Oxytetracycline hydrochloride
Based on 10 publication(s) in Google Scholar
Oxytetracycline hydrochloride is an antibiotic belonging to the tetracycline class. Oxytetracycline hydrochloride potent inhibits Gram-negative and Gram-positive bacteria. Oxytetracycline hydrochloride is a protein synthesis inhibitor and prevents the binding from aminoacil-tRNA to the complex m-ribosomal RNA. Oxytetracycline hydrochloride also possesses anti-HSV-1 activity.
For research use only. We do not sell to patients.
- Purity: 98.92%
- CAS No.: 2058-46-0
- Formula: C22H25ClN2O9
- Molecular Weight:496.89
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Oxytetracycline hydrochloride
More- Theranostics. 2022 Jan 1;12(3):1187-1203. [Abstract]
- Water Res. 2023 Jul 15:240:120110. [Abstract]
- Food Chem X. 2026 Feb 8:34:103646. [Abstract]
- J Hazard Mater Adv. 2025 Nov 12;20:100939.
- ACS Omega. 2025 Sep 10;10(37):42791-42805. [Abstract]
- Sci Rep. 2022 Aug 25;12(1):14502. [Abstract]
- Saudi Pharm J. 2021 Jun;29(6):566-575. [Abstract]
- Water. 2025 Jun 6.
- SSRN. 2025 Dec 31.
- Chemosphere. 2019 Jun:225:378-387. [Abstract]
All Endogenous Metabolite Isoforms
MoreAll Antibiotic Isoforms
More
Biological Activity
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HSV-1 |
Microbial Metabolite |
Tetracycline |
Bacterial |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HepG2 | EC50 |
>64 μg/mL
Compound: OTC
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Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 4 hrs by CellTiter 96 Aqueous One Solution Reagent based assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 4 hrs by CellTiter 96 Aqueous One Solution Reagent based assay
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[PMID: 31536892] |
Oxytetracycline is an important member of the bacterial aromatic polyketide family, which is a structurally diverse class of natural products. Oxytetracycline is synthesized by a type II polyketide synthase that generates the poly-beta-ketone backbone through successive decarboxylative condensation of malonyl-CoA extender units, followed by modifications by cyclases, oxygenases, transferases, and additional tailoring enzymes[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
For Oxytetracycline, the limits are 100 μg/kg in muscle and milk, 200 μg/kg in egg, 300 μg/kg in liver and 600 μg/kg in kidney. Oxytetracycline (OTC) is administered to fish as medicated feed at concentrations ranging from 35 to 75 mg a.i kg-1 biomass day-1 for 7-14 days[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 2058-46-0
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Appearance Solid
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Molecular Weight 496.89
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Formula C22H25ClN2O9
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Color Light yellow to yellow
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SMILES
O[C@@](C(O)=C(C1=O)[C@@]2([H])[C@](O)(C3=CC=CC(O)=C13)C)(C4=O)[C@@]([C@@H](C(O)=C4C(N)=O)N(C)C)([H])[C@H]2O.Cl
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (10)
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Journal Impact Factor
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Most Recent
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Theranostics
Antibiotic Azithromycin inhibits brown/beige fat functionality and promotes obesity in human and rodents. [Abstract]2022 Jan 1;12(3):1187-1203. PMID: 35154482 -
Water Res
Quantifying community-wide antibiotic usage via urban water fingerprinting: Focus on contrasting resource settings in South Africa. [Abstract]2023 Jul 15:240:120110. PMID: 37247434 -
Food Chem X
Machine learning prioritization of antibiotic residues in aquatic foods reveals exposure-driven genotoxic risk mediated by BCL2. [Abstract]2026 Feb 8:34:103646. PMID: 41756597 -
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ACS Omega
Solvothermal Synthesis of Functionalized UiO-66/Fe3O4@CNCs for Selective Removal of Oxytetracycline. [Abstract]2025 Sep 10;10(37):42791-42805. PMID: 41018641 -
Sci Rep
Qualitative immunoassay for the determination of tetracycline antibiotic residues in milk samples followed by a quantitative improved HPLC-DAD method. [Abstract]2022 Aug 25;12(1):14502. PMID: 36008564 -
Saudi Pharm J
Determination of tetracycline, oxytetracycline and chlortetracycline residues in seafood products of Saudi Arabia using high performance liquid chromatography-Photo diode array detection. [Abstract]2021 Jun;29(6):566-575. PMID: 34194263 -
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Chemosphere
Mass-balance-model-based evaluation of sewage treatment plant contribution to residual pharmaceuticals in environmental waters. [Abstract]2019 Jun:225:378-387. PMID: 30884299
Solvent & Solubility
DMSO : 500 mg/mL (1006.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 4.17 mg/mL (8.39 mM); Clear solution
This protocol yields a clear solution of ≥ 4.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (41.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 4.17 mg/mL (8.39 mM); Clear solution
This protocol yields a clear solution of ≥ 4.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (41.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
[1]. Elia AC, et al. Transferability of oxytetracycline (OTC) from feed to carp muscle and evaluation of the antibiotic effects on antioxidant systems in liver and kidney. Fish Physiol Biochem. 2014 Aug;40(4):1055-68. [Content Brief]
[2]. Pickens LB, et al. Oxytetracycline biosynthesis. J Biol Chem. 2010 Sep 3;285(36):27509-15. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0125 mL | 10.0626 mL | 20.1252 mL | 50.3129 mL |
| 5 mM | 0.4025 mL | 2.0125 mL | 4.0250 mL | 10.0626 mL | |
| 10 mM | 0.2013 mL | 1.0063 mL | 2.0125 mL | 5.0313 mL | |
| 15 mM | 0.1342 mL | 0.6708 mL | 1.3417 mL | 3.3542 mL | |
| 20 mM | 0.1006 mL | 0.5031 mL | 1.0063 mL | 2.5156 mL | |
| 25 mM | 0.0805 mL | 0.4025 mL | 0.8050 mL | 2.0125 mL | |
| 30 mM | 0.0671 mL | 0.3354 mL | 0.6708 mL | 1.6771 mL | |
| 40 mM | 0.0503 mL | 0.2516 mL | 0.5031 mL | 1.2578 mL | |
| 50 mM | 0.0403 mL | 0.2013 mL | 0.4025 mL | 1.0063 mL | |
| 60 mM | 0.0335 mL | 0.1677 mL | 0.3354 mL | 0.8385 mL | |
| 80 mM | 0.0252 mL | 0.1258 mL | 0.2516 mL | 0.6289 mL | |
| 100 mM | 0.0201 mL | 0.1006 mL | 0.2013 mL | 0.5031 mL |
Oxytetracycline hydrochloride Related Classifications
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