Direct Blue 1
Based on 3 publication(s) in Google Scholar
Direct Blue 1 (Chicago Sky Blue 6B) is a complex dye for background autofluorescence in immunofluorescence histochemistry. Direct Blue 1 is a potent and competitive VGLUT inhibitor. Direct Blue 1 can inhibit the Aβ-binding small molecule PrP ligand. Direct Blue 1 has anti-inflammatory activity.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 2610-05-1
- Formula: C34H24N6Na4O16S4
- Molecular Weight:992.80
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Direct Blue 1
More
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| AIK-T4 | EC50 |
4.8 μM
Compound: 4
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Ability to prevent HIV-1 induced T4 cell killing and HIV-1 replication.
Ability to prevent HIV-1 induced T4 cell killing and HIV-1 replication.
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[PMID: 7752188] |
Direct Blue 1 (0.1-10 μM, 9 days) can bind α-synuclein and inhibit its aggregation, thus blocking the proliferation of α-synuclein between SH-SY5Y cells[4].
Direct Blue 1 (5-20 μM, 48 h) inhibits RANKL-induced osteoclasts and bone resorption in vitro by inhibiting NF-κB signaling and promoting proteasome-mediated MIF degradation[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BMMs
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Concentration:0.75, 1.5, 3.125, 6.25, 12.5, 25, 50, 100, 200 μM
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Incubation Time:48, 96 h
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Result:Exhibited no cytotoxic effect at concentrations of 0-50 μM.
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Cell Line:BMMs
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Concentration:5, 10, 20 μM
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Incubation Time:0, 1, 3, 5 days
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Result:Attenuated the protein expression of both c-Fos and NFATc1.
Decreased the protein expression of MIF.
Direct Blue 1 (7.5 μg, intraventricular injection) alleviates methamphetamine-induced hyperactivity and behavioral sensitization in mice[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:OVX-induced osteoporosis mice model[5]
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Dosage:2, 8 m/kg
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Administration:s.c.
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Result:Inhibited the elevated osteoclast formation and bone resorption.
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Animal Model:METH-induced hyperactivity in mice[6]
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Dosage:7.5 μg
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Administration:i.c.v.
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Result:Reduced acute hyperactivity and attenuated expression of sensitization.
Chemical Information
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CAS No. 2610-05-1
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Appearance Solid
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Molecular Weight 992.80
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Formula C34H24N6Na4O16S4
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Color Light blue to blue
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SMILES
COC1=CC(C2=CC=C(/N=N/C3=C(O)C4=C(N)C(S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C4C=C3)C(OC)=C2)=CC=C1/N=N/C5=C(O)C6=C(N)C(S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C6C=C5
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Synonyms
Chicago Sky Blue 6B
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Cell Signal
TFAP2A-mediated downregulation of UBE2C is crucial for endometrial decidualization and embryo implantation. [Abstract]2025 Nov:135:112016. PMID: 40701315 -
Naunyn Schmiedebergs Arch Pharmacol
2025 Jun 12. PMID: 40500381 -
Solvent & Solubility
DMSO : 125 mg/mL (125.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 2.08 mg/mL (2.10 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (280 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
[1]. Lodha N, Poojary SA. A Novel Contrast Stain for the Rapid Diagnosis of Pityriasis Versicolor: A Comparison of Chicago Sky Blue 6B Stain, Potassium Hydroxide Mount and Culture. Indian J Dermatol. 2015;60(4):340-344. [Content Brief]
[2]. Yu G, et al. The antinociceptive effects of intracerebroventricular administration of Chicago sky blue 6B, a vesicular glutamate transporter inhibitor. Behav Pharmacol. 2013;24(8):653-658. [Content Brief]
[3]. Risse E, et al. Identification of a Compound That Disrupts Binding of Amyloid-β to the Prion Protein Using a Novel Fluorescence-based Assay. J Biol Chem. 2015;290(27):17020-17028. [Content Brief]
[4]. Min JO, et al. Chicago sky blue 6B inhibits α-synuclein aggregation and propagation. Mol Brain. 2022 Mar 28;15(1):27. [Content Brief]
[5]. Jin K, et al. Chicago sky blue 6B (CSB6B), an allosteric inhibitor of macrophage migration inhibitory factor (MIF), suppresses osteoclastogenesis and promotes osteogenesis through the inhibition of the NF-κB signaling pathway. Biochem Pharmacol. 2021 Oct;192:114734. [Content Brief]
[6]. He Z, et al. Chicago sky blue 6B, a vesicular glutamate transporters inhibitor, attenuates methamphetamine-induced hyperactivity and behavioral sensitization in mice. Behav Brain Res. 2013 Feb 15;239:172-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.0073 mL | 5.0363 mL | 10.0725 mL | 25.1813 mL |
| DMSO | 5 mM | 0.2015 mL | 1.0073 mL | 2.0145 mL | 5.0363 mL |
| 10 mM | 0.1007 mL | 0.5036 mL | 1.0073 mL | 2.5181 mL | |
| 15 mM | 0.0672 mL | 0.3358 mL | 0.6715 mL | 1.6788 mL | |
| 20 mM | 0.0504 mL | 0.2518 mL | 0.5036 mL | 1.2591 mL | |
| 25 mM | 0.0403 mL | 0.2015 mL | 0.4029 mL | 1.0073 mL | |
| 30 mM | 0.0336 mL | 0.1679 mL | 0.3358 mL | 0.8394 mL | |
| 40 mM | 0.0252 mL | 0.1259 mL | 0.2518 mL | 0.6295 mL | |
| 50 mM | 0.0201 mL | 0.1007 mL | 0.2015 mL | 0.5036 mL | |
| 60 mM | 0.0168 mL | 0.0839 mL | 0.1679 mL | 0.4197 mL | |
| 80 mM | 0.0126 mL | 0.0630 mL | 0.1259 mL | 0.3148 mL | |
| 100 mM | 0.0101 mL | 0.0504 mL | 0.1007 mL | 0.2518 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Direct Blue 1 Related Classifications
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