Milliamine C
Milliamine C is a diterpenoid ester of the euphane type that inhibits the specific binding of phorbol-12,13-dipropionate to mouse epidermal particulate fractions and induces skin irritation, but exhibits very weak or undetectable tumor-promoting activity. Milliamine C can be used in studies related to skin tumor promotion.
For research use only. We do not sell to patients.
- CAS No.: 49620-09-9
- Formula: C43H47N3O9
- Molecular Weight:749.85
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Milliamine C (compound 4) inhibits the binding of [3H]-PDPr to cellular diterpene ester receptors[1].
Milliamine C (10 nM; 20 min) strongly inhibits the specific binding of [3H]-PDPr to epidermal particulate fractions from NMRI mice, with a Ki50 of 2.3 nM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
Milliamine C (topical; single administration) is a potent irritant on the mouse ear, with an ID5024 of 0.0066 nmol[1].
Milliamine C (topical; single administration; measured at 24 h) acts as a strong skin irritant in NMRI mice, with an ID5024 of 0.007 nmol/ear[2].
Milliamine C exhibits acute toxicity in mice, with an LD100 of 0.64 mg/kg[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NMRI mice[1]
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Dosage:20 nmol
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Administration:topical; twice per week
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Result:Exhibited no tumour promoting activity in the standard initiation/promotion experiment at a dose of 20 nmol applied twice per week.
Chemical Information
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CAS No. 49620-09-9
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Molecular Weight 749.85
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Formula C43H47N3O9
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SMILES
O=C(O[C@H]1C(C)=C[C@]23[C@]1(O)[C@H](O)C(CO)=C[C@](C3=O)([H])[C@@]4([H])[C@@](C4(C)C)([H])C[C@H]2C)C5=CC=CC=C5NC(C6=CC=CC(O)=C6NC(C7=CC=CC=C7N(C)C)=O)=O
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Dermal Irritation/Dermal Toxicity Study
This protocol assesses dermal irritation using reconstructed human epidermis (RhE) models such as EpiDerm, EPISKIN, and SkinEthic RHE, in which a test substance is applied topically and tissue viability is measured after exposure; reduced viability reflects cytotoxic injury associated with skin irritation potential. The primary readout is MTT reduction, where viable cells convert tetrazolium salt into colored formazan measured by spectrophotometry; this signal is used as a quantitative viability endpoint for classifying irritant versus non-irritant responses. The historical in vivo comparator is the Draize rabbit skin irritation method, which scores erythema and edema after topical exposure, but validated RhE assays were developed to replace or reduce reliance on this animal-based endpoint.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Milliamine C Related Classifications
HY-N21575 Related Classifications
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Keywords
- Milliamine C
- 49620-09-9
- Biochemical Assay Reagents
- NMRI mice
- ingenane diterpene ester
- Euphorbia milii
- 7,12-dimethylbenz[a]anthracene
- skin tumor promotion
- mouse ear tissue
- cellular diterpene ester receptors
- mouse epidermal particulate fractions
- phorbol-12,13-dipropionate binding site inhibitor
- mouse skin
- Inhibitor
- inhibitor
- inhibit