2. Cell Cycle/DNA Damage
  3. G-quadruplex
  4. N-Methylmesoporphyrin IX

N-Methylmesoporphyrin IX (NMM), a widely used G-quadruplex DNA specific fluorescent binder, is an efficient probe for monitoring Aβ fibrillation. N-Methylmesoporphyrin IX is an in situ inhibitor and an ex situ monitor for Aβ amyloidogenesis both in vitro and in cells. N-Methylmesoporphyrin IX is sensitive to G-quadruplexes DNA but has no response to duplexes, triplexes and single-stranded forms DNA. N-Methylmesoporphyrin IX is nonfluorescent alone or in monomeric Aβ environments, but emits strong fluorescence through stacking with the Aβ assemblies.

For research use only. We do not sell to patients.

CAS No. : 142234-85-3

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of N-Methylmesoporphyrin IX:

N-Methylmesoporphyrin IX Related Antibodies

Top Publications Citing Use of Products

    N-Methylmesoporphyrin IX purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Sep 11;32(9):1579-1593.e8.  [Abstract]

    Native TBE acrylamide gel analysis of the oligonucleotides with a G4 light-up probe N-Methylmesoporphyrin IX (NMM) (10 μg/mL; 15 min).

    N-Methylmesoporphyrin IX purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Sep 11;32(9):1579-1593.e8.  [Abstract]

    Native TBE gel analysis of oligonucleotides containing the WT or mutated PQS with a G4 light-up probe N-Methylmesoporphyrin IX (NMM) (10 μg/mL; 15 min).

    N-Methylmesoporphyrin IX purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Sep 11;32(9):1579-1593.e8.  [Abstract]

    N-Methylmesoporphyrin IX (NMM) (10 μg/mL; 15 min). Native gel analysis and corresponding G4s.

    N-Methylmesoporphyrin IX purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Sep 11;32(9):1579-1593.e8.  [Abstract]

    N-Methylmesoporphyrin IX (NMM) (10 μg/mL; 15 min). Native gel analysis of G4s in oligos containing the 5-B PQS sequences of the orthoflaviviruses.

    N-Methylmesoporphyrin IX purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Sep 11;32(9):1579-1593.e8.  [Abstract]

    N-Methylmesoporphyrin IX (NMM) (10 μg/mL; 15 min). Native gel analysis of tiRNAAla WT and mut stained for G4s.

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    • Purity & Documentation

    • References

    • Customer Review

    Description

    N-Methylmesoporphyrin IX (NMM), a widely used G-quadruplex DNA specific fluorescent binder, is an efficient probe for monitoring Aβ fibrillation. N-Methylmesoporphyrin IX is an in situ inhibitor and an ex situ monitor for Aβ amyloidogenesis both in vitro and in cells. N-Methylmesoporphyrin IX is sensitive to G-quadruplexes DNA but has no response to duplexes, triplexes and single-stranded forms DNA. N-Methylmesoporphyrin IX is nonfluorescent alone or in monomeric Aβ environments, but emits strong fluorescence through stacking with the Aβ assemblies[1].

    In Vitro

    Guide (The following is our recommended protocol; this is a guideline and should be modified according to your specific needs).
    1. Preparation of Aβ peptide fibrils.
    2. N-Methylmesoporphyrin IX (NMM) (10 μM) is co-incubated with Aβ peptide (50 μM) for 7 days at 37°C.
    3. Additional N-Methylmesoporphyrin IX is added before fluorescence measurements to maintain a constant N-Methylmesoporphyrin IX concentration (1 μM).
    4. Observed using a fluorescence microscope or flow cytometry (Ex/Em = 399/610 nm).

    N-Methylmesoporphyrin IX (NMM) is sensitive to G-quadruplexes DNA but has no response to duplexes, triplexes and single-stranded forms DNA. Upon binding to quadruplex DNA, for efficient π-π stacking, NMM can adjust its macrocycle geometry to match the terminal face of a G-quadruplex, leading to an enhancement in its fluorescence[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    N-Methylmesoporphyrin IX Related Antibodies
    Molecular Weight

    580.72

    Formula

    C35H40N4O4
    CAS No.
    Appearance

    Solid

    Color

    Brown to black

    SMILES

    O=C(O)CCC1=C2/C=C3C(CCC(O)=O)=C(C)C(/C=C(N/4C)/C(CC)=C(C)C4=C\C5=N/C(C(C)=C5CC)=C\C(N2)=C1C)=N/3.O=C(O)CCC6=C7/C=C8C(CCC(O)=O)=C(C)C(/C=C(N/9)/C(CC)=C(C)C9=C\C%10=N/C(C(C)=C%10CC)=C\C(N7C)=C6C)=N/8
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (43.05 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7220 mL 8.6100 mL 17.2200 mL
    5 mM 0.3444 mL 1.7220 mL 3.4440 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    In Vivo Dissolution Calculator
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    Purity & Documentation

    Purity: 99.65%

    SDS (393 KB)

    COA (252 KB) HNMR (365 KB) RP-HPLC (263 KB) MS (70 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.7220 mL 8.6100 mL 17.2200 mL 43.0500 mL
    5 mM 0.3444 mL 1.7220 mL 3.4440 mL 8.6100 mL
    10 mM 0.1722 mL 0.8610 mL 1.7220 mL 4.3050 mL
    15 mM 0.1148 mL 0.5740 mL 1.1480 mL 2.8700 mL
    20 mM 0.0861 mL 0.4305 mL 0.8610 mL 2.1525 mL
    25 mM 0.0689 mL 0.3444 mL 0.6888 mL 1.7220 mL
    30 mM 0.0574 mL 0.2870 mL 0.5740 mL 1.4350 mL
    40 mM 0.0431 mL 0.2153 mL 0.4305 mL 1.0763 mL
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    N-Methylmesoporphyrin IX Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    N-Methylmesoporphyrin IX142234-85-3G-quadruplexG-quadruplex DNAfluorescent binderAβ fibrillationsituAβ amyloidogenesisduplexestriplexessingle-strandedassembliesInhibitorinhibitorinhibit

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