1. Immunology/Inflammation
    Apoptosis
  2. Toll-like Receptor (TLR)
    Apoptosis
  3. Polyinosinic-polycytidylic acid sodium

Polyinosinic-polycytidylic acid sodium (Synonyms: Poly(I:C) sodium)

Cat. No.: HY-135748 Purity: >99.0%
Handling Instructions

Polyinosinic-polycytidylic acid sodium (Poly(I:C) sodium) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid sodium can directly trigger cancer cells to undergo apoptosis.

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Polyinosinic-polycytidylic acid sodium Chemical Structure

Polyinosinic-polycytidylic acid sodium Chemical Structure

CAS No. : 42424-50-0

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Description

Polyinosinic-polycytidylic acid sodium (Poly(I:C) sodium) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid sodium can directly trigger cancer cells to undergo apoptosis[1][2].

IC50 & Target

TLR3[2], RIG-I[2], MDA5[2], apoptosis[2]

In Vitro

Polyinosinic-polycytidylic acid (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) treatment strongly reduces viability from 100% in controls to 20%–50% within 24 hours[1].
Polyinosinic-polycytidylic acid (200 ng/mL; 24 hours; 1205Lu cells) treatment induces apoptosis in 1205Lu cells[1].
Polyinosinic-polycytidylic acid (3 ng/mL; 24 hours; 1205Lu cells) treatment induces IFN-β expression in melanoma cells. Silencing of RIG-I and MDA-5 confirmed that induction of IFN-β by Polyinosinic-polycytidylic acid required RIG-I and MDA-5, respectively, and that required IPS-1[1].
Polyinosinic-polycytidylic acid (5 ng/mL; 24 hours; 1205Lu cells) treatment reveals active subunits of caspase-9 and caspase-8 in melanoma cells[1].
Polyinosinic-polycytidylic acid sodium is prepared for injection by resuspending in sterile saline, heating to 50 °C at a concentration of 2 mg/ml to ensure complete solubilisation and then allowing to cool naturally to room temperature to ensure proper annealing of double-stranded RNA. Poly I:C is stored at -20 °C until use[3].

Cell Viability Assay[1]

Cell Line: WM793, WM278, WM239A, WM9 and 1205Lu cells
Concentration: 20 ng/mL
Incubation Time: 24 hours
Result: Strongly reduced viability from 100% in controls to 20%–50% within 24 hours.

Apoptosis Analysis[1]

Cell Line: 1205Lu cells
Concentration: 200 ng/mL
Incubation Time: 24 hours
Result: Induced apoptosis in 1205Lu cells.

RT-PCR[1]

Cell Line: 1205Lu cells
Concentration: 3 ng/mL
Incubation Time: 24 hours
Result: Induced IFN-β expression in melanoma cells.

Western Blot Analysis[1]

Cell Line: 1205Lu cells
Concentration: 5 ng/mL
Incubation Time: 24 hours
Result: Revealed active subunits of caspase-9 and caspase-8 in melanoma cells.
In Vivo

Polyinosinic-polycytidylic acid treatment inhibits tumor growth in NOD/SCID immunodeficient mice injected with 1205Lu cells. The level of human DNA is 50% lower in mice treated with Polyinosinic-polycytidylic acid[1].

Formula

(C₁₀H₁₃N₄O₈P)x.(C₉H₁₄N₃O₈P)x.xNa

CAS No.

42424-50-0

SMILES

[Polyinosinic-polycytidylic acid (sodium)]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 10 mg/mL (Need ultrasonic and warming)

References

Purity: >99.0%

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Keywords:

Polyinosinic-polycytidylic acidPoly(I:C)Toll-like Receptor (TLR)ApoptosisTLR3RIG-IMDA5immunotherapytumormicroenvironment apoptosisdsRNAproperannealingdouble-strandedRNAInhibitorinhibitorinhibit

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Polyinosinic-polycytidylic acid sodium
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