Thanatin
Based on 1 Customer Validation
Thanatin is an inducible cationic antimicrobial peptide. Thanatin is a pathogen-inducible single-disulfide-bond-containing β-hairpin AMP. Thanatin displays broad-spectrum activity against both Gram-negative and Gram-positive bacteria as well as against various species of fungi with MICs of 0.3-40 µM, 0.6-40 µM and 0.6-20 µM, respectively. Thanatin has the property of competitive replacement of divalent cations from bacterial outer membrane (OM), leading to OM disruption.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.47%
- CAS 番号: 214542-43-5
- 分子式: C103H177N35O27S3
- 分子量:2433.92
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Thanatin is strongly cationic (pI of 10.48) and contains a distinct short eight-residue basic loop created through a disulfide bond formation between residues Cys11 and Cys18 at the C-terminus. [1].
Thanatin exhibits potent inhibitory effect on the growth of all New Delhi metallo-β-lactamase-1 (NDM-1)-producing E. coli and K. pneumoniae strains at 0.4-3.2 μM of the minimum inhibitory concentration (MIC) values[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male BALB/c mice aged 8-10 weeks and weighing 18-22 g with CFU E. coli XJ141026
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Dosage:1, 3, 6 mg/kg
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Administration:Intraperitoneally injected; at 1 and 6 h
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Result:1, 3, and 6 mg/kg markedly increased the survival rate from 0 to 30%, 70%, and 100%, respectively.
Showed that the bacterial titers decreased with increasing drug dose.
Rescued the pathological damages (large amounts of inflammatory cell infiltration, alveolar fusion, congestion in the spleen red pulp area, hepatic sinusoidal dilation and congestion) in a dose-dependent manner.
化学情報
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CAS 番号 214542-43-5
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性状 Solid
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分子量 2433.92
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分子式 C103H177N35O27S3
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Color White to off-white
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配列
Gly-Ser-Lys-Lys-Pro-Val-Pro-Ile-Ile-Tyr-Cys-Asn-Arg-Arg-Thr-Gly-Lys-Cys-Gln-Arg-Met (Disulfide bridge:Cys11-Cys18)
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シーケンスの短縮
GSKKPVPIIYCNRRTGKCQRM (Disulfide bridge:Cys11-Cys18)
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
H2O : ≥ 100 mg/mL (41.09 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (291 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Rachita Dash, et al. Thanatin: An Emerging Host Defense Antimicrobial Peptide with Multiple Modes of Action. Int J Mol Sci. 2021 Feb 3;22(4):1522. [Content Brief]
[2]. Bo Ma, et al. The antimicrobial peptide thanatin disrupts the bacterial outer membrane and inactivates the NDM-1 metallo-β-lactamase. Nat Commun. 2019 Aug 6;10(1):3517. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.4109 mL | 2.0543 mL | 4.1086 mL | 10.2715 mL |
| 5 mM | 0.0822 mL | 0.4109 mL | 0.8217 mL | 2.0543 mL | |
| 10 mM | 0.0411 mL | 0.2054 mL | 0.4109 mL | 1.0271 mL | |
| 15 mM | 0.0274 mL | 0.1370 mL | 0.2739 mL | 0.6848 mL | |
| 20 mM | 0.0205 mL | 0.1027 mL | 0.2054 mL | 0.5136 mL | |
| 25 mM | 0.0164 mL | 0.0822 mL | 0.1643 mL | 0.4109 mL | |
| 30 mM | 0.0137 mL | 0.0685 mL | 0.1370 mL | 0.3424 mL | |
| 40 mM | 0.0103 mL | 0.0514 mL | 0.1027 mL | 0.2568 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Thanatin 関連分類
HY-P5601 関連分類
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