ODN 1826 (Synonyms: CpG 1826)

Name: ODN 1826
Brand: MedChemExpress
SKU: HY-146245
Rating: 5.0 (10 reviews)

Cat. No.: HY-146245 Purity: 99.51%: Data Sheet
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ODN 1826 is a class B CpG ODN (oligodeoxynucleotide) and TLR9 agonist. ODN 1826 induces NO and iNOS production and enhances Apoptosis. ODN 1826 enhances immune surveillance. ODN 1826 increases aortic atherosclerotic plaque size. ODN 1826 has antitumor activity against lung cancer, glioma and melanoma.

For research use only. We do not sell to patients.

DNA, d(P-thio)(T-C-C-A-T-G-A-C-G-T-T-C-C-T-G-A-C-G-T-T)

CAS No. : 202668-42-6

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Based on 10 publication(s) in Google Scholar

Other Forms of ODN 1826:

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Biotin-labeled ODN 1826 sodium In-stock
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ODN 1826 sodium In-stock

10 Publications Citing Use of MCE ODN 1826

Cell Proliferation/Viability Assay

RT-PCR

Histological Imaging/Staining

Apoptosis Analysis

: ODN 1826 purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Sep 1:e02375. [Abstract]; ODN 1826 (CpG, 500 ng per well; 24 h) significantly promoted the repolarization of M2-typed bone marrow-derived cells (BMDM), which stood for tumor-associated macrophages (TAM), with a significant increase of M1 (iNOS) and a decrease of M2 markers (Fizz1 and Mrc1).

: ODN 1826 purchased from MedChemExpress. Usage Cited in: Life Sci. 2025 Aug 29:380:123930. [Abstract]; Cytotoxicity analysis of DC 2.4 cells treated with DNA-OprF/PcrV + CpG (ODN 1826) for 24 h. CpG was functionally integrated with the DNA vaccine via physical mixing. Specifically, 10 μg of pVAX1-OprF, 10 μg of pVAX1-PcrV, and 10 μg of CpG ODN were vortex-mixed in 150 μl of 1640 medium, followed by the addition of 60 μl of transfection reagent (mass-to-volume ratio = 1:2). The mixture was incubated at room temperature for 30 min to form transfection complexes, sterilized by passage through a 0.22 μm filter, and used immediately.

: ODN 1826 purchased from MedChemExpress. Usage Cited in: Life Sci. 2025 Aug 29:380:123930. [Abstract]; ODN 1826 (CpG, 0.1 μg/μL; s.c.; 200 μL injected every 2 weeks for a total of three times).
HE-stained image of pathological sections of the heart, liver, lung, and kidney after immunization (100×). Scale bar = 50 μm. These results revealed no obvious tissue pathological changes or inflammatory cell infiltration in the four treatment groups, including the DNA-OprF/PcrV + CpG group.

: ODN 1826 purchased from MedChemExpress. Usage Cited in: Neurochem Res. 2025 Sep 5;50(5):288. [Abstract]; ODN 1826 (CpG) regulate astrocyte cell viability and TLR9/NF-κB pathway activation. Human astrocytes were treated with varying concentrations of CpG oligodeoxynucleotides (0, 100, 1000, and 5000 ng/mL) for 24 h. Cell viability was assessed using CCK-8 assay.

: ODN 1826 purchased from MedChemExpress. Usage Cited in: Neurochem Res. 2025 Sep 5;50(5):288. [Abstract]; ODN 1826 (CpG) regulate astrocyte cell viability and TLR9/NF-κB pathway activation. Human astrocytes were treated with varying concentrations of CpG oligodeoxynucleotides (0, 100, 1000, and 5000 ng/mL) for 24 h. Apoptosis rates were determined by flow cytometry analysis using Annexin V-FITC and propidium iodide staining.

: ODN 1826 purchased from MedChemExpress. Usage Cited in: Neurochem Res. 2025 Sep 5;50(5):288. [Abstract]; ODN 1826 (CpG) regulate astrocyte cell viability and TLR9/NF-κB pathway activation. Human astrocytes were treated with varying concentrations of CpG oligodeoxynucleotides (0, 100, 1000, and 5000 ng/mL) for 24 h. Protein expression levels of TLR9, total NF-κB, and phosphorylated NF-κB (p-NF-κB) were analyzed by Western blot with GAPDH as loading control.

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nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

TLR9

In Vitro

ODN 1826 (1 μg/mL; 24 h) induces nitric oxide (NO) and inducible nitric oxide synthase (iNOS) production in RAW 264.7 mouse macrophages^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

CpG-1826 (100 μg/tumor; intratumoral injection; every 3 days for up to 4 times) delays the growth of subcutaneously implanted GL261 gliomas in C57BL/6NTac mice, inducing increased infiltration of macrophages and B cells into tumors, with significant growth delay in ~50% of mice^[4]. ODN 1826 (18 nM; s.c.; 3 times a week for 7 weeks) increases aortic atherosclerotic plaque size in a chronic vascular injury mouse model^[5].
ODN 1826 (0.05 mg; i.p.; 1, 3, 5, 8, 11, 13 days) has a strong anti-tumor growth effect in a Lewis lung cancer mouse tumor model^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	6-week-old male C57BL/6NTac mice; GL261 glioma or B16F10 melanoma subcutaneously implanted in posterior flanks^[4].
Dosage:	100 μg/tumor
Administration:	Intratumoral injection; every 3 days for up to 4 treatments
Result:	Slowed tumor growth, increased F4/80⁺ macrophages and B220⁺ B cells in tumors, with variable responses (50% mice showed prolonged stasis) (in GL261 glioma model). Achieved complete tumor regression and induced long-term immune memory in combination with Cyclophosphamide (HY-17420). Delayed growth but did not induce regression, with modest increases in F4/80⁺ macrophages and B220⁺ B cells in combination with Cyclophosphamide (in B16F10 melanoma model).

Molecular Weight

6364.10

CAS No.

202668-42-6

Appearance

Solid

Color

White to off-white

Sequence

DNA, d(P-thio)(T-C-C-A-T-G-A-C-G-T-T-C-C-T-G-A-C-G-T-T)

SMILES

[ODN 1826]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (7.86 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.1571 mL	0.7857 mL	1.5713 mL
5 mM	0.0314 mL	0.1571 mL	0.3143 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.69%

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Data Sheet (277 KB) SDS (251 KB)

COA (247 KB) LCMS (319 KB)

Handling Instructions (2242 KB)

References

[1]. Hou Y, et al. Lipoamide Ameliorates Oxidative Stress via Induction of Nrf2/ARE Signaling Pathway in PC12 Cells. J Agric Food Chem. 2019 Jul 24;67(29):8227-8234. [Content Brief]

[2]. Qi XF, et al. CpG oligodeoxynucleotide induces apoptosis and cell cycle arrest in A20 lymphoma cells via TLR9-mediated pathways. Mol Immunol. 2013 Jul;54(3-4):327-37. [Content Brief]

[3]. Utaisincharoen P, et al. CpG ODN activates NO and iNOS production in mouse macrophage cell line (RAW 264.7). Clin Exp Immunol. 2002 Jun;128(3):467-73. [Content Brief]

[4]. Jordan M, et al. CpG-1826 immunotherapy potentiates chemotherapeutic and anti-tumor immune responses to metronomic cyclophosphamide in a preclinical glioma model. Cancer Lett. 2016 Apr 1;373(1):88-96. [Content Brief]

[5]. Krogmann AO, et al. Proinflammatory Stimulation of Toll-Like Receptor 9 with High Dose CpG ODN 1826 Impairs Endothelial Regeneration and Promotes Atherosclerosis in Mice. PLoS One. 2016 Jan 11;11(1):e0146326. [Content Brief]

[6]. Yuan S, et al. CpG oligodeoxynucleotide 1826 enhances the Lewis lung cancer response to radiotherapy in murine tumor. Cancer Biother Radiopharm. 2011 Apr;26(2):203-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.1571 mL	0.7857 mL	1.5713 mL	3.9283 mL
H₂O	5 mM	0.0314 mL	0.1571 mL	0.3143 mL	0.7857 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

ODN 1826 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ODN 1826 (Synonyms: CpG 1826)

Customer Review

Other Forms of ODN 1826:

ODN 1826 Related Antibodies

10 Publications Citing Use of MCE ODN 1826

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References

Customer Review

ODN 1826 Related Antibodies

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Complete Stock Solution Preparation Table

ODN 1826 Related Classifications

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