Poly (I:C):Kanamycin (1:1) sodium
Based on 57 publication(s) in Google Scholar
Poly (I:C):Kanamycin (1:1) sodium is an isometric complex of Poly (I:C) (HY-135748) and Kanamycin (HY-16566). Poly(I:C) sodium, a synthetic analog of double-stranded RNA, is a TLR3 and retinoic acid-inducible gene I receptor (RIG-I and b>MDA5) agonist. Poly(I:C) sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses and induce apoptosis in cancer cells. Kanamycin is an orally active antibacterial agent (Gram-negative/positive bacteria) that inhibits translocation and causes miscoding by binding to the 70S ribosomal subunit. Kanamycin shows good inhibitory activity against Mycobacterium tuberculosis (susceptible and drug-resistant) and Klebsiella pneumoniae, and can be used in the research of tuberculosis and pneumonia.
For research use only. We do not sell to patients.
- Purity: 99.0%
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Poly (I:C):Kanamycin (1:1) sodium
More- Signal Transduct Target Ther. 2026 Feb 13;11(1):54. [Abstract]
- Signal Transduct Target Ther. 2025 Dec 23;10(1):410. [Abstract]
- Nature. 2025 Jul;643(8072):810-819. [Abstract]
- Imeta. 2026 Feb 24.
- Cell Metab. 2026 May 5;38(5):963-980.e12. [Abstract]
- Immunity. 2025 Sep 9;58(9):2320-2335.e9. [Abstract]
- Nat Cell Biol. 2025 Apr;27(4):683-695. [Abstract]
- ACS Nano. 2025 Feb 4;19(4):4195-4212. [Abstract]
- Hepatology. 2025 Nov 7. [Abstract]
- Nat Commun. 2025 Jun 3;16(1):5140. [Abstract]
- Nat Commun. 2025 Feb 24;16(1):1909. [Abstract]
- Acta Pharm Sin B. 2025 Oct;15(10):5431-5443. [Abstract]
- J Extracell Vesicles. 2025 May;14(5):e70083. [Abstract]
- Autophagy. 2024 May 18. [Abstract]
- Adv Sci (Weinh). 2026 Mar 18:e11012. [Abstract]
- Chem Eng J. 2021 Aug 15:418:129392. [Abstract]
- J Exp Clin Cancer Res. 2025 Feb 7;44(1):45. [Abstract]
- Mol Ther. 2025 Dec 3;33(12):6146-6159. [Abstract]
- J Exp Med. 2026 Apr 6;223(4):e20250344. [Abstract]
- Cell Commun Signal. 2026 Feb 9;24(1):168. [Abstract]
- Cancer Immunol Res. 2024 Oct 1;12(10):1438-1451. [Abstract]
- J Transl Med. 2025 Nov 10;23(1):1252. [Abstract]
- Cell Death Discov. 2025 Jul 25;11(1):345. [Abstract]
- Food Front. 2025 Nov 6.
- Clin Transl Med. 2024 Sep;14(9):e70027. [Abstract]
- Nutrients. 2025 Dec 19;18(1):21. [Abstract]
- mBio. 2026 Jun 10;17(6):e0042326. [Abstract]
- Int Immunopharmacol. 2026 May 1:176:116468. [Abstract]
- Int Immunopharmacol. 2025 Jan 11:147:114033. [Abstract]
- FASEB J. 2024 Mar 31;38(6):e23551. [Abstract]
- Virol Sin. 2023 Oct;38(5):741-754. [Abstract]
- Virol Sin. 2023 Jun;38(3):335-343. [Abstract]
- Fish Shellfish Immunol. 2026 Feb:169:111053. [Abstract]
- J Virol. 2025 Aug 19;99(8):e0049825. [Abstract]
- Cell Signal. 2025 Apr 15:132:111807. [Abstract]
- Vet Res. 2025 Nov 5;56(1):210. [Abstract]
- Vet Res. 2024 Sep 27;55(1):115. [Abstract]
- Chem Biol Drug Des. 2025 Nov;106(5):e70196. [Abstract]
- Chem Biol Drug Des. 2023 Nov;102(5):1110-1120. [Abstract]
- World J Tradit Chin Med. 2025 Oct 28.
- Cancer Med. 2023 Jul;12(14):15676-15690. [Abstract]
- J Innate Immun. 2025 Jul 28:1-27. [Abstract]
- Curr Issues Mol Biol. 2025 May 14;47(5):361. [Abstract]
- Vet Microbiol. 2025 Dec 9:313:110834. [Abstract]
- Vet Microbiol. 2025 May:304:110459. [Abstract]
- Curr Microbiol. 2024 Aug 6;81(9):296. [Abstract]
- Virology. 2026 Jun:619:110878. [Abstract]
- Dev Comp Immunol. 2025 Dec:173:105527. [Abstract]
- Dev Comp Immunol. 2025 Sep 29:172:105483. [Abstract]
- Clin Cosmet Investig Dermatol. 2024 May 14:17:1093-1105. [Abstract]
- Biochem Biophys Res Commun. 2024 Apr 16:704:149661. [Abstract]
- Transpl Immunol. 2026 Feb:94:102346. [Abstract]
- Patent. US20250235423A1.
- bioRxiv. 2024 September 23.
- bioRxiv. 2024 Dec 8:2024.12.07.627371. [Abstract]
- Res Sq. 2024 Nov 14.
- SSRN. 2023 Apr 29.
Customer Validation & Images
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RT-PCR
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WB
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IP
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RT-PCR
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RT-PCR
Biological Activity
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MDA5 |
RIG-I |
TLR3 |
Poly (I:C) sodium (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) treatment strongly reduces viability from 100% in controls to 20%–50% within 24 hours[1].
Poly (I:C) sodium (200 ng/mL; 24 hours; 1205Lu cells) treatment induces apoptosis in 1205Lu cells[1].
Poly (I:C) sodium (3 ng/mL; 24 hours; 1205Lu cells) treatment induces IFN-β expression in melanoma cells. Silencing of RIG-I and MDA-5 confirmed that induction of IFN-β by Poly (I:C) sodium required RIG-I and MDA-5, respectively, and that required IPS-1[1].
Poly (I:C) sodiumd (5 ng/mL; 24 hours; 1205Lu cells) treatment reveals active subunits of caspase-9 and caspase-8 in melanoma cells[1].
Kanamycin (0.1-100 μg/mL; 2 weeks) exhibits good antibacterial activity (MIC=1-5 μg/mL) to various strains of mycobacteria in vitro[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Kanamycin (2, 4 mg/kg; s.c.; once daily, 6 times a week for 3 weeks) inhibits growth of bovine tubercle bacilli in lung and spleen of mice[3].
Kanamycin (1.25, 5 mg/kg; s.c.; single (at 3 h after infection)) inhibits the multiplication of K. pneumonia DT-S in lung, trachea, and blood of mice and in proportion to the dose administration, and also increases the survival rate of mice[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Color White to off-white
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SMILES
[Poly (I:C):Kanamycin (1:1) (sodium)]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (57)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
2026 Feb 13;11(1):54. PMID: 41680122 -
Signal Transduct Target Ther
Oncolytic adenovirus delivery of neoantigens sensitizes low-mutation tumors to anti-PD-1 therapy and prevents metastasis. [Abstract]2025 Dec 23;10(1):410. PMID: 41429778 -
Nature
2025 Jul;643(8072):810-819. PMID: 40369065 -
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Cell Metab
Psoriasis modulates inflammatory bowel disease risk and intestinal epithelium lipid metabolism via IL-1β-producing macrophages. [Abstract]2026 May 5;38(5):963-980.e12. PMID: 41856104 -
Immunity
Spatiotemporal dynamics of CXCL10 encode contextual immune information revealed by the genetically encoded fluorescent sensor. [Abstract]2025 Sep 9;58(9):2320-2335.e9. PMID: 40818452 -
Nat Cell Biol
Selective translational control by PABPC1 phase separation regulates blast crisis and therapy resistance in chronic myeloid leukaemia. [Abstract]2025 Apr;27(4):683-695. PMID: 40102686 -
ACS Nano
2025 Feb 4;19(4):4195-4212. PMID: 39846120 -
Hepatology
GPC3-based circular RNA vaccine suppresses hepatocellular carcinoma progression by activating adaptive immune responses. [Abstract]2025 Nov 7. PMID: 41201153 -
Nat Commun
De novo design of protein condensation inhibitors by targeting an allosteric site of cGAS. [Abstract]2025 Jun 3;16(1):5140. PMID: 40461475 -
Nat Commun
Identification of splenic IRF7 as a nanotherapy target for tele-conditioning myocardial reperfusion injury. [Abstract]2025 Feb 24;16(1):1909. PMID: 39994192 -
Acta Pharm Sin B
Spatiotemporally delivery of Cas9 ribonucleoprotein/DNAzyme logic systems using near-infrared upconversion nanomachine for precise immunotherapy. [Abstract]2025 Oct;15(10):5431-5443. PMID: 41132842 -
J Extracell Vesicles
Cancer-Specific RNA Modifications in Tumour-Derived Extracellular Vesicles Promote Tumour Growth. [Abstract]2025 May;14(5):e70083. PMID: 40326665 -
Autophagy
2024 May 18. PMID: 38762760
Poly (I:C):Kanamycin (1:1) sodium purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18. [Abstract]
HEK293T cells were transfected with HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. Co-IP with HA antibodies and evaluation of LC3, TBK1 and TRIM25 with western blot using IgG as control.
Poly (I:C):Kanamycin (1:1) sodium purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18. [Abstract]
HEK293T cells were transfected with pCAGGS, HA-NSs and HA-8A, HA-PxxP, HA-DWP, or HA-WxxL at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB and IFIT1 mRNA levels were analyzed with RT-qPCR.
Poly (I:C):Kanamycin (1:1) sodium purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18. [Abstract]
HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.
Poly (I:C):Kanamycin (1:1) sodium purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18. [Abstract]
BECN1 knockout HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.
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Adv Sci (Weinh)
RNF213 Is an Interferon-Stimulated Gene That Targets Influenza A Virus NP and Activates MDA5 to Restrict Infection. [Abstract]2026 Mar 18:e11012. PMID: 41849692 -
Chem Eng J
Inhalable nanovaccine with biomimetic coronavirus structure to trigger mucosal immunity of respiratory tract against COVID-19. [Abstract]2021 Aug 15:418:129392. PMID: 33762883
Poly (I:C):Kanamycin (1:1) sodium purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2021 Aug 15:418:129392. [Abstract]
Immunofluorescence staining photographs of lung sections. Scale bars, 100 μm. (I: PBS, II: Poly(I:C), III: PL-Poly(I:C), IV: NL-Poly(I:C), V: PL-Poly(I:C)/RBD, VI: NL-Poly(I:C)/RBD).
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J Exp Clin Cancer Res
VSIG4+ tumor-associated macrophages mediate neutrophil infiltration and impair antigen-specific immunity in aggressive cancers through epigenetic regulation of SPP1. [Abstract]2025 Feb 7;44(1):45. PMID: 39920772 -
Mol Ther
Targeting O-GlcNAcylated METTL3 impedes MDS/AML progression via diminishing SRSF1 m6A modification. [Abstract]2025 Dec 3;33(12):6146-6159. PMID: 40914805 -
J Exp Med
Defects in intron recycling suppress the antiviral response via a mechanism of intronic endogenous dsRNA. [Abstract]2026 Apr 6;223(4):e20250344. PMID: 41817448 -
Cell Commun Signal
Grass carp reovirus VP35 hijacks DHX15 into phase-separated inclusion bodies to evade host antiviral immunity. [Abstract]2026 Feb 9;24(1):168. PMID: 41664180 -
Cancer Immunol Res
The E3 ubiquitin ligase FBXO38 maintains the antitumor function of natural killer cells by sustaining IL-15R signaling. [Abstract]2024 Oct 1;12(10):1438-1451. PMID: 38990095 -
J Transl Med
Truncated APC impairs innate immune response by targeting MAVS on mitochondria in colorectal cancer. [Abstract]2025 Nov 10;23(1):1252. PMID: 41214683 -
Cell Death Discov
RIPK1 S213E mutant suppresses RIPK1-dependent cell death by preventing interactions with RIPK3 and CASP8. [Abstract]2025 Jul 25;11(1):345. PMID: 40715038 -
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Clin Transl Med
IBR1, a novel endogenous IFIH1-binding dsRNA, governs IFIH1 activation and M1 macrophage polarisation in ARDS. [Abstract]2024 Sep;14(9):e70027. PMID: 39313944 -
Nutrients
Aqueous Extract of Fructus Choerospondiatis Peel Suppresses Vascular Inflammation and Alleviates Atherosclerosis via AKT/c-FOS/IL-6 Axis. [Abstract]2025 Dec 19;18(1):21. PMID: 41515139 -
mBio
Lipid droplets and small extracellular vesicles interplay in Japanese encephalitis virus non-lytic release. [Abstract]2026 Jun 10;17(6):e0042326. PMID: 42159429 -
Int Immunopharmacol
Minimolide F alleviates inflammatory diseases by specifically targeting STING and blocking IRF3 recruitment. [Abstract]2026 May 1:176:116468. PMID: 41819671 -
Int Immunopharmacol
A VZV-gE subunit vaccine decorated with mPLA elicits protective cellular immmune responses against varicella-zoster virus. [Abstract]2025 Jan 11:147:114033. PMID: 39799738 -
FASEB J
2024 Mar 31;38(6):e23551. PMID: 38489235 -
Virol Sin
RIPK3 promotes hantaviral replication by restricting JAK-STAT signaling without triggering necroptosis. [Abstract]2023 Oct;38(5):741-754. PMID: 37633447 -
Virol Sin
Antibiotic-induced gut bacteria depletion has no effect on HBV replication in HBV immune tolerance mouse model. [Abstract]2023 Jun;38(3):335-343. PMID: 37141990 -
Fish Shellfish Immunol
Cloning, functional characterization of PfLectin_C, a novel C-type lectin gene in pearl oyster Pinctada fucata, and its roles in immune defense and transplantation immunity. [Abstract]2026 Feb:169:111053. PMID: 41344455 -
J Virol
PEDV Nsp14 induces mitophagy-mediated degradation of MAVS to antagonize host innate immunity and facilitate viral proliferation. [Abstract]2025 Aug 19;99(8):e0049825. PMID: 40631894 -
Cell Signal
2025 Apr 15:132:111807. PMID: 40239727 -
Vet Res
TRIM7 suppresses transmissible gastroenteritis virus replication by targeting the degradation of N protein and activating RIG-I-mediated type I IFN antiviral response. [Abstract]2025 Nov 5;56(1):210. PMID: 41194212 -
Vet Res
2024 Sep 27;55(1):115. PMID: 39334325 -
Chem Biol Drug Des
Investigation Into the Inhibition of Ferroptosis by Praeruptorin-A in Sepsis Based on Network Pharmacology. [Abstract]2025 Nov;106(5):e70196. PMID: 41195620 -
Chem Biol Drug Des
Praeruptorin A inhibits the activation of NF-κB pathway and the expressions of inflammatory factors in poly (I:C)-induced RAW264.7 cells. [Abstract]2023 Nov;102(5):1110-1120. PMID: 37500542 -
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Cancer Med
Inhibition of FGFR3 upregulates MHC-I and PD-L1 via TLR3/NF-kB pathway in muscle-invasive bladder cancer. [Abstract]2023 Jul;12(14):15676-15690. PMID: 37283287 -
J Innate Immun
Impact of ATP Synthase Subunit β on TLR Signaling Pathway in Promoting Airway Remodeling and Heterogeneity of Small Airway Epithelial Cells in Chronic Obstructive Pulmonary Disease. [Abstract]2025 Jul 28:1-27. PMID: 40720940 -
Curr Issues Mol Biol
2025 May 14;47(5):361. PMID: 40699760 -
Vet Microbiol
miR-17195 promotes infectious bronchitis virus proliferation and macrophage-mediated inflammation via the PLCβ2-TAK1 axis. [Abstract]2025 Dec 9:313:110834. PMID: 41447967 -
Vet Microbiol
Serum amyloid P component suppresses porcine epidemic diarrhea virus replication through TLR4-mediated IFN-β signaling pathway. [Abstract]2025 May:304:110459. PMID: 40080977
Poly (I:C):Kanamycin (1:1) sodium purchased from MedChemExpress. Usage Cited in: Vet Microbiol. 2025 May:304:110459. [Abstract]
HEK-293T cells were transfected with pGFP-C1-SAP or GFP-C1 and stimulated with or without Poly (I:C):Kanamycin (1:1) and SeV, respectively. After cell collection, the expression levels of relevant factors were detected by RT-qPCR.
Poly (I:C):Kanamycin (1:1) sodium purchased from MedChemExpress. Usage Cited in: Vet Microbiol. 2025 May:304:110459. [Abstract]
HEK-293T cells were transfected with pGFP-C1-SAP or GFP-C1 and stimulated with or without Poly (I:C):Kanamycin (1:1) and SeV, respectively. After cell collection, the expression levels of relevant factors were detected by Western blotting.
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Curr Microbiol
Poly I: C Alleviated Duck Intestinal Injury Infected with Duck Viral Enteritis by Inhibiting Apoptosis. [Abstract]2024 Aug 6;81(9):296. PMID: 39105989 -
Virology
2026 Jun:619:110878. PMID: 41880901 -
Dev Comp Immunol
Genome-wide analysis unveils the differentiated "external" and "internal" lectin system in the tropical sea cucumber Holothuria leucospilota. [Abstract]2025 Dec:173:105527. PMID: 41274536 -
Dev Comp Immunol
Porcine IFI44L inhibits transmissible gastroenteritis virus (TGEV) replication by activating IFN-Ⅰ signaling pathway. [Abstract]2025 Sep 29:172:105483. PMID: 41033382 -
Clin Cosmet Investig Dermatol
Effect of Chloroquine on Type 2 Inflammatory Response in MC903-Induced Atopic Dermatitis Mice. [Abstract]2024 May 14:17:1093-1105. PMID: 38765196 -
Biochem Biophys Res Commun
Sequential administration of delta-tocotrienol ameliorates radiation-induced myelosuppression in mice and non-human primates through inducing G-CSF production. [Abstract]2024 Apr 16:704:149661. PMID: 38417343 -
Transpl Immunol
TLR3 stimulation rejuvenates aged mesenchymal stem cells and restores immunosuppressive function in graft-versus-host disease. [Abstract]2026 Feb:94:102346. PMID: 41520686 -
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bioRxiv
2024 Dec 8:2024.12.07.627371. PMID: 39677789 -
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Solvent & Solubility
H2O : 50 mg/mL (Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Robert Field, et al. Systemic challenge with the TLR3 agonist poly I:C induces amplified IFNalpha/beta and IL-1beta responses in the diseased brain and exacerbates chronic neurodegeneration. Brain Behav Immun. 2010 Aug;24(6):996-1007. [Content Brief]
[2]. Besch R, et al. Proapoptotic signaling induced by RIG-I and MDA-5 results in type I interferon-independent apoptosis in human melanoma cells. J Clin Invest. 2009 Aug;119(8):2399-411. [Content Brief]
[3]. Cheng YS, et al. Anticancer function of polyinosinic-polycytidylic acid. Cancer Biol Ther. 2010 Dec 15;10(12):1219-23. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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