Isolation and characterization of new epothilone analogues from recombinant Myxococcus xanthus fermentations

  • J Nat Prod. 2003 Oct;66(10):1313-7. doi: 10.1021/np030218+.
Courtney M Starks  1 ,  Yiqing Zhou ,  Fenghua Liu ,  Peter J Licari
Affiliations
  • 1. Kosan Biosciences, Inc., 3832 Bay Center Place, Hayward, California 94545, USA.
PMID: 14575429 DOI: 10.1021/np030218+
Abstract

Nine new epothilone analogues (6-8, 10, 11a, 11b, 12, 13, and 15) were isolated from fermentations of Myxococcus xanthus strains engineered with modified polyketide synthase genes. The epothilone structures were elucidated primarily through interpretation of 1D and 2D NMR data. 4-Desmethyl-10,11-didehydroepothilone D (6) displayed activity against several tumor cell lines, including a multi-drug-resistant cell line.