p-Nitrophenyl α-D-mannopyranoside
Based on 1 Customer Validation
p-Nitrophenyl α-D-mannopyranoside (4-Nitrophenyl α-D-mannopyranoside) is a chromogenic glycosidic ligand. p-Nitrophenyl α-D-mannopyranoside features a sugar moiety (α-D-mannopyranose) with the mannose-characteristic axial hydroxyl group at the C2 position and a ligand portion (p-nitrophenol) that provides ultraviolet-visible light absorption properties. p-Nitrophenyl α-D-mannopyranoside can be used to determine glycosidase activity and characterize the sugar recognition properties of lectins (such as Concanavalin A (HY-P2149)).
For research use only. We do not sell to patients.
- Purity: 98.88%
- CAS No.: 10357-27-4
- Formula: C12H15NO8
- Molecular Weight:301.25
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-15927
4-Nitrophenyl β-D-glucopyranoside
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HY-W011411
4-Nitrophenyl a-D-glucopyranoside
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HY-137550
4-Nitrophenyl β-D-galactopyranoside
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HY-134434
Z-Arg-Arg-AMC hydrochloride
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HY-W039892
4-Nitrophenyl β-D-glucuronide
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HY-W039911
4-Nitrophenyl α-D-galactopyranoside
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HY-W039938
4-Nitrophenyl β-D-xylopyranoside
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HY-W011183
4-Nitrophenyl-N-acetyl-β-D-glucosaminide
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HY-134420
p-Nitrophenyl α-L-fucoside
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HY-137823
4-Nitrophenyl-N-acetyl-β-D-galactosaminide
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HY-116285
n-Octyl β-D-glucopyranoside
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HY-W039897
Methyl α-D-mannopyranoside
Biological Activity
Chemical Information
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CAS No. 10357-27-4
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Appearance Solid
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Molecular Weight 301.25
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Formula C12H15NO8
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Color White to off-white
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SMILES
O[C@@H]([C@H]([C@@H]1O)O)[C@H](O[C@@H]1CO)OC(C=C2)=CC=C2[N](=O)=O
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Synonyms
4-Nitrophenyl a-D-mannopyranoside
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (331.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.30 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.30 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3195 mL | 16.5975 mL | 33.1950 mL | 82.9876 mL |
| 5 mM | 0.6639 mL | 3.3195 mL | 6.6390 mL | 16.5975 mL | |
| 10 mM | 0.3320 mL | 1.6598 mL | 3.3195 mL | 8.2988 mL | |
| 15 mM | 0.2213 mL | 1.1065 mL | 2.2130 mL | 5.5325 mL | |
| 20 mM | 0.1660 mL | 0.8299 mL | 1.6598 mL | 4.1494 mL | |
| 25 mM | 0.1328 mL | 0.6639 mL | 1.3278 mL | 3.3195 mL | |
| 30 mM | 0.1107 mL | 0.5533 mL | 1.1065 mL | 2.7663 mL | |
| 40 mM | 0.0830 mL | 0.4149 mL | 0.8299 mL | 2.0747 mL | |
| 50 mM | 0.0664 mL | 0.3320 mL | 0.6639 mL | 1.6598 mL | |
| 60 mM | 0.0553 mL | 0.2766 mL | 0.5533 mL | 1.3831 mL | |
| 80 mM | 0.0415 mL | 0.2075 mL | 0.4149 mL | 1.0373 mL | |
| 100 mM | 0.0332 mL | 0.1660 mL | 0.3320 mL | 0.8299 mL |
p-Nitrophenyl α-D-mannopyranoside Related Classifications
HY-137822 Related Classifications
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