PACAP-38 (16-38), human, mouse, rat
PACAP-38 (16-38), human, mouse, rat is a peptide fragment encompassing the C-terminal 16-38 segment of PACAP (1-38), human, ovine, rat (HY-P0221). PACAP-38 (16-38), human, mouse, rat lacks the N-terminal 1-15 receptor activation core, but retains the ability to bind to the PAC/VPAC receptor family (PAC1/VPAC1/VPAC2 receptor). PACAP-38 (16-38), human, mouse, rat promotes histamine release and exerts a certain degree of hypotensive effect.
For research use only. We do not sell to patients.
- CAS No.: 144025-82-1
- Formula: C123H215N39O28S
- Molecular Weight:2720.33
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
All PACAP Receptor Isoforms
More
Biological Activity
PACAP-38 (16-38), human, mouse, rat dose-dependently promotes histamine release in rat peritoneal mast cells[1].
PACAP-38 (16-38), human, mouse, rat (10 μM) induces concentration-dependent relaxation in phenylephrine-precontracted isolated rat caudal artery segments, but its potency is much lower than that of the full-length PACAP peptide and vasoactive intestinal polypeptide (VIP)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Sprague-Dawley (male, 250-350 g, anesthetized)[2]
-
Dosage:1.5 nmol/kg (duration measurement); cumulative doses
-
Administration:i.v.; single bolus (duration measurement); bolus with 10 min intervals
-
Result:Caused a dose-dependent reduction in systemic blood pressure.
Had a log ED50 of 3 pmol/kg.
Achieved a maximum blood pressure reduction of 43 mmHg.
Had a response duration of 150 seconds after 1.5 nmol/kg dose.
Chemical Information
-
CAS No. 144025-82-1
-
Molecular Weight 2720.33
-
Formula C123H215N39O28S
-
Sequence
Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Ala-Ala-Val-Leu-Gly-Lys-Arg-Tyr-Lys-Gln-Arg-Val-Lys-Asn-Lys-NH2
-
Sequence Shortening
QMAVKKYLAAVLGKRYKQRVKNK-NH2
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
|---|---|---|
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
|---|---|
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
Purity & Documentation
References
[1]. Mori T, et al. Histamine release induced by pituitary adenylate cyclase activating polypeptide from rat peritoneal mast cells. Arzneimittel-Forschung. 1994 Sep;44(9):1044-6. [Content Brief]
[2]. Absood A, et al. Vascular effects of pituitary adenylate cyclase activating peptide: a comparison with vasoactive intestinal peptide. Regulatory peptides. 1992 Aug 13;40(3):323-9. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PACAP-38 (16-38), human, mouse, rat Related Classifications
HY-P1817 Related Classifications
Powered by Bioz