TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway

  • Biochem Biophys Res Commun. 2005 Aug 5;333(3):742-9. doi: 10.1016/j.bbrc.2005.05.157.
Xiujuan Chen  1 ,  Gang Lin ,  Youheng Wei ,  Saiyin Hexige ,  Yajie Niu ,  Lingling Liu ,  Chenyi Yang ,  Long Yu
Affiliations
  • 1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China.
PMID: 15964553 DOI: 10.1016/j.bbrc.2005.05.157
Abstract

Several protein kinases have been shown to be involved in spermatogenesis. Recently, a novel subfamily of serine/threonine kinases has been isolated whose expression is limited to testis. Here, we report the fifth family member, named TSSK5, which encodes a 328 amino acid protein. RT-PCR analysis showed that TSSK5 is exclusively expressed in human testis. We isolated cAMP responsive element binding protein (CREB), a TSSK5 interacting protein via yeast two-hybrid system. The in vitro kinase assay showed that TSSK5 phosphorylated CREB at Ser-133. Using a CRE reporter system, we found that TSSK5 could stimulate the CREB/CRE responsive pathway in Hek293 cells. These results suggest that this kinase may be involved in spermatogenesis through phosphorylating CREB and then stimulating the CREB/CRE responsive pathway.