Cat. No.: HY-P1180

Copy Link Print Information

Pam3CSK4

(Synonyms: Pam3Cys-Ser-(Lys)4)

22 Cited Publications

Customer Review

Based on 22 publication(s) in Google Scholar

Pam3CSK4 is a toll-like receptor 1/2 (TLR1/2) agonist with an EC₅₀ of 0.47 ng/mL for human TLR1/2.

For research use only. We do not sell to patients.

CAS No.: 112208-00-1
Formula: C₈₁H₁₅₆N₁₀O₁₃S
Molecular Weight:1510.23

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Pam3CSK4 Chemical Structure

CAS No.: 112208-00-1

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (22)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Pam3CSK4 is a toll-like receptor 1/2 (TLR1/2) agonist with an EC₅₀ of 0.47 ng/mL for human TLR1/2^[1].

IC₅₀ & Target

TLR1

TLR2

All Toll-like Receptor (TLR) Isoforms :

In Vitro

Pam3CSK4 is a TLR1/2 heterodimer recognises triacylated lipopeptides^[1].
Pam3CSK4 (1 μg/mL) enhances the antibacterial functions of granulocyte macrophage-colony stimulating factor (GM-CSF) induced neutrophils to Methicillin-resistant Staphylococcus aureus^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Pam3CSK4 (5 mg/kg; i.p.; once a day for 9 days) alters brain, spleen and liver weights in neonatal mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Time-mated pregnant C57BL/6 wild-type mice; B6.129-Tlr2^tm1Kir/J (TLR2 -deficient) mice^[3]
Dosage:

5 mg/kg
Administration:

Intraperitoneally (i.p.); once a day for 9 days
Result:

After repeated administration from postnatal day (PND3) to PND11, brain weight was decreased compared with endotoxin-free saline-treated animals at PND12. Decreased volume of cerebral gray matter, white matter in the forebrain and cerebellar molecular layer that was accompanied by an increase in spleen and liver weight at PND12.

Chemical Information

CAS No. 112208-00-1
Molecular Weight 1510.23
Formula C₈₁H₁₅₆N₁₀O₁₃S
Synonyms

Pam3Cys-Ser-(Lys)4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (22)

All WBELISA

Sort by:

Journal Impact Factor

Nat Immunol

Delaying pyroptosis with an AI-screened gasdermin D pore blocker mitigates inflammatory response. [Abstract]

2025 Oct;26(10):1660-1672. PMID: 40954252

Pam3CSK4 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1660-1672. [Abstract]

Pam3CSK4 (Pam3; 1 µg/mL; 12 h). ELISA of secreted from wild-type BMDCs treated with PBS, BFP, GSDMD-NT, SK56, SK56 + GSDMD-NT, oxPAPC, SK56 + oxPAPC or pyroptotic cytomembrane fragments from mouse wild-type or Gsdmd−/− BMDMs as in c (NCF, PCF, PCF + SK56, PCF^Gsdmd−/−, PCF^Gsdmd−/− + SK56), treated or not treated with Pam3 for 12 h.
J Adv Res

4-Octyl itaconate-induced Nrf2 nuclear translocation mitigates Caspase-11-dependent noncanonical macrophage pyroptosis and acute lung injury. [Abstract]

2026 May 4:S2090-1232(26)00378-4. PMID: 42092588
Mol Biomed

Death-associated protein kinase 2 (DAPK2) propagates endoplasmic reticulum stress in macrophages to worsen sepsis through HSPA5-IRE1α axis. [Abstract]

2026 Jun 23;7(1):94. PMID: 42334722
J Control Release

Development of a lymph node-homing peptide vaccine targeting C-C chemokine receptor 7-positive dendritic cells with enhanced antitumor immunity. [Abstract]

2026 Mar 10:391:114645. PMID: 41580130
Phytomedicine

Gusongan capsule enhances osteogenic differentiation to mitigate bone loss in ovariectomized rats via the TLR2/NF-κB pathway. [Abstract]

2025 Jun:141:156705. PMID: 40215821
J Pharm Anal

Oxymatrine, a novel TLR2 agonist, promotes megakaryopoiesis and thrombopoiesis through the STING/NF-κB pathway. [Abstract]

2025 Jan;15(1):101054. PMID: 39906691

Pam3CSK4 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Jan;15(1):101054. [Abstract]

Representative immunoblot images after the treatment of Oxymatrine (40 μM), Pam3CSK4 (3 μM), for five days.
Chin Herb Med

Amplifying protection against acute lung injury: Targeting both inflammasome and cGAS-STING pathway by Lonicerae Japonicae Flos-Forsythiae Fructus drug pair. [Abstract]

2024 Apr 30;16(3):422-434. PMID: 39072201

Pam3CSK4 purchased from MedChemExpress. Usage Cited in: Chin Herb Med. 2024 Apr 30;16(3):422-434. [Abstract]

Pam3CSK4 (400 ng/mL; 4 h). Relative protein expression levels of Casp-1 p20 in BMDMs.

Pam3CSK4 purchased from MedChemExpress. Usage Cited in: Chin Herb Med. 2024 Apr 30;16(3):422-434. [Abstract]

Pam3CSK4 (400 ng/mL; 4 h). LPS-primed BMDMs with LJF, FF, or vehicle for 1 h and then stimulated with Pam3CSK4-primed BMDMs were pretreated with LJF, FF, or vehicle and then stimulated with transfected LPS. Activated caspase-1 (P20) and cleaved IL-1β (P17) in culture supernatants (SN) of BMDMs and NLRP3, ASC, pro-caspase-1(p45), pro-IL-1β in whole-cell lysates (WCL) by western blot analyses.
NPJ Sci Food

Computational-experimental study reveals direct target and bioactives of Ajania fruticulosa against NAFLD via TLR2/NF-κB/PPAR-γ signaling. [Abstract]

2026 Jan 23;10(1):73. PMID: 41577727
Sci China Life Sci

Targeting STING oligomerization with licochalcone D ameliorates STING-driven inflammatory diseases. [Abstract]

2024 Dec;67(12):2664-2677. PMID: 39190126
Food Res Int

2026 Feb 6.
Atherosclerosis

Isolinderalactone alleviates atherosclerosis by inhibiting NLRP3 inflammasome activation and inflammatory response in macrophages. [Abstract]

2026 Jan 26:414:120648. PMID: 41759391
Int Immunopharmacol

Resibufogenin, one of bufadienolides in toad venom, suppresses LPS-induced inflammation via inhibiting NF-κB and AP-1 pathways. [Abstract]

2022 Dec;113(Pt A):109312. PMID: 36252491
Int Immunopharmacol

SIX1 attenuates inflammation and rheumatoid arthritis by silencing MyD88-dependent TLR1/2 signaling. [Abstract]

2022 May:106:108613. PMID: 35180623
PLoS Pathog

Suppressing neutrophil itaconate production attenuates Mycoplasma pneumoniae pneumonia. [Abstract]

2024 Nov 5;20(11):e1012614. PMID: 39499730

Pam3CSK4 purchased from MedChemExpress. Usage Cited in: PLoS Pathog. 2024 Nov 5;20(11):e1012614. [Abstract]

Pam3CSK4 (1 µg/mL; 12 h). Irg1 mRNA expression in neutrophils, pooled from three independent experiments.
Sci Rep

Dysfunctional TLR1 reduces the therapeutic efficacy of chemotherapy by attenuating HMGB1-mediated antitumor immunity in locally advanced colorectal cancer. [Abstract]

2023 Nov 9;13(1):19440. PMID: 37945630
Sci Rep

A comparative study of spike protein of SARS-CoV-2 and its variant Omicron (B.1.1.529) on some immune characteristics. [Abstract]

2022 Oct 12;12(1):17058. PMID: 36224298
Antiviral Res

TLR2-mediated mucosal immune priming boosts anti-rhabdoviral immunity in early vertebrates. [Abstract]

2022 Jul:203:105346. PMID: 35605698
Naunyn Schmiedebergs Arch Pharmacol

Quercetin inhibits microglial pyroptosis following intracerebral hemorrhage via the TLR2/MyD88/NF-κB signaling pathway. [Abstract]

2026 Jan 19. PMID: 41549176
J Funct Foods

2026 May 30;142:107364.
Mol Immunol

Impact of knockdown LincRNA-Cox2 on apoptosis of macrophage infected with Bacillus Calmette-Guérin. [Abstract]

2021 Feb:130:85-95. PMID: 33250268
Microbes Infect

Corydalis Saxicola Bunting Total Alkaloid Eliminates Porphyromonas gingivalis strain 33277 Internalized into Macrophages by Inhibition of TLR2. [Abstract]

2024 Jan-Feb;26(1-2):105244. PMID: 37914020
Biochem Biophys Res Commun

Sequential administration of delta-tocotrienol ameliorates radiation-induced myelosuppression in mice and non-human primates through inducing G-CSF production. [Abstract]

2024 Apr 16:704:149661. PMID: 38417343

Solvent & Solubility

In Vitro

H₂O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH₄OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (262 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Irvine KL, et al. The molecular basis for recognition of bacterial ligands at equine TLR2, TLR1 and TLR6. Vet Res. 2013 Jul 4;44:50. [Content Brief]

[2]. Chen Y, et al. TLR2 agonist Pam3CSK4 enhances the antibacterial functions of GM-CSF induced neutrophils to methicillin-resistant Staphylococcus aureus. Microb Pathog. 2019 May;130:204-212. [Content Brief]

[3]. Du X, et al. Systemic stimulation of TLR2 impairs neonatal mouse brain development. PLoS One. 2011 May 6;6(5):e19583. [Content Brief]

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Pam3CSK4

Publications Citing Use of MedChemExpress (MCE) Pam3CSK4

All Toll-like Receptor (TLR) Isoforms