence intensity (MFI) in splenocytes from Ac4ManNAz-treated mice, with MFI values significantly higher than vehicle-treated controls.
Detected discrete labelled glycoprotein bands in heart, kidney, and liver lysates from Ac4ManNAz-treated mice, but not in brain or thymus homogenates.
Significantly reduced MFI after Phos-Flag labelling in sialidase-treated splenocytes from Ac4ManNAz-treated mice, confirming azide label presence on sialic acids.
-
Animal Model:C57BL/6 mice[1]
-
Dosage:16 μM
-
Administration:i.p.; single dose; 24 hours after final Ac4ManNAz injection
-
Result:Showed a significant increase in MFI in splenocytes from mice treated with both Ac4ManNAz and Phos-Flag relative to untreated mice or mice treated with only one agent.
Produced a larger MFI increase in ex vivo Phos-Flag treatment of splenocytes from mice given only Ac4ManNAz compared to ex vivo treatment of splenocytes from mice given both Ac4ManNAz and in vivo Phos-Flag, indicating significant in vivo Staudinger ligation of cell-surface azides.
Observed no adverse physiological effects in mice after administration.
化学情報
-
CAS 番号 403481-00-5
-
分子量 1359.29
-
分子式 C62H75N10O23P
-
SMILES
O=C(OC)C(C=C1)=C(P(C2=CC=CC=C2)C3=CC=CC=C3)C=C1C(N[C@@H](CC(O)=O)C(N[C@@H](CC4=CC=C(C=C4)O)C(N[C@@H](CCCCN)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CCCCN)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Phos-FLAG 関連分類
HY-P11710 関連分類
Powered by Bioz