Effects of Ph CL 28A on eicosanoid synthesis in rat isolated hearts

  • Prostaglandins. 1991 Oct;42(4):303-12. doi: 10.1016/0090-6980(91)90079-u.
M Nakamura  1 ,  Y S Bakhle
Affiliations
  • 1. Department of Pharmacology, Hunterian Institute, Royal College of Surgeons, Lincoln's Inn Fields, London, England.
PMID: 1796146 DOI: 10.1016/0090-6980(91)90079-u
Abstract

The effects of Ph CL 28A, a derivative of sulphasalazine, on cardiac function and eicosanoid synthesis were examined in rat isolated hearts perfused via the coronary circulation. Two stimuli for eicosanoid synthesis were used: exogenous arachidonic acid (AA) or the calcium ionophore, A23187. Following exogenous AA, coronary perfusion pressure (CPP) and cardiac developed tension (CDT) increased transiently; only the CPP response was diminished by Ph CL 28A. The output of TxB2 but not that of 6-oxo-PGF1 alpha from the heart after exogenous AA was inhibited by Ph CL 28A. The ionophore, A23187, increased CPP with minor changes in CDT; Ph CL 28A did not affect either response. The ionophore released 6-oxo-PGF1 alpha, TxB2 and LTC4 from the heart but only LTC4 output was decreased by Ph CL 28A. We conclude that, although Ph CL 28A did not increase output of 6-oxo-PGF1 alpha from rat heart with either of the stimuli used, its inhibition of the output of vasoconstrictor eicosanoids could be of benefit to the coronary circulation.

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