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N,N'-Dinitrosopiperazine (Synonyms: 1,4-Dinitrosopiperazine; DNP)

Cat. No.: HY-136433
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N,N'-Dinitrosopiperazine (1,4-Dinitrosopiperazine; DNP) is a carcinogen with specificity for nasopharyngeal epithelium and facilitates NPC metastasis. N,N'-Dinitrosopiperazine regulates multiple signaling pathways through protein phosphorylation, including LYRIC at serine 568.

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N,N'-Dinitrosopiperazine Chemical Structure

N,N'-Dinitrosopiperazine Chemical Structure

CAS No. : 140-79-4

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Description

N,N'-Dinitrosopiperazine (1,4-Dinitrosopiperazine; DNP) is a carcinogen with specificity for nasopharyngeal epithelium and facilitates NPC metastasis. N,N'-Dinitrosopiperazine regulates multiple signaling pathways through protein phosphorylation, including LYRIC at serine 568[1].

In Vitro

N,N'-Dinitrosopiperazine (0.5-100 μM; 48 hours) has no inhibitory effects on the labeled 6-10B cells, and LDH activity is not significantly altered by DNP treatment in the 0.5-8 μM concentration range. However, it is cytotoxic from the concentration 10 μM[1].
N,N'-Dinitrosopiperazine (2-8 μM; 24 hours) induces 6-10B cell invasion and motility in a dose-dependent manner. At 6 μM, when compares to the control group, DNP increases cell invasion at 421.7% and cell motility is increased by 328.2%[1].
N,N'-Dinitrosopiperazine (6 μM; 24 hours) increases the expression of phospho-LYRIC s568 and LYRIC expression in CNE1 cells[1].

Cell Viability Assay[1]

Cell Line: The labeled 6-10B cells
Concentration: 0.5, 1, 2, 4, 6, 8, 10, 20, 40, 80, or 100 μM
Incubation Time: 48 hours
Result: Had no inhibitory effects at the concentration 0-8 μM.

Western Blot Analysis[1]

Cell Line: The NPC cell line CNE1
Concentration: 6 μM
Incubation Time: 24 hours
Result: Increased phospho-LYRIC s568 and LYRIC expression.
In Vivo

N,N'-Dinitrosopiperazine (injected into the tail veins; 40 mg/kg; 30 days) inhibits cell motility and invasion, and facilitates NPC metastasis in vivo. From a IHC result, Phospho-LYRIC expression is higher in the metastatic tumors of DNP-treated mice than in those of the untreated control mice[1].

Animal Model: BABL/c nude mice injected with labeled 6-10B cell suspensions (1 × 104 cells) with or without DNP(40 mg/kg)[1]
Dosage: 40 mg/kg
Administration: Injected into the tail veins; 30 days
Result: Induced LYRIC phosphorylation at serine 568 associated with NPC metastasis in vivo.
Molecular Weight

144.13

Formula

C₄H₈N₄O₂

CAS No.

140-79-4

SMILES

O=NN1CCN(N=O)CC1

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Keywords:

N,N'-Dinitrosopiperazine1,4-DinitrosopiperazineDNPOthersNPCcell6-10BcarcinogennasopharyngealepitheliummetastasisInhibitorinhibitorinhibit

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