PYSNO
PYSNO is a lysosome-targeted fluorescent probe based on a pyridazinone skeleton (λem=515-565 nm, λex=405 nm) that can be used to track nitric oxide (NO) production in vivo. PYSNO exhibits a rapid, highly sensitive and highly selective "turn-on" response to endogenous and exogenous NO by blocking photoinduced electron transfer and regulating radiative decay rates. PYSNO enables precise in vivo monitoring in a mouse model of myocardial fibrosis and can be applied to the research of related diseases.
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- CAS 番号: 2445485-84-5
- 分子式: C28H31N5O3S
- 分子量:517.64
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
In cell-free fluorescence spectroscopy assays, PYSNO (5 μM; 10 min, 60 s, 15 s) rapidly detects NO with a 23-fold fluorescence enhancement, a linear response range of 1 to 30 μM, and a limit of detection of 242 nM[1].
PYSNO (5 μM; 30 min) selectively accumulates in lysosomes of SH-SY5Y human neuroblastoma cells, as confirmed by a Pearson’s colocalization coefficient of 0.87 with Lyso-Tracker Red (HY-D1300) after NO-induced fluorescence activation[1].
PYSNO (5 μM; 30 min) detects exogenous NO in SH-SY5Y human neuroblastoma cells with dose-dependent fluorescence activation, showing a 10.5-fold enhancement at 200 μM NO[1].
PYSNO (5 μM; 30 min) detects endogenously produced NO in RAW264.7 mouse macrophages, showing a 2.8-fold fluorescence enhancement after NO induction, with signal reduction by NOS inhibition or NO scavenging[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 mice (male, 10 weeks old; myocardial fibrosis induced by subcutaneous isoproterenol injection)[1]
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Dosage:50 μM (200 μL, 0.2% DMSO in PBS)
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Administration:i.v. (tail vein); single dose
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Result:Showed highly enhanced fluorescence in the heart region of myocardial fibrosis model mice relative to normal mice in in vivo imaging.
Confirmed significantly higher fluorescence intensity in myocardial fibrosis model mice relative to normal mice in ex vivo imaging of harvested hearts.
化学情報
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CAS 番号 2445485-84-5
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分子量 517.64
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分子式 C28H31N5O3S
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SMILES
NC1=C(C=C(C=C1)C2=CC=C(S2)N3N=C(C=CC3=O)C4=CC=C(C=C4)OCCCCN5CCOCC5)N
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
[1]. Zhou T, et al. A Smart Fluorescent Probe for NO Detection and Application in Myocardial Fibrosis Imaging. Anal Chem. 2020;92(7):5064-5072. [Content Brief]
[2]. Dong X, et al. Theoretical insights into the linker effects on the turn-on fluorescence behaviors in pyridazinone-containing NO probes. Spectrochim Acta A Mol Biomol Spectrosc. 2024;321:124761. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
PYSNO 関連分類
HY-D3250 関連分類
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