Phos-FLAG
Based on 1 Customer Validation
Phos-FLAG is a phosphine probe containing the Flag peptide. Phos-FLAG undergoes Staudinger ligation with azido groups metabolically incorporated into cell surface glycans and organ glycoproteins, enabling quantitative analysis of the presence of cell surface azides. Phos-FLAG detects azide-labeled glycans in isolated cells and organ lysates.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 95.30%
- CAS 番号: 403481-00-5
- 分子式: C62H75N10O23P
- 分子量:1359.29
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体内実験
Phos-FLAG (16 μM; i.p.; single dose; 24 h after final Ac4ManNAz injection) successfully mediates Staudinger ligation with metabolically introduced azide groups on mouse splenocytes in vivo, generating detectable cell-surface Flag epitopes with no observed toxicity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Es1^e/Es1^e mice (plasma esterase-deficient, male and female); B6D2F1 mice (wild-type, male and female)[1]
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Dosage:250 μM (ex vivo incubation for 1 h); 500 μM (ex vivo incubation for 6-12 h)
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Administration:ex vivo incubation; 1 h (250 μM); 6-12 h (500 μM); room temperature
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Result:Exhibited a dose-dependent increase in mean fluorescence intensity (MFI) in splenocytes from Ac4ManNAz-treated mice, with MFI values significantly higher than vehicle-treated controls.
Detected discrete labelled glycoprotein bands in heart, kidney, and liver lysates from Ac4ManNAz-treated mice, but not in brain or thymus homogenates.
Significantly reduced MFI after Phos-Flag labelling in sialidase-treated splenocytes from Ac4ManNAz-treated mice, confirming azide label presence on sialic acids.
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Animal Model:C57BL/6 mice[1]
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Dosage:16 μM
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Administration:i.p.; single dose; 24 hours after final Ac4ManNAz injection
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Result:Showed a significant increase in MFI in splenocytes from mice treated with both Ac4ManNAz and Phos-Flag relative to untreated mice or mice treated with only one agent.
Produced a larger MFI increase in ex vivo Phos-Flag treatment of splenocytes from mice given only Ac4ManNAz compared to ex vivo treatment of splenocytes from mice given both Ac4ManNAz and in vivo Phos-Flag, indicating significant in vivo Staudinger ligation of cell-surface azides.
Observed no adverse physiological effects in mice after administration.
化学情報
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CAS 番号 403481-00-5
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性状 Solid
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分子量 1359.29
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分子式 C62H75N10O23P
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Color White to off-white
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SMILES
O=C(OC)C(C=C1)=C(P(C2=CC=CC=C2)C3=CC=CC=C3)C=C1C(N[C@@H](CC(O)=O)C(N[C@@H](CC4=CC=C(C=C4)O)C(N[C@@H](CCCCN)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CCCCN)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外:
DMSO : 20 mg/mL (14.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
純度とドキュメンテーション
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データシート (284 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
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- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7357 mL | 3.6784 mL | 7.3568 mL | 18.3920 mL |
| 5 mM | 0.1471 mL | 0.7357 mL | 1.4714 mL | 3.6784 mL | |
| 10 mM | 0.0736 mL | 0.3678 mL | 0.7357 mL | 1.8392 mL |
Phos-FLAG 関連分類
HY-P11710 関連分類
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