N-Methylmesoporphyrin IX
Based on 5 publication(s) in Google Scholar
N-Methylmesoporphyrin IX (NMM), a widely used G-quadruplex DNA specific fluorescent binder, is an efficient probe for monitoring Aβ fibrillation. N-Methylmesoporphyrin IX is an in situ inhibitor and an ex situ monitor for Aβ amyloidogenesis both in vitro and in cells. N-Methylmesoporphyrin IX is sensitive to G-quadruplexes DNA but has no response to duplexes, triplexes and single-stranded forms DNA. N-Methylmesoporphyrin IX is nonfluorescent alone or in monomeric Aβ environments, but emits strong fluorescence through stacking with the Aβ assemblies.
For research use only. We do not sell to patients.
- Purity: 99.65%
- CAS No.: 142234-85-3
- Formula: C35H40N4O4
- Molecular Weight:580.72
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) N-Methylmesoporphyrin IX
More
Customer Validation & Images
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Gel Electrophoresis
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Gel Electrophoresis
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Gel Electrophoresis
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Gel Electrophoresis
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WB
Biological Activity
Guide (The following is our recommended protocol; this is a guideline and should be modified according to your specific needs).
1. Preparation of Aβ peptide fibrils.
2. N-Methylmesoporphyrin IX (NMM) (10 μM) is co-incubated with Aβ peptide (50 μM) for 7 days at 37°C.
3. Additional N-Methylmesoporphyrin IX is added before fluorescence measurements to maintain a constant N-Methylmesoporphyrin IX concentration (1 μM).
4. Observed using a fluorescence microscope or flow cytometry (Ex/Em = 399/610 nm).
N-Methylmesoporphyrin IX (NMM) is sensitive to G-quadruplexes DNA but has no response to duplexes, triplexes and single-stranded forms DNA. Upon binding to quadruplex DNA, for efficient π-π stacking, NMM can adjust its macrocycle geometry to match the terminal face of a G-quadruplex, leading to an enhancement in its fluorescence[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 142234-85-3
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Appearance Solid
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Molecular Weight 580.72
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Formula C35H40N4O4
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Color Brown to black
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SMILES
O=C(O)CCC1=C2/C=C3C(CCC(O)=O)=C(C)C(/C=C(N/4C)/C(CC)=C(C)C4=C\C5=N/C(C(C)=C5CC)=C\C(N2)=C1C)=N/3.O=C(O)CCC6=C7/C=C8C(CCC(O)=O)=C(C)C(/C=C(N/9)/C(CC)=C(C)C9=C\C%10=N/C(C(C)=C%10CC)=C\C(N7C)=C6C)=N/8
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (5)
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Journal Impact Factor
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Most Recent
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Cell Host Microbe
2024 Sep 11;32(9):1579-1593.e8. PMID: 39094585
N-Methylmesoporphyrin IX purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Sep 11;32(9):1579-1593.e8. [Abstract]
Native TBE acrylamide gel analysis of the oligonucleotides with a G4 light-up probe N-Methylmesoporphyrin IX (NMM) (10 μg/mL; 15 min).
N-Methylmesoporphyrin IX purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Sep 11;32(9):1579-1593.e8. [Abstract]
Native TBE gel analysis of oligonucleotides containing the WT or mutated PQS with a G4 light-up probe N-Methylmesoporphyrin IX (NMM) (10 μg/mL; 15 min).
N-Methylmesoporphyrin IX purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Sep 11;32(9):1579-1593.e8. [Abstract]
N-Methylmesoporphyrin IX (NMM) (10 μg/mL; 15 min). Native gel analysis and corresponding G4s.
N-Methylmesoporphyrin IX purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Sep 11;32(9):1579-1593.e8. [Abstract]
N-Methylmesoporphyrin IX (NMM) (10 μg/mL; 15 min). Native gel analysis of G4s in oligos containing the 5-B PQS sequences of the orthoflaviviruses.
N-Methylmesoporphyrin IX purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Sep 11;32(9):1579-1593.e8. [Abstract]
N-Methylmesoporphyrin IX (NMM) (10 μg/mL; 15 min). Native gel analysis of tiRNAAla WT and mut stained for G4s.
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Redox Biol
Oxidative modification of G-quadruplex triggers CLIC4-associated mitochondrial dysfunction to promote glioblastoma progression. [Abstract]2025 Dec:88:103917. PMID: 41265251 -
Biochem Biophys Res Commun
2025 Dec 9:795:153117. PMID: 41380445 -
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Solvent & Solubility
DMSO : 25 mg/mL (43.05 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7220 mL | 8.6100 mL | 17.2200 mL | 43.0500 mL |
| 5 mM | 0.3444 mL | 1.7220 mL | 3.4440 mL | 8.6100 mL | |
| 10 mM | 0.1722 mL | 0.8610 mL | 1.7220 mL | 4.3050 mL | |
| 15 mM | 0.1148 mL | 0.5740 mL | 1.1480 mL | 2.8700 mL | |
| 20 mM | 0.0861 mL | 0.4305 mL | 0.8610 mL | 2.1525 mL | |
| 25 mM | 0.0689 mL | 0.3444 mL | 0.6888 mL | 1.7220 mL | |
| 30 mM | 0.0574 mL | 0.2870 mL | 0.5740 mL | 1.4350 mL | |
| 40 mM | 0.0431 mL | 0.2153 mL | 0.4305 mL | 1.0763 mL |
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