Oxythiamine chloride hydrochloride
Based on 3 publication(s) in Google Scholar
Oxythiamine (Hydroxythiamine) chloride hydrochloride, an analogue of anti-metabolite, can suppress the non-oxidative synthesis of ribose and induce cell apoptosis. Oxythiamine chloride hydrochloride is a thiamine antagonist and inhibits transketolase (TK). Oxythiamine chloride hydrochloride inhibits cancer cell apoptosis and inhibits cell proliferation.
For research use only. We do not sell to patients.
- Purity: 96.11%
- CAS No.: 614-05-1
- Formula: C12H17Cl2N3O2S
- Molecular Weight:338.25
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Oxythiamine chloride hydrochloride
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
Vitamin B1, Thiamine, Transketolase[1].
Oxythiamine chloride hydrochloride (0-40 μM, 2 days) inhibits cell viability of MIA PaCa-2 cells (IC50: 14.95 μM)[1].
Oxythiamine chloride hydrochloride (0-500 μM, 48 h) suppresses expression of 14-3-3 protein beta/alpha in MIA PaCa-2 cells[1].
Oxythiamine chloride hydrochloride (0.1-100 μM, 6-48 h) inhibits A549 cell proliferation[3].
Oxythiamine chloride hydrochloride (0.1-100 μM, 24 h) induces A549 cell apoptosis[3].
Oxythiamine chloride hydrochloride (0–20 μM) inhibits the invasion and migration (IC50: 8.75 μM) of Lewis lung carcinoma (LLC) cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MIA PaCa-2 cells
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Concentration:0-40 μM
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Incubation Time:2 days
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Result:Inhibited cell viability with an IC50 of 14.95 μM.
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Cell Line:MIA PaCa-2 cells
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Concentration:0, 5, 50, 500 μM
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Incubation Time:48 h
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Result:Inhibited 14-3-3 protein beta/alpha expression, and increased alpha-enolase.
Oxythiamine chloride hydrochloride (250 or 500 mg/kg, daily for 5 week) inhibits tumor cell metastasis via inhibition of MMPs in mice implanted (s.c.) with LLC cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Ehrlich's ascites tumor hosting mice[2]
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Dosage:100-500 mg/kg
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Administration:i.p., 4 days
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Result:Inhibited tumor growth by 43% at 300 mg/kg and 84% at 500 mg/kg.
Chemical Information
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CAS No. 614-05-1
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Appearance Solid
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Molecular Weight 338.25
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Formula C12H17Cl2N3O2S
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Color White to off-white
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SMILES
CC1=C(CCO)SC=[N+]1CC2=CN=C(C)NC2=O.Cl.[Cl-]
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Synonyms
Hydroxythiamine chloride hydrochloride
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (3)
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Journal Impact Factor
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Most Recent
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Nat Commun
2022 Oct 17;13(1):6121. PMID: 36253417 -
Acta Physiol
Transketolase drives the development of aortic dissection by impairing mitochondrial bioenergetics. [Abstract]2024 Apr;240(4):e14113. PMID: 38380737 -
PLoS Pathog
White spot syndrome virus IE1 protein hijacks the host pentose phosphate pathway to fuel viral replication. [Abstract]2026 Jan 27;22(1):e1013913. PMID: 41592137
Solvent & Solubility
H2O : ≥ 100 mg/mL (295.64 mM)
DMSO : 50 mg/mL (147.82 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.15 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Wang J, et al. Inhibition of transketolase by oxythiamine altered dynamics of protein signals in pancreatic cancer cells. Exp Hematol Oncol. 2013 Jul 27;2:18. [Content Brief]
[2]. Raïs B, et al. Oxythiamine and dehydroepiandrosterone induce a G1 phase cycle arrest in Ehrlich's tumor cells through inhibition of the pentose cycle. FEBS Lett. 1999 Jul 30;456(1):113-8. [Content Brief]
[3]. Bai L, et al. A dose- and time-dependent effect of oxythiamine on cell growth inhibition in non-small cell lung cancer. Cogn Neurodyn. 2022 Jun;16(3):633-641. [Content Brief]
[4]. Yang CM, et al. The in vitro and in vivo anti-metastatic efficacy of oxythiamine and the possible mechanisms of action. Clin Exp Metastasis. 2010 May;27(5):341-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.9564 mL | 14.7820 mL | 29.5639 mL | 73.9098 mL |
| 5 mM | 0.5913 mL | 2.9564 mL | 5.9128 mL | 14.7820 mL | |
| 10 mM | 0.2956 mL | 1.4782 mL | 2.9564 mL | 7.3910 mL | |
| 15 mM | 0.1971 mL | 0.9855 mL | 1.9709 mL | 4.9273 mL | |
| 20 mM | 0.1478 mL | 0.7391 mL | 1.4782 mL | 3.6955 mL | |
| 25 mM | 0.1183 mL | 0.5913 mL | 1.1826 mL | 2.9564 mL | |
| 30 mM | 0.0985 mL | 0.4927 mL | 0.9855 mL | 2.4637 mL | |
| 40 mM | 0.0739 mL | 0.3695 mL | 0.7391 mL | 1.8477 mL | |
| 50 mM | 0.0591 mL | 0.2956 mL | 0.5913 mL | 1.4782 mL | |
| 60 mM | 0.0493 mL | 0.2464 mL | 0.4927 mL | 1.2318 mL | |
| 80 mM | 0.0370 mL | 0.1848 mL | 0.3695 mL | 0.9239 mL | |
| 100 mM | 0.0296 mL | 0.1478 mL | 0.2956 mL | 0.7391 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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