Cat. No.: HY-B0275B

Oxytetracycline dihydrate

10 Cited Publications

Customer Review

Based on 10 publication(s) in Google Scholar

Oxytetracycline dihydrate is an antibiotic belonging to the tetracycline class. Oxytetracycline dihydrate potent inhibits Gram-negative and Gram-positive bacteria. Oxytetracycline dihydrate is a protein synthesis inhibitor and prevents the binding from aminoacil-tRNA to the complex m-ribosomal RNA. Oxytetracycline dihydrate also possesses anti-HSV-1 activity.

For research use only. We do not sell to patients.

Purity: 99.37%
CAS No.: 6153-64-6
Formula: C₂₂H₂₈N₂O₁₁
Molecular Weight:496.46

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Oxytetracycline dihydrate Chemical Structure

CAS No.: 6153-64-6

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (10)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]^[3]

Tetracycline

HSV-1

Bacterial

Microbial Metabolite

All HSV Isoforms :

All Antibiotic Isoforms :

All Endogenous Metabolite Isoforms :

In Vitro

Oxytetracycline is an important member of the bacterial aromatic polyketide family, which is a structurally diverse class of natural products. Oxytetracycline is synthesized by a type II polyketide synthase that generates the poly-beta-ketone backbone through successive decarboxylative condensation of malonyl-CoA extender units, followed by modifications by cyclases, oxygenases, transferases, and additional tailoring enzymes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The effects of administration a therapeutic dose of Oxytetracycline (82.8 mg/kg of bw to 1 % bw/day) for 10 days are species specific. Oxytetracycline increases the relative liver weight in Morone chrysops x M. saxatilis, the enzymatic activity of CYP3A4 in Ictalurus punctatus, protein expression of CYP3A4 in Oreochromis niloticus and depleted the hepatic CYP3A4 in the latter^[1].
For Oxytetracycline, the limits are 100 μg/kg in muscle and milk, 200 μg/kg in egg, 300 μg/kg in liver and 600 μg/kg in kidney. Oxytetracycline (OTC) is administered to fish as medicated feed at concentrations ranging from 35 to 75 mg a.i kg-1 biomass day-1 for 7-14 days^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 6153-64-6
Appearance Solid
Molecular Weight 496.46
Formula C₂₂H₂₈N₂O₁₁
Color Light yellow to yellow
SMILES

O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])[C@@H](O)[C@]3([H])[C@](C)(O)C4=C(C(C3=C(O)[C@@]21O)=O)C(O)=CC=C4)N.O.O
Structure Classification
- Phenols
- Monophenols
- Antibiotics
- Other Antibiotics
Initial Source
- Microorganisms
- Endogenous metabolite
Streptomyces rimosus
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (10)

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Journal Impact Factor

Theranostics

Antibiotic Azithromycin inhibits brown/beige fat functionality and promotes obesity in human and rodents. [Abstract]

2022 Jan 1;12(3):1187-1203. PMID: 35154482
Water Res

Quantifying community-wide antibiotic usage via urban water fingerprinting: Focus on contrasting resource settings in South Africa. [Abstract]

2023 Jul 15:240:120110. PMID: 37247434
Food Chem X

Machine learning prioritization of antibiotic residues in aquatic foods reveals exposure-driven genotoxic risk mediated by BCL2. [Abstract]

2026 Feb 8:34:103646. PMID: 41756597
J Hazard Mater Adv

2025 Nov 12;20:100939.
ACS Omega

Solvothermal Synthesis of Functionalized UiO-66/Fe3O4@CNCs for Selective Removal of Oxytetracycline. [Abstract]

2025 Sep 10;10(37):42791-42805. PMID: 41018641
Sci Rep

Qualitative immunoassay for the determination of tetracycline antibiotic residues in milk samples followed by a quantitative improved HPLC-DAD method. [Abstract]

2022 Aug 25;12(1):14502. PMID: 36008564
Saudi Pharm J

Determination of tetracycline, oxytetracycline and chlortetracycline residues in seafood products of Saudi Arabia using high performance liquid chromatography-Photo diode array detection. [Abstract]

2021 Jun;29(6):566-575. PMID: 34194263
Water

2025 Jun 6.
SSRN

2025 Dec 31.
Chemosphere

Mass-balance-model-based evaluation of sewage treatment plant contribution to residual pharmaceuticals in environmental waters. [Abstract]

2019 Jun:225:378-387. PMID: 30884299

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (100.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

1 M HCl : 10 mg/mL (20.14 mM; ultrasonic and adjust pH to 1 with 1 M HCl)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0143 mL

10.0713 mL

20.1426 mL

5 mM

0.4029 mL

2.0143 mL

4.0285 mL

10 mM

0.2014 mL

1.0071 mL

2.0143 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (563 KB)
- English - EN (563 KB)
- Français - FR (563 KB)
- Deutsch - DE (563 KB)
- Norwegian - NO (563 KB)
- Español - ES (563 KB)
- Swedish - SV (563 KB)
- Italian - IT (563 KB)
- Korean - KR (563 KB)
- Portuguese - PT (563 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Elia AC, et al. Transferability of oxytetracycline (OTC) from feed to carp muscle and evaluation of the antibiotic effects on antioxidant systems in liver and kidney. Fish Physiol Biochem. 2014 Aug;40(4):1055-68. [Content Brief]

[2]. Pickens LB, et al. Oxytetracycline biosynthesis. J Biol Chem. 2010 Sep 3;285(36):27509-15. [Content Brief]

[3]. Oguz Guvenmez, et al. A New Treatment Method for Herpes Simplex Virus Type 1-related Skin Lesions. Scientific & Academic. 2019; 8(1): 6-8.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
1 M HCl / DMSO	1 mM	2.0143 mL	10.0713 mL	20.1426 mL	50.3565 mL
	5 mM	0.4029 mL	2.0143 mL	4.0285 mL	10.0713 mL
	10 mM	0.2014 mL	1.0071 mL	2.0143 mL	5.0357 mL
	15 mM	0.1343 mL	0.6714 mL	1.3428 mL	3.3571 mL
	20 mM	0.1007 mL	0.5036 mL	1.0071 mL	2.5178 mL
DMSO	25 mM	0.0806 mL	0.4029 mL	0.8057 mL	2.0143 mL
	30 mM	0.0671 mL	0.3357 mL	0.6714 mL	1.6786 mL
	40 mM	0.0504 mL	0.2518 mL	0.5036 mL	1.2589 mL
	50 mM	0.0403 mL	0.2014 mL	0.4029 mL	1.0071 mL
	60 mM	0.0336 mL	0.1679 mL	0.3357 mL	0.8393 mL
	80 mM	0.0252 mL	0.1259 mL	0.2518 mL	0.6295 mL
	100 mM	0.0201 mL	0.1007 mL	0.2014 mL	0.5036 mL

Keywords

Oxytetracycline dihydrate

10 Cited Publications

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Oxytetracycline dihydrate

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Publications Citing Use of MedChemExpress (MCE) Oxytetracycline dihydrate

All HSV Isoforms

All Antibiotic Isoforms

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (10)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Oxytetracycline dihydrate Related Classifications

HY-B0275B Related Classifications