2. Metabolic Enzyme/Protease
  3. Ferrochelatase
  4. N-Methylprotoporphyrin IX

N-Methylprotoporphyrin IX is a Ferrochelatase inhibitor with a Kiapp value of 3 nM. N-methylprotoporphyrin IX dimethyl ester potently inhibits the synthesis of phycocyanin and chlorophyll a. N-Methylprotoporphyrin IX can be used for the research of haem deficiency-associated disorders.

For research use only. We do not sell to patients.

N-Methylprotoporphyrin IX

N-Methylprotoporphyrin IX Chemical Structure

CAS No. : 79236-56-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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N-Methylprotoporphyrin IX Related Antibodies

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1 Publications Citing Use of MCE N-Methylprotoporphyrin IX

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Description

N-Methylprotoporphyrin IX is a Ferrochelatase inhibitor with a Kiapp value of 3 nM. N-methylprotoporphyrin IX dimethyl ester potently inhibits the synthesis of phycocyanin and chlorophyll a. N-Methylprotoporphyrin IX can be used for the research of haem deficiency-associated disorders[1][2].

IC50 & Target

ferrochelatase enzyme[1]
In Vitro

N-Methylprotoporphyrin IX (1 h) exhibits 17.8-fold and 33.3-fold lower binding affinities for the purified ferrochelatase P255R and P255G mutants, respectively, compared with wild-type ferrochelatase (the Kd values of the three are 0.16 ± 0.07 μM, 0.30 ± 0.10 μM, and 9 ± 5 nM, respectively)[1].
N-Methylprotoporphyrin IX inhibits ferrochelatase with an apparent inhibition constant (Kiapp) of 3 nM; in contrast, the P255R and P255G mutants show significantly reduced sensitivity to NMPP, with corresponding Kiapp values of 1 μM and 2.3 μM, respectively[1].
N-methylprotoporphyrin IX dimethyl ester (0.5-20 μM; up to 90 h) potently inhibits the synthesis of phycocyanin and chlorophyll a in C. caldarium cells cultured under light conditions[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

N-Methylprotoporphyrin IX Related Antibodies
Molecular Weight

576.68

Formula

C35H36N4O4
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

O=C(O)CCC1=C2/C=C3C(CCC(O)=O)=C(C)C(/C=C(N/4C)/C(C)=C(C=C)C4=C\C5=N/C(C(C=C)=C5C)=C\C(N2)=C1C)=N/3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 15 mg/mL (26.01 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7341 mL 8.6703 mL 17.3406 mL
5 mM 0.3468 mL 1.7341 mL 3.4681 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.0%

SDS (393 KB)

COA (261 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7341 mL 8.6703 mL 17.3406 mL 43.3516 mL
5 mM 0.3468 mL 1.7341 mL 3.4681 mL 8.6703 mL
10 mM 0.1734 mL 0.8670 mL 1.7341 mL 4.3352 mL
15 mM 0.1156 mL 0.5780 mL 1.1560 mL 2.8901 mL
20 mM 0.0867 mL 0.4335 mL 0.8670 mL 2.1676 mL
25 mM 0.0694 mL 0.3468 mL 0.6936 mL 1.7341 mL
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N-Methylprotoporphyrin IX Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Methylprotoporphyrin IX79236-56-9Ferrochelatasemammalian cellsCyanidium caldarium cellsmouseferrochelatasemitochondrial electron transport complex IVprotoporphyrin IXchlorophyll aphycocyaninEscherichia coli Δvis cellsphycocyanobilinInhibitorinhibitorinhibit

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