(9R,12aR)-AZD4747
Based on 1 Customer Validation
(9R,12aR)-AZD4747 is a diastereomer of AZD4747 (HY-154959). AZD4747 is a selective mutant GTPase KRASG12C inhibitor with blood-brain barrier permeability. AZD4747 has the potential to study cancer.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity : 98.90%
- 화학식: C24H22ClFN2O3
- 분자량:440.89
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
23.31 μM
Compound: 14
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Antiproliferative activity against human A549 cells harboring KRAS G12S mutant cultured as 3D spheroids assessed as reduction in cell proliferation incubated for 3 days by CellTiter-Glo 3D reagent based assay
Antiproliferative activity against human A549 cells harboring KRAS G12S mutant cultured as 3D spheroids assessed as reduction in cell proliferation incubated for 3 days by CellTiter-Glo 3D reagent based assay
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[PMID: 37395055] |
| NCI-H358 | IC50 |
0.012 μM
Compound: 14
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Antiproliferative activity against human NCI-H358 cells harboring KRAS G12C mutant cultured as 3D spheroids assessed as reduction in cell proliferation incubated for 3 days by CellTiter-Glo 3D reagent based assay
Antiproliferative activity against human NCI-H358 cells harboring KRAS G12C mutant cultured as 3D spheroids assessed as reduction in cell proliferation incubated for 3 days by CellTiter-Glo 3D reagent based assay
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[PMID: 37395055] |
| NCI-H358 | IC50 |
0.36 μM
Compound: 14
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Antiproliferative activity against human NCI-H358 cells harboring KRAS G12C mutant cultured as 2D spheroids assessed as reduction in cell proliferation incubated for 3 days by CellTiter-Glo reagent based assay
Antiproliferative activity against human NCI-H358 cells harboring KRAS G12C mutant cultured as 2D spheroids assessed as reduction in cell proliferation incubated for 3 days by CellTiter-Glo reagent based assay
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[PMID: 37395055] |
Chemical Information
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Appearance Solid
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분자량 440.89
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화학식 C24H22ClFN2O3
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Color White to off-white
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SMILES
C=CC(N(C[C@@H]1COC2=C3F)CCN1CC2=CC(C#CC)=[C@@]3[C@]4=C(C=CC=C4O)Cl)=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
In Vitro:
DMSO : 125 mg/mL (283.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
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Transepithelial/transendothelial electrical resistance assay
TEER measures electrical resistance across epithelial or endothelial monolayers cultured on permeable supports, and the readout reflects ionic conductance through the cell barrier, especially the paracellular pathway regulated by junctional integrity. TEER can be measured without destroying the monolayer and is commonly used before or during transport, permeability, barrier-disruption, and barrier-maturation experiments. TEER values are influenced by biological maturation and technical conditions; reported factors include temperature, medium formulation, passage number, electrode geometry, membrane properties, and junctional length during early monolayer maturation. Therefore, TEER should be interpreted with blank-insert subtraction, area normalization, repeated readings, and, when possible, orthogonal barrier readouts such as FITC-dextran flux or tight-junction staining.
순도&문서
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2681 mL | 11.3407 mL | 22.6814 mL | 56.7035 mL |
| 5 mM | 0.4536 mL | 2.2681 mL | 4.5363 mL | 11.3407 mL | |
| 10 mM | 0.2268 mL | 1.1341 mL | 2.2681 mL | 5.6703 mL | |
| 15 mM | 0.1512 mL | 0.7560 mL | 1.5121 mL | 3.7802 mL | |
| 20 mM | 0.1134 mL | 0.5670 mL | 1.1341 mL | 2.8352 mL | |
| 25 mM | 0.0907 mL | 0.4536 mL | 0.9073 mL | 2.2681 mL | |
| 30 mM | 0.0756 mL | 0.3780 mL | 0.7560 mL | 1.8901 mL | |
| 40 mM | 0.0567 mL | 0.2835 mL | 0.5670 mL | 1.4176 mL | |
| 50 mM | 0.0454 mL | 0.2268 mL | 0.4536 mL | 1.1341 mL | |
| 60 mM | 0.0378 mL | 0.1890 mL | 0.3780 mL | 0.9451 mL | |
| 80 mM | 0.0284 mL | 0.1418 mL | 0.2835 mL | 0.7088 mL | |
| 100 mM | 0.0227 mL | 0.1134 mL | 0.2268 mL | 0.5670 mL |
(9R,12aR)-AZD4747 Related Classifications
HY-154959A Related Classifications
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