Ponceau 4R (85%)
Based on 1 Customer Validation
Ponceau 4R (85%) (Acid Red 18 (85%); New Coccine (85%)) is an orally active synthetic food colorant and a HSA-binding aggregator. Ponceau 4R (85%) binds to HSA, inducing its partial unfolding, conformational changes and aggregation. Ponceau 4R (85%) serves as a food colorant and can be used in research on diseases including type Ⅱ diabetes, Parkinson's disease, Huntington's disease and spongiform encephalopathy.
For research use only. We do not sell to patients.
- Purity: 85.0%
- CAS No.: 2611-82-7
- Formula: C20H11N2Na3O10S3
- Molecular Weight:604.47
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Ponceau 4R (5-30 µM) binds to a single site on human serum albumin at pH 2.0 via static fluorescence quenching, with a binding constant of 1.43 × 105 M-1[1].
Ponceau 4R (80-200 µM; overnight) induces aggregation of human serum albumin at pH 2.0, as measured by increased Rayleigh light scattering at 650 nm[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Ponceau 4R causes a significant increase in nuclear DNA migration in multiple organs of mice, with no associated cytotoxicity[2].
Ponceau 4R (2000 mg/kg; p.o.; single administration) exhibits low acute oral toxicity in male *Mus musculus* (house mouse), with an LD50 greater than 2000 mg/kg bw[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:unspecified (male and female)[2]
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Dosage:205 mg/kg
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Administration:p.o.; daily
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Result:Recorded cognitive deficits in first-generation male rats at the tested dose.
Observed no consistent deficits in female rats.
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Animal Model:unspecified (male)[3]
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Dosage:2000 mg/kg bw
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Administration:p.o.; single dose
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Result:Observed no mortality following single-dose administration.
Determined median lethal dose (LD50) to be greater than 2000 mg/kg bw.
Chemical Information
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CAS No. 2611-82-7
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Appearance Solid
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Molecular Weight 604.47
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Formula C20H11N2Na3O10S3
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Color Pink to red
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SMILES
O=S(C1=C2C(/N=N/C3=C4C=CC=CC4=C(S(=O)(O[Na])=O)C=C3)=C(O)C=CC2=CC(S(=O)(O[Na])=O)=C1)(O[Na])=O
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Synonyms
Acid Red 18 (85%); New Coccine (85%)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
H2O : 100 mg/mL (165.43 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (282 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Fatima M, et al. Ponceau 4R induces aggregation in human serum albumin and morin acts as an anti-aggregating agent against dye induced aggregates. Spectrochim Acta A Mol Biomol Spectrosc. 2025 Jul 5;335:125953. [Content Brief]
[2]. Amchova P, et al. Health safety issues of synthetic food colorants. Regul Toxicol Pharmacol. 2015;73(3):914-922. [Content Brief]
[3]. EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP); Villa RE, , et al. Safety and efficacy of a feed additive consisting of ponceau 4R for its use in baits for freshwater fish (GIFAP). EFSA J. 2024 Nov 15;22(11):e9072. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.6543 mL | 8.2717 mL | 16.5434 mL | 41.3585 mL |
| 5 mM | 0.3309 mL | 1.6543 mL | 3.3087 mL | 8.2717 mL | |
| 10 mM | 0.1654 mL | 0.8272 mL | 1.6543 mL | 4.1359 mL | |
| 15 mM | 0.1103 mL | 0.5514 mL | 1.1029 mL | 2.7572 mL | |
| 20 mM | 0.0827 mL | 0.4136 mL | 0.8272 mL | 2.0679 mL | |
| 25 mM | 0.0662 mL | 0.3309 mL | 0.6617 mL | 1.6543 mL | |
| 30 mM | 0.0551 mL | 0.2757 mL | 0.5514 mL | 1.3786 mL | |
| 40 mM | 0.0414 mL | 0.2068 mL | 0.4136 mL | 1.0340 mL | |
| 50 mM | 0.0331 mL | 0.1654 mL | 0.3309 mL | 0.8272 mL | |
| 60 mM | 0.0276 mL | 0.1379 mL | 0.2757 mL | 0.6893 mL | |
| 80 mM | 0.0207 mL | 0.1034 mL | 0.2068 mL | 0.5170 mL | |
| 100 mM | 0.0165 mL | 0.0827 mL | 0.1654 mL | 0.4136 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Ponceau 4R (85%) Related Classifications
HY-D0193A Related Classifications
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- Ponceau 4R (85%)
- 2611-82-7
- Acid Red 18 (85%)
- New Coccine (85%)
- Environmental Pollutants
- Drug Derivative
- polypeptide backbones
- human serum albumin
- Congo red
- domain IIA
- aromatic amino acid residues
- freshwater fish
- α-helical structure
- cross-β conformation
- mice
- rats
- food colorant
- type Ⅱ diabetes
- Parkinson's disease
- Huntington's disease
- spongiform encephalopathy
- Inhibitor
- inhibitor
- inhibit