2. Apoptosis Metabolic Enzyme/Protease
  3. Apoptosis Endogenous Metabolite
  4. Oxythiamine chloride hydrochloride

Oxythiamine chloride hydrochloride  (Synonyms: Hydroxythiamine chloride hydrochloride)

Cat. No.: HY-107430A Purity: 96.36%
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Oxythiamine (Hydroxythiamine) chloride hydrochloride, an analogue of anti-metabolite, can suppress the non-oxidative synthesis of ribose and induce cell apoptosis. Oxythiamine chloride hydrochloride is a thiamine antagonist and inhibits transketolase (TK). Oxythiamine chloride hydrochloride inhibits cancer cell apoptosis and inhibits cell proliferation.

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Oxythiamine chloride hydrochloride Chemical Structure

Oxythiamine chloride hydrochloride Chemical Structure

CAS No. : 614-05-1

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Based on 2 publication(s) in Google Scholar

Other Forms of Oxythiamine chloride hydrochloride:

Oxythiamine chloride hydrochloride Related Antibodies

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2 Publications Citing Use of MCE Oxythiamine chloride hydrochloride

  • Biological Activity

  • Purity & Documentation

  • References

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Description

Oxythiamine (Hydroxythiamine) chloride hydrochloride, an analogue of anti-metabolite, can suppress the non-oxidative synthesis of ribose and induce cell apoptosis. Oxythiamine chloride hydrochloride is a thiamine antagonist and inhibits transketolase (TK). Oxythiamine chloride hydrochloride inhibits cancer cell apoptosis and inhibits cell proliferation[1][2][3].

IC50 & Target

Vitamin B1, Thiamine, Transketolase[1].
In Vitro

Oxythiamine chloride hydrochloride (0-40 μM, 2 days) inhibits cell viability of MIA PaCa-2 cells (IC50: 14.95 μM)[1].
Oxythiamine chloride hydrochloride (0-500 μM, 48 h) suppresses expression of 14-3-3 protein beta/alpha in MIA PaCa-2 cells[1].
Oxythiamine chloride hydrochloride (0.1-100 μM, 6-48 h) inhibits A549 cell proliferation[3].
Oxythiamine chloride hydrochloride (0.1-100 μM, 24 h) induces A549 cell apoptosis[3].
Oxythiamine chloride hydrochloride (0–20 μM) inhibits the invasion and migration (IC50: 8.75 μM) of Lewis lung carcinoma (LLC) cells[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Oxythiamine chloride hydrochloride Related Antibodies

Cell Viability Assay[1]

Cell Line: MIA PaCa-2 cells
Concentration: 0-40 μM
Incubation Time: 2 days
Result: Inhibited cell viability with an IC50 of 14.95 μM.

Western Blot Analysis[1]

Cell Line: MIA PaCa-2 cells
Concentration: 0, 5, 50, 500 μM
Incubation Time: 48 h
Result: Inhibited 14-3-3 protein beta/alpha expression, and increased alpha-enolase.
In Vivo

Oxythiamine chloride hydrochloride (100-500 mg/kg, i.p. 4 days) inhibits tumor growth in Ehrlich's ascites tumor hosting mice[2].
Oxythiamine chloride hydrochloride (250 or 500 mg/kg, daily for 5 week) inhibits tumor cell metastasis via inhibition of MMPs in mice implanted (s.c.) with LLC cells[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Ehrlich's ascites tumor hosting mice[2]
Dosage: 100-500 mg/kg
Administration: i.p., 4 days
Result: Inhibited tumor growth by 43% at 300 mg/kg and 84% at 500 mg/kg.
Molecular Weight

338.25

Formula

C12H17Cl2N3O2S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1=C(CCO)SC=[N+]1CC2=CN=C(C)NC2=O.Cl.[Cl-]
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (147.82 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9564 mL 14.7820 mL 29.5639 mL
5 mM 0.5913 mL 2.9564 mL 5.9128 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (6.15 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9564 mL 14.7820 mL 29.5639 mL 73.9098 mL
5 mM 0.5913 mL 2.9564 mL 5.9128 mL 14.7820 mL
10 mM 0.2956 mL 1.4782 mL 2.9564 mL 7.3910 mL
15 mM 0.1971 mL 0.9855 mL 1.9709 mL 4.9273 mL
20 mM 0.1478 mL 0.7391 mL 1.4782 mL 3.6955 mL
25 mM 0.1183 mL 0.5913 mL 1.1826 mL 2.9564 mL
30 mM 0.0985 mL 0.4927 mL 0.9855 mL 2.4637 mL
40 mM 0.0739 mL 0.3695 mL 0.7391 mL 1.8477 mL
50 mM 0.0591 mL 0.2956 mL 0.5913 mL 1.4782 mL
60 mM 0.0493 mL 0.2464 mL 0.4927 mL 1.2318 mL
80 mM 0.0370 mL 0.1848 mL 0.3695 mL 0.9239 mL
100 mM 0.0296 mL 0.1478 mL 0.2956 mL 0.7391 mL
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Oxythiamine chloride hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Oxythiamine chloride614-05-1Hydroxythiamine chlorideApoptosisEndogenous MetaboliteCancerEhrlich's ascitestumorLLCA549lung cancerInhibitorinhibitorinhibit

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