SF3A3 - splicing factor 3a subunit 3 Gene

Also Known as PRP9; PRPF9; SAP61; SF3a60

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 10946

About SF3A3

Cytogenetic location: 1p34.3 Genomic coordinates (GRCh38): 1:37,956,975-37,990,022 (from NCBI)

This gene has 8 transcripts (splice variants), 219 orthologues and 1 paralogue. Ubiquitous expression in bone marrow (RPKM 22.5), lymph node (RPKM 20.6) and 25 other tissues.

Summary

This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

SF3A3 Products (2)

mRNA	Protein	Name
NM_001320830.2	NP_001307759.1	splicing factor 3A subunit 3 isoform 2
NM_006802.4	NP_006793.1	splicing factor 3A subunit 3 isoform 1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables protein binding	IPI IPI: Inferred from physical interaction	17098193	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in U2-type prespliceosome assembly	IDA IDA: Inferred from direct assay	11533230	GOA
involved in mRNA processing	IMP IMP: Inferred from mutant phenotype	15647371	GOA
involved in mRNA splicing, via spliceosome	IDA IDA: Inferred from direct assay	29360106	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of U2 snRNP	IDA IDA: Inferred from direct assay	11533230	GOA
part of U2-type precatalytic spliceosome	IDA IDA: Inferred from direct assay	8022796	GOA
part of U2-type spliceosomal complex	IDA IDA: Inferred from direct assay	32494006	GOA
part of U2-type spliceosomal complex	IPI IPI: Inferred from physical interaction	10882114	GOA
part of catalytic step 2 spliceosome	IDA IDA: Inferred from direct assay	11991638	GOA
located in nuclear speck	IDA IDA: Inferred from direct assay	8022796	GOA
located in nucleus	IDA IDA: Inferred from direct assay	21349847	GOA
part of spliceosomal complex	IDA IDA: Inferred from direct assay	9731529	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

SF3A3 Protein Structure

SF3a60_bindingd

Telomere_Sde2_2

DUF3449

0
100
200
300
400
501 a.a.

Protein Preferred Names	Protein Names
splicing factor 3A subunit 3	SAP 61

SF3A3 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	SF3A3	Q12874	SF3A1	Homo sapiens	Q15459	Crosslink	30021884
Intra	SF3A3	Q12874	SF3A1	Homo sapiens	Q15459	NMR	17098193
Intra	SF3A3	Q12874	SF3A1	Homo sapiens	Q15459	Pull Down	17098193
Intra	SF3A3	Q12874	SF3A1	Homo sapiens	Q15459	Anti Tag CoIP	35271311
Intra	SF3A3	Q12874	JPH3	Homo sapiens	Q8WXH2	Y2H Pooling	32814053
Intra	SF3A3	Q12874	JPH3	Homo sapiens	Q8WXH2	Validated Y2H	32814053
Intra	SF3A3	Q12874	JPH3	Homo sapiens	Q8WXH2	Y2H Array	32814053
Intra	SF3A3	Q12874	ZNF212	Homo sapiens	Q9UDV6	Y2H Array	31515488
Intra	SF3A3	Q12874	ZNF212	Homo sapiens	Q9UDV6	Validated Y2H	25416956
Intra	SF3A3	Q12874	SF3A2	Homo sapiens	Q15428	Affinity Chrom	17332742
Intra	SF3A3	Q12874	SF3B3	Homo sapiens	Q15393	Anti Tag CoIP	35271311
Intra	SF3A3	Q12874	TADA2A	Homo sapiens	O75478	Y2H Prey Pooling	25416956
Intra	SF3A3	Q12874	TADA2A	Homo sapiens	O75478	Y2H Array	25416956
Intra	SF3A3	Q12874	TRIM69	Homo sapiens	Q86WT6	Y2H Prey Pooling	25416956
Intra	SF3A3	Q12874	TRIM69	Homo sapiens	Q86WT6	Y2H Array	25416956

Cross: Cross-species interaction Intra: Intraspecies interaction

SF3A3 Antibodies

Cat. No.	상품명	신청	Reactivity
HY-P82733	SF3A3 Antibody (YA2478)	WB	Human, Mouse, Rat, Hamster
HY-P82733A	SF3A3 Antibody (YA2478)(PBS only)	WB	Human, Mouse, Rat, Hamster

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (5)

Cat. No.	상품명	효과	Purity	Rare Diseases
HY-114461B	(3S,17S)-FD-895		/	Unkown
HY-RS12759	SF3A3 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS12762	SF3B3 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS20489	Sf3b3 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS26997	Sf3b3 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Macaca mulatta	SF3A3	VGNC	VGNC:97824
Felis catus	SF3A3	VGNC	VGNC:65054
Mus musculus	SF3A3	MGD	MGI:1922312
Rattus norvegicus	SF3A3	RGD	RGD:1308726
Bos taurus	SF3A3	VGNC	VGNC:34508
Others	SF3A3	NCBI