Methylene blue azide
Methylene blue azide is a derivative of a thiazine dye containing an azide group, used in click chemistry reactions (CuAAC) . Methylene blue azide is suitable for click chemistry-based bioconjugation (Ex/Em = 664/682 nm) .
For research use only. We do not sell to patients.
- Formula: C23H26F3N7O3S
- Molecular Weight:537.56
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
-
Help & FAQs
Biological Activity
Description
Chemical Information
-
Molecular Weight 537.56
-
Formula C23H26F3N7O3S
-
SMILES
CN(C)C1=CC2=[S+]C3=C(C=CC(N(C)CCC(NCCCN=[N+]=[N-])=O)=C3)N=C2C=C1.FC(F)(F)C([O-])=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Methylene blue azide Related Classifications
HY-D3478 Related Classifications
Powered by Bioz