Methylboronic acid
Based on 1 Customer Validation
Methylboronic acid is a synthetic boric acid. Methylboronic acid alleviates boron deficiency symptoms in Arabidopsis thaliana and promotes the growth of taproots, lateral roots, root hairs and leaf surface area. Methylboronic acid is less toxic than boric acid at high concentrations, only causing a slight shortening of the taproot length in Arabidopsis thaliana without reducing the total chlorophyll content. Methylboronic acid can be used in studies related to boron deficiency in plants.
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- Purity: 98.0%
- CAS No.: 13061-96-6
- 화학식: CH5BO2
- 분자량:59.86
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보관:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
>500 μM
Compound: Table 1, R38C1
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Inhibition of ORAl1/2/3 in HEK293 cells assessed as inhibition of Ca2+ influx after 10 mins by FLIPR assay in presence of thapsigargin
Inhibition of ORAl1/2/3 in HEK293 cells assessed as inhibition of Ca2+ influx after 10 mins by FLIPR assay in presence of thapsigargin
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[PMID: 23602525] |
| HEK293 | IC50 |
>500 μM
Compound: Table 1, R38C1
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Inhibition of ORAl1/2/3 in HEK293 cells assessed as inhibition of Cd2+ influx after 10 mins by FLIPR assay in presence of thapsigargin
Inhibition of ORAl1/2/3 in HEK293 cells assessed as inhibition of Cd2+ influx after 10 mins by FLIPR assay in presence of thapsigargin
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[PMID: 23602525] |
Methylboronic acid (MBA) (50 μM; 7-14 days) alleviates boron deficiency symptoms and promotes the growth of Arabidopsis thaliana Col-0, Ler, and C24 ecotypes; compared with plants treated with boric acid 14 days after transplantation, its primary roots are significantly longer, lateral root number is greater, root hairs are longer, and leaf area is larger[1].
Methylboronic acid exhibits lower high-concentration toxicity than boric acid in Arabidopsis thaliana[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 13061-96-6
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Appearance Solid
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분자량 59.86
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화학식 CH5BO2
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Color White to off-white
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SMILES
CB(O)O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
용액&용해도
DMSO : 100 mg/mL (1670.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (41.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (41.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (272 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 16.7056 mL | 83.5282 mL | 167.0565 mL | 417.6412 mL |
| 5 mM | 3.3411 mL | 16.7056 mL | 33.4113 mL | 83.5282 mL | |
| 10 mM | 1.6706 mL | 8.3528 mL | 16.7056 mL | 41.7641 mL | |
| 15 mM | 1.1137 mL | 5.5685 mL | 11.1371 mL | 27.8427 mL | |
| 20 mM | 0.8353 mL | 4.1764 mL | 8.3528 mL | 20.8821 mL | |
| 25 mM | 0.6682 mL | 3.3411 mL | 6.6823 mL | 16.7056 mL | |
| 30 mM | 0.5569 mL | 2.7843 mL | 5.5685 mL | 13.9214 mL | |
| 40 mM | 0.4176 mL | 2.0882 mL | 4.1764 mL | 10.4410 mL | |
| 50 mM | 0.3341 mL | 1.6706 mL | 3.3411 mL | 8.3528 mL | |
| 60 mM | 0.2784 mL | 1.3921 mL | 2.7843 mL | 6.9607 mL | |
| 80 mM | 0.2088 mL | 1.0441 mL | 2.0882 mL | 5.2205 mL | |
| 100 mM | 0.1671 mL | 0.8353 mL | 1.6706 mL | 4.1764 mL |
Methylboronic acid Related Classifications
HY-34449 Related Classifications
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