2. Metabolic Enzyme/Protease Vitamin D Related/Nuclear Receptor
  3. ROR
  4. Neoruscogenin

Neoruscogenin, a member of the steroidal sapogenin family, is a high-affinity agonist of the nuclear receptor RORα (NR1F1) (EC50 = 0.11 µM).

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Neoruscogenin

Neoruscogenin 화학구조

CAS No. : 17676-33-4

사이즈 가격 재고 수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
1 mg 해외재고보유
5 mg 해외재고보유
10 mg 해외재고보유
25 mg 해외재고보유
50 mg 해외재고보유
100 mg 해외재고보유
200 mg   견적 받기  
500 mg   견적 받기  

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고객리뷰

Based on 1 publication(s) in Google Scholar

Other Forms of Neoruscogenin:

Neoruscogenin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Neoruscogenin

View All ROR Isoform Specific Products:

View All Isoforms
RORγ RORα RORβ

  • Biological Activity

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

Neoruscogenin, a member of the steroidal sapogenin family, is a high-affinity agonist of the nuclear receptor RORα (NR1F1) (EC50 = 0.11 µM)[1].

In Vitro

Neoruscogenin (0.1-10 μM) increases the cell viability, but has no effect on cell viability at 50 μM, and decreases the cell viability at 100 μM[2].
Neoruscogenin (0.1 μM, 5 days) promotes C2C12 myoblast differentiation[2].
Neoruscogenin (0.1 μM, 5 days) activates Akt/mTOR/rpS6 signaling pathway in C2C12 myoblasts, and inhibits the Atrogin-1 and MuRF-1 mRNA levels[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Neoruscogenin Related Antibodies

Western Blot Analysis[2]

Cell Line: C2C12 myoblasts
Concentration: 0.1 μM
Incubation Time: 5 days
Result: Increased protein expression of MyHC and MyOG at 0.1 μM, but did not work as well as 0.1 μM at 5 and 10 μM
In Vivo

Neoruscogenin (3 mg/kg,p.o. for seven days) activates hepatic RORα target gene (such as Bmal1, Cyp7b1, and G6Pase) expression in mice[1].
Neoruscogenin (1 mg/kg,i.p. every 2 days for 8 times) induces skeletal muscle hypertrophy in mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: male ICR mice[2]
Dosage: 1 mg/kg
Administration: i.p.
Result: Showd the larger size of TA muscles, and increasaed the weight of Gas muscles.
Showed visible myofibers hypertrophy (H&E staining).
분자량

428.60

화학식

C27H40O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]3(OC4)CCC4=C)([H])C[C@@]1([H])[C@@](CC=C(C[C@@H](O)C5)[C@@]6([C@@H]5O)C)([H])[C@]6([H])CC2
Structure Classification
Initial Source
선적

Room temperature in continental US; may vary elsewhere.
보관

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도
In Vitro: 

DMSO : 50 mg/mL (116.66 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3332 mL 11.6659 mL 23.3318 mL
5 mM 0.4666 mL 2.3332 mL 4.6664 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 0.56 mg/mL (1.31 mM); Clear solution

    This protocol yields a clear solution of ≥ 0.56 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.6 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 0.56 mg/mL (1.31 mM); Clear solution

    This protocol yields a clear solution of ≥ 0.56 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.6 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3332 mL 11.6659 mL 23.3318 mL 58.3294 mL
5 mM 0.4666 mL 2.3332 mL 4.6664 mL 11.6659 mL
10 mM 0.2333 mL 1.1666 mL 2.3332 mL 5.8329 mL
15 mM 0.1555 mL 0.7777 mL 1.5555 mL 3.8886 mL
20 mM 0.1167 mL 0.5833 mL 1.1666 mL 2.9165 mL
25 mM 0.0933 mL 0.4666 mL 0.9333 mL 2.3332 mL
30 mM 0.0778 mL 0.3889 mL 0.7777 mL 1.9443 mL
40 mM 0.0583 mL 0.2916 mL 0.5833 mL 1.4582 mL
50 mM 0.0467 mL 0.2333 mL 0.4666 mL 1.1666 mL
60 mM 0.0389 mL 0.1944 mL 0.3889 mL 0.9722 mL
80 mM 0.0292 mL 0.1458 mL 0.2916 mL 0.7291 mL
100 mM 0.0233 mL 0.1167 mL 0.2333 mL 0.5833 mL
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Neoruscogenin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Neoruscogenin17676-33-4RORRAR-related orphan receptorHepG2cellssteroidalsapogeninInhibitorinhibitorinhibit

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상품명:
Neoruscogenin
Cat. No.:
HY-N2253
수량:
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