2. Immunology/Inflammation Apoptosis Metabolic Enzyme/Protease Cell Cycle/DNA Damage
  3. RIG-I-like receptor (RLR) Toll-like Receptor (TLR) PKD HSP Bcl-2 Family Interleukin Related Apoptosis
  4. Poly (I:C):Kanamycin (1:1)

Poly(I:C):Kanamycin (1:1) is a mixture of Poly(I:C) (HY-107202) and Kanamycin (HY-16566). Poly(I:C) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Poly(I:C) can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Poly(I:C) can directly trigger cancer cells to undergo apoptosis. Kanamycin stabilizes Poly(I:C).

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Based on 7 publication(s) in Google Scholar

Other Forms of Poly (I:C):Kanamycin (1:1):

Poly (I:C):Kanamycin (1:1) Related Antibodies

Top Publications Citing Use of Products

    Poly (I:C):Kanamycin (1:1) purchased from MedChemExpress. Usage Cited in: Vet Microbiol. 2025 May:304:110459.  [Abstract]

    HEK-293T cells were transfected with pGFP-C1-SAP or GFP-C1 and stimulated with or without Poly (I:C):Kanamycin (1:1) and SeV, respectively. After cell collection, the expression levels of relevant factors were detected by RT-qPCR.

    Poly (I:C):Kanamycin (1:1) purchased from MedChemExpress. Usage Cited in: Vet Microbiol. 2025 May:304:110459.  [Abstract]

    HEK-293T cells were transfected with pGFP-C1-SAP or GFP-C1 and stimulated with or without Poly (I:C):Kanamycin (1:1) and SeV, respectively. After cell collection, the expression levels of relevant factors were detected by Western blotting.

    Poly (I:C):Kanamycin (1:1) purchased from MedChemExpress. Usage Cited in: Mol Ther Oncolytics. 2022 Aug 25:26:413-428.  [Abstract]

    Dual-luciferase reporter assay of PPAS (20 ng/ml for 24 h) on CCAR1 promoter in U251MG and 293T cells was performed.

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    IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

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    Description

    Poly(I:C):Kanamycin (1:1) is a mixture of Poly(I:C) (HY-107202) and Kanamycin (HY-16566). Poly(I:C) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Poly(I:C) can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Poly(I:C) can directly trigger cancer cells to undergo apoptosis. Kanamycin stabilizes Poly(I:C)[1][2].

    IC50 & Target

    MDA5

     

    RIG-I

     

    Bcl-2

     

    Bax

     

    IL-17A

     

    IL-13

     

    HSP70

     

    In Vitro

    Poly (I:C):Kanamycin (0.5-5 μg/mL, 3-24 h) induces a dose- and time-dependent increase in paracellular permeability of immortalized airway epithelial cells[4].
    Poly (I:C):Kanamycin (5 μg/mL, 24 h) does not have cytotoxicity to 16HBE14o- cells[4].
    Poly (I:C):Kanamycin (5 μg/mL, 6 h) induces disruption of epithelial apical junctional complexes (AJCs) and tight junctions (TJs) in 16HBE14o- cells[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Poly (I:C):Kanamycin (1:1) Related Antibodies

    Cell Cytotoxicity Assay[4]

    Cell Line: 16HBE14o- cells
    Concentration: 5 μg/mL
    Incubation Time: 24 h
    Result: Did not lead to significant accumulation of LDH in cell-culture medium.
    In Vivo

    Poly (I:C):Kanamycin (2.5-10 mg/mL, Stereotaxic injection, single dose) induces a sustained inflammatory reaction in the substantia nigra (SN) and in the dorsolateral striatum[2].
    Poly (I:C):Kanamycin (10 μg/mouse, Intraperitoneal injection, single dose) decreases lung tumor growth in mice[3].
    Poly (I:C):Kanamycin (1.25 mg/kg, Intraperitoneal injection, single dose) exerts therapeutic effects against cerebral I/R injury through the downregulation of TLR4/MyD88 signaling via TLR3 in MCAO model mice[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Lung tumor-bearing mice[3]
    Dosage: 10 μg/mouse
    Administration: Intraperitoneal injection (i.p.)
    Result: Induced a significant decrease in the growth of pulmonary metastases in tumor-bearing mice.
    Reduced the amount of lung foci to ≈ 40%.
    Significantly increased BAL fluid cell numbers.
    Increased the level of INF-γ and IL-17A, decreased the levels of IL-13.
    Increased TLR3 expression.
    Animal Model: Middle cerebral artery occlusion (MCAO) model mice[5]
    Dosage: 1.25 mg/kg
    Administration: Intraperitoneal injection (i.p.)
    Result: Reduced focal cerebral I/R injury.
    Increased the expression of Bcl2, Hsp27, and Hsp70, decreased Bax expression, and reduced cellular degeneration and apoptosis.
    Protected against cerebral ischemia and conferred protection against cerebral I/R injury through the downregulation of TLR4 signaling via TLR3.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    [Poly(I:C):Kanamycin(1:1)]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 50 mg/mL (Need ultrasonic)

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 20 mg/mL; Clear solution; Need ultrasonic and warming and heat to 60°C

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    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 48.60%

    SDS (251 KB)

    COA (241 KB)

    Handling Instructions (2659 KB)
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    Poly (I:C):Kanamycin (1:1) Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Poly (I:C):Kanamycin (1:1)RIG-I-like receptor (RLR)Toll-like Receptor (TLR)PKDHSPBcl-2 FamilyInterleukin RelatedApoptosisRetinoic acid-inducible gene I (RIG-I)-like receptorProtein kinase DHeat shock proteinsIL16HBE14o- cellsagonistlung tumor-bearing miceMiddle cerebral artery occlusion (MCAO) model miceInhibitorinhibitorinhibit

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