Platelet activating factor as a proinflammatory mediator in acetic-induced colitis in the rat

  • Agents Actions. 1991 Sep;34(1-2):181-4. doi: 10.1007/BF01993272.
P C Will  1 ,  T K Thomas ,  L Iverson ,  D Buckman ,  W Weis ,  C Wilson ,  A Srivastava
Affiliations
  • 1. Department of Pharmacology, Hoffmann-La Roche Inc., Nutley, NJ 07110.
PMID: 1793026 DOI: 10.1007/BF01993272
Abstract

Platelet activating factor (PAF) induces neutrophilia and produces a variety of gastrointestinal lesions. The role of PAF as a proinflammatory mediator was examined by measuring the production of PAF and the efficacy of the PAF antagonists WEB 2086 and Ro 24-0238 in acetic acid (HOAc)-induced colitis. PAF levels within the colonic mucosa, as measured by radioimmunoassay, increased from 259 +/- 119 ng/mg in control tissue, to 616 +/- 266 ng/mg in HOAc inflamed tissue. The accumulation of neutrophils within the mucosa was decreased by 53 +/- 10% by pretreatment with 3 mg/kg WEB 2086, and by 43 +/- 11% by 3 mg/kg Ro 24-0238. PAF-induced neutrophilia had no effect on the severity of HOAc-induced colitis, therefore, PAF my be involved in sustaining the chronic inflammation of colitis.

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