Cat. No.: HY-N21711

Pronaphthalide A

Pronaphthalide A is a cytotoxic agent and an inhibitor of SFTSV glycoprotein Gn (domain B) (K_d = 0.74 mM). It blocks viral binding and internalization by binding to the conserved B domain of SFTSV Gn, thereby inhibiting SFTSV infection at the viral entry stage, and exhibits broad-spectrum antiviral activity against bunyaviruses. Pronaphthalide A can be used for research on gastric cancer, severe fever with thrombocytopenia syndrome, and HIV/AIDS.

For research use only. We do not sell to patients.

CAS No.: 1624617-93-1
Formula: C₂₂H₂₀O₇
Molecular Weight:396.40

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Pronaphthalide A Chemical Structure

CAS No.: 1624617-93-1

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Protocols (2)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[3]

SFTSV-Gn

0.74 mM mM (Kd)

Cellular Effect

Cell Line	Type	Value	Description	References
LoVo	IC50	0.135 μM	Cytotoxic activity against human LoVo cells assessed as reduction in cell viability incubated for 4 days by modified MTT colorimetric assay. Cytotoxic activity against human LoVo cells assessed as reduction in cell viability incubated for 4 days by modified MTT colorimetric assay.	40835052
BGC-823	IC50	0.115 μM	Cytotoxic activity against human BGC-823 cells assessed as reduction in cell viability incubated for 4 days by modified MTT colorimetric assay. Cytotoxic activity against human BGC-823 cells assessed as reduction in cell viability incubated for 4 days by modified MTT colorimetric assay.	40835052
BGC-823	IC50	0.135 μM	Cytotoxicity against human BGC-823 gastric carcinoma cells assessed as reduction in cell viability. Cytotoxicity against human BGC-823 gastric carcinoma cells assessed as reduction in cell viability.	36770856
Huh-7	IC50	0.531 μM	Anti-SFTSV activity against human Huh7 cells assessed as inhibition of viral load in cell culture supernatants measured at 48 hrs post-infection by qRT-PCR. Anti-SFTSV activity against human Huh7 cells assessed as inhibition of viral load in cell culture supernatants measured at 48 hrs post-infection by qRT-PCR.	42425227
Huh-7	CC₅₀	> 20 μM	Cytotoxicity against human Huh7 cells assessed as reduction in cell viability incubated for 48 hrs. Cytotoxicity against human Huh7 cells assessed as reduction in cell viability incubated for 48 hrs.	42425227
Huh-7	IC50	0.900 μM	Antiviral activity against LCMV in human Huh7 cells assessed as inhibition of viral replication measured at 48 hrs post-infection by qRT-PCR. Antiviral activity against LCMV in human Huh7 cells assessed as inhibition of viral replication measured at 48 hrs post-infection by qRT-PCR.	42425227
Huh-7	IC50	2.655 μM	Antiviral activity against WELV in human Huh7 cells assessed as inhibition of viral replication measured at 48 hrs post-infection by qRT-PCR. Antiviral activity against WELV in human Huh7 cells assessed as inhibition of viral replication measured at 48 hrs post-infection by qRT-PCR.	42425227

In Vitro

Pronaphthalide A (compound 1) (4 days) exhibits potent in vitro cytotoxicity against Human LoVo and BGC-823 cell lines, with IC₅₀ values of 0.135 μM and 0.115 μM, respectively^[1].
Pronaphthalide A (2.5-10 μM; 48 h) exhibits anti-SFTSV activity in Huh7 cells with an IC₅₀ of 0.531 μM and a CC₅₀ of > 20 μM^[3].
Pronaphthalide A (5-20 μM; 1 h) does not exert a direct virucidal effect on SFTSV virions^[3].
Pronaphthalide A (10 μM; from 1 h pre-infection to 24 h post-infection) inhibits SFTSV infection at the viral entry stage in Huh7 cells^[3].
Pronaphthalide A (5-20 μM; 1 h) inhibits the internalization of SFTSV in Huh7 cells^[3].
Pronaphthalide A (serially diluted concentrations; 48 h) exhibits broad-spectrum antiviral activity against LCMV (IC₅₀ = 0.900 μM) and WELV (IC₅₀ = 2.655 μM) in Huh7 cells^[3].
Pronaphthalide A (25-400 μM; 1 h) directly binds to and stabilizes SFTSV Gn in a dose-dependent manner, but does not stabilize SFTSV Gc^[3].
Pronaphthalide A (100 μM; 1 h) directly binds to SFTSV Gn, as confirmed by DARTS assay^[3].
Pronaphthalide A (1000-125 μM; 300 s) directly binds to SFTSV-Gn with a K_D of 7.40 × 10⁻⁴ M^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Real Time qPCR^[3]

View Protocol

Cell Line:

Huh7
Concentration:

2.5, 10 μM
Incubation Time:

48 h
Result:

Showed an inhibition rate exceeding 80% with cell viability maintaining above 50%.
Exhibited an IC₅₀ of 0.531 μM.
Showed a CC₅₀ of > 20 μM.
Resulted in a selection index (SI) of > 37.

Real Time qPCR^[3]

View Protocol

Cell Line:

Huh7
Concentration:

5, 10, 20 μM
Incubation Time:

1 h (37 °C); 24 hpi
Result:

Did not exhibit a virucidal effect on SFTSV.

Real Time qPCR^[3]

View Protocol

Cell Line:

Huh7
Concentration:

10 μM
Incubation Time:

1 h pre-infection; 2 h infection; 24 hpi
Result:

Significantly reduced vRNA and viral protein levels when present prior to and during infection.
Showed a more limited inhibitory effect upon post-infection addition.

Real Time qPCR^[3]

View Protocol

Cell Line:

Huh7
Concentration:

5, 10, 20 μM
Incubation Time:

1 h (37 °C); 2 h (4 °C)
Result:

Resulted in significantly lower vRNA levels compared to the control group.

Western Blot Analysis^[3]

View Protocol

Cell Line:

Huh7
Concentration:

100 μM (temperature-dependent); 25, 50, 100, 200, 400 μM (concentration-dependent)
Incubation Time:

1 h (room temperature); 5 min (heating)
Result:

Significantly enhanced the thermal stability of SFTSV Gn.
Stabilized Gn in a dose-dependent manner.
No obvious stabilization was observed for SFTSV Gc.

Western Blot Analysis^[3]

View Protocol

Cell Line:

Huh7
Concentration:

100 μM
Incubation Time:

1 h
Result:

Reduced pronase-mediated degradation of Gn.

In Vivo

Pronaphthalide A (1-5 mg/kg/day; i.p.; daily; 5 consecutive days) confers limited protection against lethal SFTSV infection in mice, achieving a maximum survival rate of 33.3% at 5 mg/kg/day with moderate viral suppression only at the high dose^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6J (male, six-week-old, anti-IFNAR1 pretreated, SFTSV HBMC16_human_2015 infected)^[3]
Dosage:

1-5 mg/kg/day
Administration:

i.p.; daily; 5 consecutive days
Result:

Achieved survival rates of 16.7% at 1 mg/kg/day and 33.3% at 5 mg/kg/day.
Moderately suppressed viral infection at the high dose.
Showed moderate inhibitory effect on viral infection in spleen sections.
Alleviated splenic pathological damage.

Chemical Information

CAS No. 1624617-93-1
Molecular Weight 396.40
Formula C₂₂H₂₀O₇
SMILES

O=C1OCC=2C(OC)=C3C=C(OC)C(OC)=CC3=C(C=4C=CC(OC)=C(O)C4)C12
Structure Classification
- Phenylpropanoids
- Lignans
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Signal Pathway Related Cell Biology

Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Jin H, et al. Cytotoxic activity of lignans from Justicia procumbens. Fitoterapia. 2014 Apr 1;94:70-6.

[2]. Carneiro MRB, et al. Overview of the Genus: Insights into Its Chemical Diversity and Biological Potential. Molecules (Basel, Switzerland). 2023 Jan 25;28(3):1190.

[3]. Huang J, et al. Effectiveness of lignans against severe fever with thrombocytopenia syndrome virus infection. Virologica Sinica. 2026 Aug;41(4):912-922.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Pronaphthalide A