Pyrrospirones A and B, apoptosis inducers in HL-60 cells, from an endophytic fungus, Neonectria ramulariae Wollenw KS-246

  • Bioorg Med Chem Lett. 2008 Dec 1;18(23):6050-3. doi: 10.1016/j.bmcl.2008.10.032.
Yoshihito Shiono  1 ,  Keiko Shimanuki ,  Fuminori Hiramatsu ,  Takuya Koseki ,  Murayama Tetsuya ,  Nozomi Fujisawa ,  Ken-ichi Kimura
Affiliations
  • 1. Department of Bioresource Engineering, Faculty of Agriculture, Yamagata University, Wakabamachi 1-23, Tsuruoka, Yamagata 997-8555, Japan. [email protected]
PMID: 18952424 DOI: 10.1016/j.bmcl.2008.10.032
Abstract

Pyrrospirones A and B have been isolated from unpolished rice cultures of the endophytic fungus Neonectria ramulariae Wollenw KS-246. Their absolute stereostructures (1 and 2) were elucidated through spectroscopic methods using 1D and 2D NMR techniques and chemical transformations, including the modified Mosher's method. The compounds exhibited cytotoxicity and induced Apoptosis in human promyelocytic leukemia HL-60 cells.