2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. Pam3CSK4

Pam3CSK4 is a toll-like receptor 1/2 (TLR1/2) agonist with an EC50 of 0.47 ng/mL for human TLR1/2.

At equivalent molar concentrations, both the salt and free forms of a compound exhibit comparable biological activity. Nevertheless, the salt form (Pam3CSK4 TFA) usually boasts enhanced water solubility and stability.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 112208-00-1

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Pam3CSK4 Related Antibodies

Top Publications Citing Use of Products

    Pam3CSK4 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1660-1672.  [Abstract]

    Pam3CSK4 (Pam3; 1 µg/mL; 12 h). ELISA of secreted from wild-type BMDCs treated with PBS, BFP, GSDMD-NT, SK56, SK56 + GSDMD-NT, oxPAPC, SK56 + oxPAPC or pyroptotic cytomembrane fragments from mouse wild-type or Gsdmd−/− BMDMs as in c (NCF, PCF, PCF + SK56, PCFGsdmd−/−, PCFGsdmd−/− + SK56), treated or not treated with Pam3 for 12 h.

    Pam3CSK4 purchased from MedChemExpress. Usage Cited in: PLoS Pathog. 2024 Nov 5;20(11):e1012614.  [Abstract]

    Pam3CSK4 (1 µg/mL; 12 h). Irg1 mRNA expression in neutrophils, pooled from three independent experiments.

    Pam3CSK4 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Jan;15(1):101054.

    Representative immunoblot images after the treatment of Oxymatrine (40 μM), Pam3CSK4 (3 μM), for five days.

    Pam3CSK4 purchased from MedChemExpress. Usage Cited in: Chin Herb Med. 2024 Apr 30;16(3):422-434.  [Abstract]

    Pam3CSK4 (400 ng/mL; 4 h). Relative protein expression levels of Casp-1 p20 in BMDMs.

    Pam3CSK4 purchased from MedChemExpress. Usage Cited in: Chin Herb Med. 2024 Apr 30;16(3):422-434.  [Abstract]

    Pam3CSK4 (400 ng/mL; 4 h). LPS-primed BMDMs with LJF, FF, or vehicle for 1 h and then stimulated with Pam3CSK4-primed BMDMs were pretreated with LJF, FF, or vehicle and then stimulated with transfected LPS. Activated caspase-1 (P20) and cleaved IL-1β (P17) in culture supernatants (SN) of BMDMs and NLRP3, ASC, pro-caspase-1(p45), pro-IL-1β in whole-cell lysates (WCL) by western blot analyses.

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    • Biological Activity

    • Purity & Documentation

    • References

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    Description

    Pam3CSK4 is a toll-like receptor 1/2 (TLR1/2) agonist with an EC50 of 0.47 ng/mL for human TLR1/2[1].

    IC50 & Target

    TLR1

     

    TLR2

     

    In Vitro

    Pam3CSK4 is a TLR1/2 heterodimer recognises triacylated lipopeptides[1].
    Pam3CSK4 (1 μg/mL) enhances the antibacterial functions of granulocyte macrophage-colony stimulating factor (GM-CSF) induced neutrophils to Methicillin-resistant Staphylococcus aureus[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Pam3CSK4 Related Antibodies
    In Vivo

    Pam3CSK4 (5 mg/kg; i.p.; once a day for 9 days) alters brain, spleen and liver weights in neonatal mice[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Time-mated pregnant C57BL/6 wild-type mice; B6.129-Tlr2tm1Kir/J (TLR2 -deficient) mice[3]
    Dosage: 5 mg/kg
    Administration: Intraperitoneally (i.p.); once a day for 9 days
    Result: After repeated administration from postnatal day (PND3) to PND11, brain weight was decreased compared with endotoxin-free saline-treated animals at PND12. Decreased volume of cerebral gray matter, white matter in the forebrain and cerebellar molecular layer that was accompanied by an increase in spleen and liver weight at PND12.
    Molecular Weight

    1510.23

    Formula

    C81H156N10O13S
    CAS No.
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Solvent & Solubility
    In Vitro: 

    H2O

    Peptide Solubility and Storage Guidelines:

    1.  Calculate the length of the peptide.

    2.  Calculate the overall charge of the entire peptide according to the following table:

      Contents Assign value
    Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
    Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
    Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

    3.  Recommended solution:

    Overall charge of peptide Details
    Negative (<0) 1.  Try to dissolve the peptide in water first.
    2.  If water fails, add NH4OH (<50 μL).
    3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
    Positive (>0) 1.  Try to dissolve the peptide in water first.
    2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
    3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
    Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
    2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
    Purity & Documentation
    References
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    Pam3CSK4 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Pam3CSK4112208-00-1Pam3Cys-Ser-(Lys)4Toll-like Receptor (TLR)Inhibitorinhibitorinhibit

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