2. Immunology/Inflammation Apoptosis
  3. RIG-I-like receptor (RLR) Toll-like Receptor (TLR) Apoptosis
  4. Polyinosinic-polycytidylic acid sodium

Polyinosinic-polycytidylic acid (sodium)  (Synonyms: Poly(I:C) (sodium); ポリ(I:C)(sodium))

製品番号: HY-135748 Assay: 99.4%
COA 取扱説明書 Technical Support

Polyinosinic-polycytidylic acid (Poly(I:C)) sodium is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid sodium can directly trigger cancer cells to undergo apoptosis.

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CAS 番号 : 42424-50-0

容量 価格（税別） 在庫状況 数量
5 mg $58 在庫あり
10 mg $90 在庫あり
25 mg $210 在庫あり
50 mg $360 在庫あり
100 mg $570 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

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カスタマーレビュー

Based on 62 publication(s) in Google Scholar

Other Forms of Polyinosinic-polycytidylic acid sodium:

Polyinosinic-polycytidylic acid (sodium) 関連抗体

Top Publications Citing Use of Products

顧客検証

Others
RT-PCR
WB
IP
IF

    Polyinosinic-polycytidylic acid sodium purchased from MedChemExpress. Usage Cited in: Vet Microbiol. 2025 May:304:110459.  [Abstract]

    HEK-293T cells were transfected with pGFP-C1-SAP or GFP-C1 and stimulated with or without Poly (I:C):Kanamycin (1:1) and SeV, respectively. After cell collection, the expression levels of relevant factors were detected by RT-qPCR.

    Polyinosinic-polycytidylic acid sodium purchased from MedChemExpress. Usage Cited in: Vet Microbiol. 2025 May:304:110459.  [Abstract]

    HEK-293T cells were transfected with pGFP-C1-SAP or GFP-C1 and stimulated with or without Poly (I:C):Kanamycin (1:1) and SeV, respectively. After cell collection, the expression levels of relevant factors were detected by Western blotting.

    Polyinosinic-polycytidylic acid sodium purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. Co-IP with HA antibodies and evaluation of LC3, TBK1 and TRIM25 with western blot using IgG as control.

    Polyinosinic-polycytidylic acid sodium purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with pCAGGS, HA-NSs and HA-8A, HA-PxxP, HA-DWP, or HA-WxxL at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB and IFIT1 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid sodium purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid sodium purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    BECN1 knockout HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid sodium purchased from MedChemExpress. Usage Cited in: Mol Ther Oncolytics. 2022 Aug 25:26:413-428.  [Abstract]

    Dual-luciferase reporter assay of PPAS (20 ng/ml for 24 h) on CCAR1 promoter in U251MG and 293T cells was performed.

    Polyinosinic-polycytidylic acid sodium purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2021 Aug 15:418:129392.

    Immunofluorescence staining photographs of lung sections. Scale bars, 100 μm. (I: PBS, II: Poly(I:C), III: PL-Poly(I:C), IV: NL-Poly(I:C), V: PL-Poly(I:C)/RBD, VI: NL-Poly(I:C)/RBD).

    RIG-I-like receptor (RLR) アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    RIG-I MDA5

    Toll-like Receptor (TLR) アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

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    製品説明

    Polyinosinic-polycytidylic acid (Poly(I:C)) sodium is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid sodium can directly trigger cancer cells to undergo apoptosis[1][2].

    IC50 & Target

    MDA5

     

    RIG-I

     

    TLR3

     

    体外実験

    Polyinosinic-polycytidylic acid (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) treatment strongly reduces viability from 100% in controls to 20%–50% within 24 hours[1].
    Polyinosinic-polycytidylic acid (200 ng/mL; 24 hours; 1205Lu cells) treatment induces apoptosis in 1205Lu cells[1].
    Polyinosinic-polycytidylic acid (3 ng/mL; 24 hours; 1205Lu cells) treatment induces IFN-β expression in melanoma cells. Silencing of RIG-I and MDA-5 confirmed that induction of IFN-β by Polyinosinic-polycytidylic acid required RIG-I and MDA-5, respectively, and that required IPS-1[1].
    Polyinosinic-polycytidylic acid (5 ng/mL; 24 hours; 1205Lu cells) treatment reveals active subunits of caspase-9 and caspase-8 in melanoma cells[1].
    Polyinosinic-polycytidylic acid sodium is prepared for injection by resuspending in sterile saline, heating to 50 °C at a concentration of 2 mg/mL to ensure complete solubilisation and then allowing to cool naturally to room temperature to ensure proper annealing of double-stranded RNA. Polyinosinic-polycytidylic acid is stored at -20 °C until use[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Polyinosinic-polycytidylic acid (sodium) 関連抗体

    Cell Viability Assay[1]

    Cell Line: WM793, WM278, WM239A, WM9 and 1205Lu cells
    Concentration: 20 ng/mL
    Incubation Time: 24 hours
    Result: Strongly reduced viability from 100% in controls to 20%–50% within 24 hours.

    Apoptosis Analysis[1]

    Cell Line: 1205Lu cells
    Concentration: 200 ng/mL
    Incubation Time: 24 hours
    Result: Induced apoptosis in 1205Lu cells.

    RT-PCR[1]

    Cell Line: 1205Lu cells
    Concentration: 3 ng/mL
    Incubation Time: 24 hours
    Result: Induced IFN-β expression in melanoma cells.

    Western Blot Analysis[1]

    Cell Line: 1205Lu cells
    Concentration: 5 ng/mL
    Incubation Time: 24 hours
    Result: Revealed active subunits of caspase-9 and caspase-8 in melanoma cells.
    体内実験

    Polyinosinic-polycytidylic acid treatment inhibits tumor growth in NOD/SCID immunodeficient mice injected with 1205Lu cells. The level of human DNA is 50% lower in mice treated with Polyinosinic-polycytidylic acid[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子式

    (C10H13N4O8P)x.(C9H14N3O8P)x.xNa
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@H]1[C@@H](O[C@H](COP(O)(O)=O)[C@H]1O)N2C3=C(C(NC=N3)=O)N=C2.O[C@H]4[C@H](N5C(N=C(N)C=C5)=O)O[C@H](COP(O)(O)=O)[C@H]4O.[x].[x].[Na].[x]
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    H2O : ≥ 10 mg/mL

    *"≥" means soluble, but saturation unknown.

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    一般には略語で表示されます：C1V1 = C2V2

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    体内:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: ≥ 10 mg/mL; Clear solution

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    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.4%

    COA (246 KB)

    取扱説明書 (2659 KB)
    参考文献
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    Polyinosinic-polycytidylic acid sodium Related Classifications

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Polyinosinic-polycytidylic acid42424-50-0Poly(I:C)RIG-I-like receptor (RLR)Toll-like Receptor (TLR)ApoptosisRetinoic acid-inducible gene I (RIG-I)-like receptorTLR3RIG-IMDA5immunotherapytumormicroenvironment apoptosisdsRNAproperannealingdouble-strandedRNAInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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