Cat. No.: HY-Y0073

4-Hydroxyacetophenone

(Synonyms: P-hydroxyacetophenone)

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

4-Hydroxyacetophenone (P-hydroxyacetophenone) is a major hepatoprotective and choleretic compound found in Artemisia and Illicium plants, exhibiting antiviral and anti-inflammatory effects against hepatitis B virus. Additionally, 4-Hydroxyacetophenone inhibits cancer cell adhesion, invasion, and migration by remodeling actin. 4-Hydroxyacetophenone holds promise for research in the fields of inflammatory diseases and cancer.

For research use only. We do not sell to patients.

Purity: 99.88%
CAS No.: 99-93-4
Formula: C₈H₈O₂
Molecular Weight:136.15

Storage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

4-Hydroxyacetophenone Chemical Structure

CAS No.: 99-93-4

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Myosin Isoforms :

MLCK

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2 2.2.15	CC₅₀	2388.2 μM Compound: 1	Cytotoxicity against human HepG 2.2.15 cells by MTT assay Cytotoxicity against human HepG 2.2.15 cells by MTT assay	[PMID: 25737008]
HepG2 2.2.15	IC₅₀	1854.7 μM Compound: 1	Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as surface antigen HBeAg secretion after 72 hrs by ELISA Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as surface antigen HBeAg secretion after 72 hrs by ELISA	[PMID: 25737008]
HepG2 2.2.15	IC₅₀	306.4 μM Compound: 1	Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as decrease in viral DNA replication treated for 7 days by real time PCR analysis Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as decrease in viral DNA replication treated for 7 days by real time PCR analysis	[PMID: 25737008]
HepG2 2.2.15	IC₅₀	785.7 μM Compound: 1	Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as surface antigen HBsAg secretion after 72 hrs by ELISA Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as surface antigen HBsAg secretion after 72 hrs by ELISA	[PMID: 25737008]
N9	IC₅₀	>100 μM Compound: 29	Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 28073678]

In Vitro

4-Hydroxyacetophenone (P-hydroxyacetophenone) inhibits adhesion, invasion, and migration largely through NM2C activity in HCT116 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

HCT116 human colorectal cancer cell line
Concentration:

0-4 µM
Incubation Time:

72 h
Result:

Led to a significant dose-dependent decrease in adhesion, invasion, and migration of HCT116 cells at all concentrations, with migration being the most strongly inhibited among the three^[2].

In Vivo

4-Hydroxyacetophenone (P-hydroxyacetophenone) (1 mg/kg, s.i. daily redosing for 4 weeks) limits the development and spread of metastases in the colon cancer metastasis to the liver mice model^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Colon cancer metastasis to the liver mice model^[1]
Dosage:

1 mg/kg
Administration:

Splenic injection (s.i.), daily redosing for 4 weeks
Result:

Increased cortical tension in HCT116 cells and reduced the metastatic burden in vivo.

Chemical Information

CAS No. 99-93-4
Appearance Solid
Molecular Weight 136.15
Formula C₈H₈O₂
Color White to off-white
SMILES

CC(C1=CC=C(O)C=C1)=O
Synonyms

P-hydroxyacetophenone
Structure Classification
- Phenols
- Monophenols
- Ketones, Aldehydes, Acids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Publications (1)

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Journal Impact Factor

Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (734.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

7.3448 mL

36.7242 mL

73.4484 mL

5 mM

1.4690 mL

7.3448 mL

14.6897 mL

10 mM

0.7345 mL

3.6724 mL

7.3448 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (18.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (18.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (18.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ching-Wen C, et al. p-Hydroxyacetophenone suppresses nuclear factor-κB-related inflammation in nociceptive and inflammatory animal models. J Nat Med. 2017 Apr;71(2):422-432. [Content Brief]

[2]. Bryan DS, et al. 4-Hydroxyacetophenone modulates the actomyosin cytoskeleton to reduce metastasis. Proc Natl Acad Sci U S A. 2020 Sep 8;117(36):22423-22429. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	7.3448 mL	36.7242 mL	73.4484 mL	183.6210 mL
	5 mM	1.4690 mL	7.3448 mL	14.6897 mL	36.7242 mL
	10 mM	0.7345 mL	3.6724 mL	7.3448 mL	18.3621 mL
	15 mM	0.4897 mL	2.4483 mL	4.8966 mL	12.2414 mL
	20 mM	0.3672 mL	1.8362 mL	3.6724 mL	9.1811 mL
	25 mM	0.2938 mL	1.4690 mL	2.9379 mL	7.3448 mL
	30 mM	0.2448 mL	1.2241 mL	2.4483 mL	6.1207 mL
	40 mM	0.1836 mL	0.9181 mL	1.8362 mL	4.5905 mL
	50 mM	0.1469 mL	0.7345 mL	1.4690 mL	3.6724 mL
	60 mM	0.1224 mL	0.6121 mL	1.2241 mL	3.0604 mL
	80 mM	0.0918 mL	0.4591 mL	0.9181 mL	2.2953 mL
	100 mM	0.0734 mL	0.3672 mL	0.7345 mL	1.8362 mL

Keywords

4-Hydroxyacetophenone

1 Cited Publications

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4-Hydroxyacetophenone

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Publications Citing Use of MedChemExpress (MCE) 4-Hydroxyacetophenone

All Myosin Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

4-Hydroxyacetophenone Related Classifications

HY-Y0073 Related Classifications