2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. Pam3CSK4 TFA

Pam3CSK4 TFA  (Synonyms: Pam3Cys-Ser-(Lys)4 TFA)

Cat. No.: HY-P1180A Purity: 99.89%
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Pam3CSK4 TFA is a toll-like receptor 1/2 (TLR1/2) agonist with an EC50 of 0.47 ng/mL for human TLR1/2.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 112208-01-2

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 19 publication(s) in Google Scholar

Other Forms of Pam3CSK4 TFA:

Pam3CSK4 TFA Related Antibodies

Top Publications Citing Use of Products

    Pam3CSK4 TFA purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1660-1672.  [Abstract]

    Pam3CSK4 (Pam3; 1 µg/mL; 12 h). ELISA of secreted from wild-type BMDCs treated with PBS, BFP, GSDMD-NT, SK56, SK56 + GSDMD-NT, oxPAPC, SK56 + oxPAPC or pyroptotic cytomembrane fragments from mouse wild-type or Gsdmd−/− BMDMs as in c (NCF, PCF, PCF + SK56, PCFGsdmd−/−, PCFGsdmd−/− + SK56), treated or not treated with Pam3 for 12 h.

    Pam3CSK4 TFA purchased from MedChemExpress. Usage Cited in: PLoS Pathog. 2024 Nov 5;20(11):e1012614.  [Abstract]

    Pam3CSK4 (1 µg/mL; 12 h). Irg1 mRNA expression in neutrophils, pooled from three independent experiments.

    Pam3CSK4 TFA purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Jan;15(1):101054.

    Representative immunoblot images after the treatment of Oxymatrine (40 μM), Pam3CSK4 (3 μM), for five days.

    Pam3CSK4 TFA purchased from MedChemExpress. Usage Cited in: Chin Herb Med. 2024 Apr 30;16(3):422-434.  [Abstract]

    Pam3CSK4 (400 ng/mL; 4 h). Relative protein expression levels of Casp-1 p20 in BMDMs.

    Pam3CSK4 TFA purchased from MedChemExpress. Usage Cited in: Chin Herb Med. 2024 Apr 30;16(3):422-434.  [Abstract]

    Pam3CSK4 (400 ng/mL; 4 h). LPS-primed BMDMs with LJF, FF, or vehicle for 1 h and then stimulated with Pam3CSK4-primed BMDMs were pretreated with LJF, FF, or vehicle and then stimulated with transfected LPS. Activated caspase-1 (P20) and cleaved IL-1β (P17) in culture supernatants (SN) of BMDMs and NLRP3, ASC, pro-caspase-1(p45), pro-IL-1β in whole-cell lysates (WCL) by western blot analyses.

    View All Toll-like Receptor (TLR) Isoform Specific Products:

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    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Pam3CSK4 TFA is a toll-like receptor 1/2 (TLR1/2) agonist with an EC50 of 0.47 ng/mL for human TLR1/2[1].

    IC50 & Target

    TLR1

     

    TLR2

     

    In Vitro

    Pam3CSK4 is a TLR1/2 heterodimer recognises triacylated lipopeptides[1].
    Pam3CSK4 (1 μg/mL) enhances the antibacterial functions of granulocyte macrophage-colony stimulating factor (GM-CSF) induced neutrophils to Methicillin-resistant Staphylococcus aureus (MRSA)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Pam3CSK4 TFA Related Antibodies
    In Vivo

    Pam3CSK4 (5 mg/kg; i.p.; once a day for 9 days) alters brain, spleen and liver weights in neonatal mice[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Time-mated pregnant C57BL/6 wild-type mice; B6.129-Tlr2tm1Kir/J (TLR2 -deficient) mice[3]
    Dosage: 5 mg/kg
    Administration: Intraperitoneally (i.p.); once a day for 9 days
    Result: After repeated administration from postnatal day (PND3) to PND11, brain weight was decreased compared with endotoxin-free saline-treated animals at PND12. Decreased volume of cerebral gray matter, white matter in the forebrain and cerebellar molecular layer that was accompanied by an increase in spleen and liver weight at PND12.
    Molecular Weight

    1852.30

    Formula

    C87H159F9N10O19S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    Pal-Cys-Ser-Lys-Lys-Lys-Lys
    Sequence Shortening

    Pal-CSKKKK
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (13.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 16.67 mg/mL (9.00 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.5399 mL 2.6993 mL 5.3987 mL
    5 mM 0.1080 mL 0.5399 mL 1.0797 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.89%

    SDS (393 KB)

    COA (249 KB) RP-HPLC (206 KB) MS (228 KB) Elemental Analysis Report (107 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 0.5399 mL 2.6993 mL 5.3987 mL 13.4967 mL
    5 mM 0.1080 mL 0.5399 mL 1.0797 mL 2.6993 mL
    DMSO 10 mM 0.0540 mL 0.2699 mL 0.5399 mL 1.3497 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Pam3CSK4 TFA Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Pam3CSK4112208-01-2Pam3Cys-Ser-(Lys)4Toll-like Receptor (TLR)Inhibitorinhibitorinhibit

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