MART-1 (27-35) (human)
Based on 1 Customer Validation
MART-1 (27-35) (human) is the amino acid fragment spanning positions 27 to 35 of the MART-1 protein, and it represents an immunogenic epitope recognizable by HLA-A2-restricted melanoma-specific tumor-infiltrating lymphocytes (TILs). MART-1 (27-35) (human) can be used in studies related to melanoma.
For research use only. We do not sell to patients.
- Purity: 98.60%
- CAS No.: 156251-11-5
- Formula: C37H67N9O11
- Molecular Weight:813.98
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
MART-1 (27-35) (human) (0.1-10 μM; 2 h at room temperature then 24 h at 4 °C) binds to purified human HLA-A2 molecules, and this binding is detectable via fluorescent signals[1].
MART-1 (27-35) (human) (10 μM; overnight at 4 °C for initial complex formation) forms an HLA-A2 complex, which almost completely dissociates within 24 h at 37 °C[1].
MART-1 (27-35) (human) (1 μg/mL; 1 h incubation with T2 cells, 4 h cytolytic assay) is specifically recognized by human MART-1 (27-35)-specific, HLA-A2-restricted TIL clones LT8, LT11 and LT12, and induces T2 target cells to release high levels of 51Cr[1].
MART-1 (27-35) (human) (0.0001-10 μg/mL; 1 h) sensitizes HLA-A*0201+ T2 cells to lysis by CLW anti-MART-1 (27-35) CD8+ cytotoxic T lymphocytes (CTLs) in a dose-dependent manner[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 156251-11-5
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Appearance Solid
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Molecular Weight 813.98
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Formula C37H67N9O11
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Color White to off-white
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Sequence
Ala-Ala-Gly-Ile-Gly-Ile-Leu-Thr-Val
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Sequence Shortening
AAGIGILTV
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (122.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Guichard G, et al. Melanoma peptide MART-1(27-35) analogues with enhanced binding capacity to the human class I histocompatibility molecule HLA-A2 by introduction of a beta-amino acid residue: implications for recognition by tumor-infiltrating lymphocytes. J Med Chem. 2000;43(20):3803-3808. [Content Brief]
[2]. Loftus DJ, et al. Identification of epitope mimics recognized by CTL reactive to the melanoma/melanocyte-derived peptide MART-1(27-35). J Exp Med. 1996;184(2):647-657. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2285 mL | 6.1427 mL | 12.2853 mL | 30.7133 mL |
| 5 mM | 0.2457 mL | 1.2285 mL | 2.4571 mL | 6.1427 mL | |
| 10 mM | 0.1229 mL | 0.6143 mL | 1.2285 mL | 3.0713 mL | |
| 15 mM | 0.0819 mL | 0.4095 mL | 0.8190 mL | 2.0476 mL | |
| 20 mM | 0.0614 mL | 0.3071 mL | 0.6143 mL | 1.5357 mL | |
| 25 mM | 0.0491 mL | 0.2457 mL | 0.4914 mL | 1.2285 mL | |
| 30 mM | 0.0410 mL | 0.2048 mL | 0.4095 mL | 1.0238 mL | |
| 40 mM | 0.0307 mL | 0.1536 mL | 0.3071 mL | 0.7678 mL | |
| 50 mM | 0.0246 mL | 0.1229 mL | 0.2457 mL | 0.6143 mL | |
| 60 mM | 0.0205 mL | 0.1024 mL | 0.2048 mL | 0.5119 mL | |
| 80 mM | 0.0154 mL | 0.0768 mL | 0.1536 mL | 0.3839 mL | |
| 100 mM | 0.0123 mL | 0.0614 mL | 0.1229 mL | 0.3071 mL |
MART-1 (27-35) (human) Related Classifications
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