Nicotinamide 1,N6-ethenoadenine dinucleotide
Based on 1 Customer Validation
Nicotinamide 1,N6-ethenoadenine dinucleotide (ε-NAD) is a fluorescent analog of NAD. Nicotinamide 1,N6-ethenoadenine dinucleotide can be cleaved by phosphodiesterase I (from C. adamanteus venom) and binds to bovine liver glutamate dehydrogenase. Nicotinamide 1,N6-ethenoadenine dinucleotide can serve as a substrate for G-ADP ribosylation of G proteins catalyzed by bacterial toxins. Nicotinamide 1,N6-ethenoadenine dinucleotide can be used as a fluorescent substrate for the study of ADP ribosylation reactions.
For research use only. We do not sell to patients.
- Purity: 99.05%
- CAS No.: 38806-38-1
- Formula: C23H27N7O14P2
- Molecular Weight:687.45
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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PDEI |
Nicotinamide 1,N6-ethenoadenine dinucleotide serves as a substrate for the bacterial toxin-catalyzed epsilon-ADP ribosylation of signal-transducing G-proteins[1].
Nicotinamide 1,N6-ethenoadenine dinucleotide can be cleaved into 5'-ε-AMP and nicotinamide mononucleotide by phosphodiesterase I (from C. adamanteus venom)[1].
Nicotinamide 1,N6-ethenoadenine dinucleotide binds to bovine liver glutamate dehydrogenase[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 38806-38-1
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Appearance Solid
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Molecular Weight 687.45
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Formula C23H27N7O14P2
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Color White to off-white
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SMILES
O[C@H]1[C@@H](O[C@@H]([C@H]1O)COP(OP(OC[C@H]2O[C@@H]([N+]3=CC=CC(C(N)=O)=C3)[C@@H]([C@@H]2O)O)([O-])=O)(O)=O)N4C5=C(N=C4)C6=NC=CN6C=N5
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Synonyms
ε-NAD
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 100 mg/mL (145.47 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Hingorani VN, et al. Fluorescent labeling of signal-transducing G-proteins. Pertussis toxin-catalyzed etheno-ADP ribosylation of transducin. J Biol Chem. 1988 Dec 25;263(36):19804-8. [Content Brief]
[2]. Favilla R, Mazzini A. The binding of 1,N6-etheno-NAD to bovine liver glutamate dehydrogenase. Biochim Biophys Acta. 1984 Jul 17;788(1):48-57. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.4547 mL | 7.2733 mL | 14.5465 mL | 36.3663 mL |
| 5 mM | 0.2909 mL | 1.4547 mL | 2.9093 mL | 7.2733 mL | |
| 10 mM | 0.1455 mL | 0.7273 mL | 1.4547 mL | 3.6366 mL | |
| 15 mM | 0.0970 mL | 0.4849 mL | 0.9698 mL | 2.4244 mL | |
| 20 mM | 0.0727 mL | 0.3637 mL | 0.7273 mL | 1.8183 mL | |
| 25 mM | 0.0582 mL | 0.2909 mL | 0.5819 mL | 1.4547 mL | |
| 30 mM | 0.0485 mL | 0.2424 mL | 0.4849 mL | 1.2122 mL | |
| 40 mM | 0.0364 mL | 0.1818 mL | 0.3637 mL | 0.9092 mL | |
| 50 mM | 0.0291 mL | 0.1455 mL | 0.2909 mL | 0.7273 mL | |
| 60 mM | 0.0242 mL | 0.1212 mL | 0.2424 mL | 0.6061 mL | |
| 80 mM | 0.0182 mL | 0.0909 mL | 0.1818 mL | 0.4546 mL | |
| 100 mM | 0.0145 mL | 0.0727 mL | 0.1455 mL | 0.3637 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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