N-Methylmesoporphyrin IX (solution)
Based on 1 Customer Validation
N-Methylmesoporphyrin IX (NMM) (solution) , a widely used G-quadruplex DNA specific fluorescent binder, is an efficient probe for monitoring Aβ fibrillation. N-Methylmesoporphyrin IX is an in situ inhibitor and an ex situ monitor for Aβ amyloidogenesis both in vitro and in cells. N-Methylmesoporphyrin IX is sensitive to G-quadruplexes DNA but has no response to duplexes, triplexes and single-stranded forms DNA. N-Methylmesoporphyrin IX is nonfluorescent alone or in monomeric Aβ environments, but emits strong fluorescence through stacking with the Aβ assemblies.
Solvent and concentration: DMSO: 2 mM
For research use only. We do not sell to patients.
- CAS No.: 142234-85-3
- Formula: C70H80N8O8
- Molecular Weight:1161.46
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Solvent and concentration: DMSO: 2 mM
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of solution preparation
Dissolve N-Methylmesoporphyrin IX in DMSO (5-10 mg/mL) or 0.2 M HCl.
2. G-Quadruplex Staining Protocol (Gel):
2.1 Fold DNA in a buffer containing KCl (e.g., 50 mM KCl).
2.2 Run electrophoresis on a 12-15% polyacrylamide gel.
2.3 Stain the gel with 10 μg/mL N-Methylmesoporphyrin IX in 1x G4 folding buffer for 15 min under agitation.
2.4 Keep the gel in the dark to avoid losing signal.
3. Solution Titration/Fluorescence Study:
3.1 Prepare N-Methylmesoporphyrin IX in working buffer (e.g., 10 mM lithium cacodylate, pH 7.2, 5 mM KCl, 95 mM LiCl).
3.2 Co-incubate N-Methylmesoporphyrin IX (e.g., 1 μM) with DNA (e.g., 50 μM).
3.3 Monitor fluorescence, ensuring proper KCl concentration for G4 folding.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 142234-85-3
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Appearance Liquid (Density: 1.261±0.14 g/cm3)
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Molecular Weight 1161.46
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Formula C70H80N8O8
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Color Dark purple to black
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SMILES
O=C(O)CCC1=C2/C=C3C(CCC(O)=O)=C(C)C(/C=C(N/4C)/C(CC)=C(C)C4=C\C5=N/C(C(C)=C5CC)=C\C(N2)=C1C)=N/3.O=C(O)CCC6=C7/C=C8C(CCC(O)=O)=C(C)C(/C=C(N/9)/C(CC)=C(C)C9=C\C%10=N/C(C(C)=C%10CC)=C\C(N7C)=C6C)=N/8
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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