Lignin Staining Solution (Pianeze Method)

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Staining Protocol
1.Sectioning & Staining: Prepare thin sections (10-25 µm) of plant material from 70% ethanol and stain in Pianese III-b solution for 18 to 24 hours at room temperature.
2.Differentiation & Clearing: Differentiate with acidified picric acid in 95% ethanol until the wash is pale yellow/light green, then dehydrate through ethanol, clear in xylene, and mount in a permanent medium.
3.Results Interpretation
(1) Lignified/Secondary Walls: Appear in distinct yellowish-green to deep green shades.
(2) Primary Cell Walls & Cytoplasm: Delineated sharply in red/pink hues.

The Lignin Staining Solution (Pianeze Method, specifically Pianeze III b) stains suberin and lignin identically in flax root cortex sections, with no differential staining observed between resistant, partially resistant, and susceptible flax varieties^[1].
Lignin Staining Solution (Pianeze Method) differentially stains lignified and unlignified cell wall components in Helminthostachys zeylanica rhizome and root, revealing spindle-shaped pit tori, and stains swollen pit membranes yellow in discolored Botrychium obliquum rhizome tracheids^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.