Reverse transcription technology uses RNA as a template to synthesize DNA. RT-PCR is simple, specific and sensitive, and can be used to detect gene expression levels and expression differences in cells; detect RNA virus content; clone cDNA sequences of specific genes.
Molecular Clone
Molecular cloning is a genetic engineering technique used to replicate and amplify DNA or RNA sequences in vitro. Molecular cloning includes a series of molecular biology techniques including PCR (Polymerase Chain Reaction). For example, PCR can quickly and accurately copy a small segment of DNA, producing millions to billions of identical DNA molecules.
Related Experimental Schemes
Real-time quantitative PCR (qPCR) quantifies an amplifiable nucleic-acid target by monitoring fluorescence during PCR cycling rather than measuring product only after amplification. The increase in fluorescence tracks accumulation of PCR product, and the quantification cycle (Cq; historically also Ct/CP) is related to the initial amount of target: samples containing more starting target generally reach the defined fluorescence threshold in fewer cycles.
