The antimicrobial peptide polyphemusin localizes to the cytoplasm of Escherichia coli following treatment

  • Antimicrob Agents Chemother. 2006 Apr;50(4):1522-4. doi: 10.1128/AAC.50.4.1522-1524.2006.
Jon-Paul S Powers  1 ,  Morgan M Martin ,  Danika L Goosney ,  Robert E W Hancock
Affiliations
  • 1. Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
PMID: 16569873 DOI: 10.1128/AAC.50.4.1522-1524.2006
Abstract

The horseshoe crab peptide polyphemusin I possesses high antimicrobial activity, but its mechanism of action is as yet not well defined. Using a biotin-labeled polyphemusin I analogue and confocal fluorescence microscopy, we showed that the peptide accumulates in the cytoplasm of wild-type Escherichia coli within 30 min after addition without causing substantial membrane damage.

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