N-Methylprotoporphyrin IX
Based on 1 publication(s) in Google Scholar
N-Methylprotoporphyrin IX is a Ferrochelatase inhibitor with a Kiapp value of 3 nM. N-methylprotoporphyrin IX dimethyl ester potently inhibits the synthesis of phycocyanin and chlorophyll a. N-Methylprotoporphyrin IX can be used for the research of haem deficiency-associated disorders.
For research use only. We do not sell to patients.
- Purity: 99.0%
- CAS No.: 79236-56-9
- Formula: C35H36N4O4
- Molecular Weight:576.68
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) N-Methylprotoporphyrin IX
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Biological Activity
ferrochelatase enzyme[1]
N-Methylprotoporphyrin IX (1 h) exhibits 17.8-fold and 33.3-fold lower binding affinities for the purified ferrochelatase P255R and P255G mutants, respectively, compared with wild-type ferrochelatase (the Kd values of the three are 0.16 ± 0.07 μM, 0.30 ± 0.10 μM, and 9 ± 5 nM, respectively)[1].
N-Methylprotoporphyrin IX inhibits ferrochelatase with an apparent inhibition constant (Kiapp) of 3 nM; in contrast, the P255R and P255G mutants show significantly reduced sensitivity to NMPP, with corresponding Kiapp values of 1 μM and 2.3 μM, respectively[1].
N-methylprotoporphyrin IX dimethyl ester (0.5-20 μM; up to 90 h) potently inhibits the synthesis of phycocyanin and chlorophyll a in C. caldarium cells cultured under light conditions[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 79236-56-9
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Appearance Solid
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Molecular Weight 576.68
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Formula C35H36N4O4
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Color Brown to black
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SMILES
O=C(O)CCC1=C2/C=C3C(CCC(O)=O)=C(C)C(/C=C(N/4C)/C(C)=C(C=C)C4=C\C5=N/C(C(C=C)=C5C)=C\C(N2)=C1C)=N/3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Cell Host Microbe
Rothia mucilaginosa membrane vesicles stabilize labile iron to alleviate UVB-induced ferroptosis. [Abstract]2026 Jan 14;34(1):35-51.e9. PMID: 41500222
Solvent & Solubility
DMSO : ≥ 15 mg/mL (26.01 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Shi Z, et al. Modulation of inhibition of ferrochelatase by N-methylprotoporphyrin. Biochem J. 2006;399(1):21-28. [Content Brief]
[2]. Brown SB, et al. The effect of N-methylprotoporphyrin IX on the synthesis of photosynthetic pigments in Cyanidium caldarium. Further evidence for the role of haem in the biosynthesis of plant billins. Biochem J. 1982 Nov 15;208(2):487-91. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7341 mL | 8.6703 mL | 17.3406 mL | 43.3516 mL |
| 5 mM | 0.3468 mL | 1.7341 mL | 3.4681 mL | 8.6703 mL | |
| 10 mM | 0.1734 mL | 0.8670 mL | 1.7341 mL | 4.3352 mL | |
| 15 mM | 0.1156 mL | 0.5780 mL | 1.1560 mL | 2.8901 mL | |
| 20 mM | 0.0867 mL | 0.4335 mL | 0.8670 mL | 2.1676 mL | |
| 25 mM | 0.0694 mL | 0.3468 mL | 0.6936 mL | 1.7341 mL |
N-Methylprotoporphyrin IX Related Classifications
HY-129430 Related Classifications
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