WH-15
Based on 1 Customer Validation
WH-15 is a fluorogenic PLC reporter with Km value of 49; 30, 86.1 µM for PLC-γ1, PLC-δ1, PLC-β2, respectively. WH-15 can be cleaved in a cascade reaction to generate fluorescent 6-aminoquinoline. WH-15 can be used to respond to imaging PLC activity in live cells (Ex/Em = ~344/530 nm).
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- Purity: 99.51%
- CAS No.: 1264748-47-1
- 화학식: C31H43N2O18P3
- 분자량:824.60
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보관:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
All Phospholipase Isoforms
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Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 25 mM (optimized according to the experiment).
1.2 Preparation of working solution
Dilute to 50 μM with assay buffer.
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
2.1 HEK-293T cells are transfected with 600 ng of the indicated DNA and 100 ng of empty vector.
2.2 Add 70 μL of 50 μM WH-15 (83 μg/mL fatty-acid free BSA, 50 mM HEPES (pH 7.2), 70 mM KCl, 3 mM CaCl2, 3 mM EGTA and 2 mM DTT) to 50 μL normalized cell lysate at 37°C.
2.3 Data is collected every 3 min using a Multilabel Counter (Ex/Em = 340/535 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1264748-47-1
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Appearance Solid
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분자량 824.60
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화학식 C31H43N2O18P3
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Color Light yellow to yellow
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SMILES
O[C@@H]1[C@H](OP(O)(OC2=CC=C(COC(NC3=CC=C4N=CC=CC4=C3)=O)C=C2OCCCCCCCC)=O)[C@@H](O)[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@@H]1O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
용액&용해도
DMSO : 25 mg/mL (30.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2127 mL | 6.0635 mL | 12.1271 mL | 30.3177 mL |
| 5 mM | 0.2425 mL | 1.2127 mL | 2.4254 mL | 6.0635 mL | |
| 10 mM | 0.1213 mL | 0.6064 mL | 1.2127 mL | 3.0318 mL | |
| 15 mM | 0.0808 mL | 0.4042 mL | 0.8085 mL | 2.0212 mL | |
| 20 mM | 0.0606 mL | 0.3032 mL | 0.6064 mL | 1.5159 mL | |
| 25 mM | 0.0485 mL | 0.2425 mL | 0.4851 mL | 1.2127 mL | |
| 30 mM | 0.0404 mL | 0.2021 mL | 0.4042 mL | 1.0106 mL |
WH-15 Related Classifications
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