RNF152, a novel lysosome localized E3 ligase with pro-apoptotic activities

  • Protein Cell. 2010 Jul;1(7):656-63. doi: 10.1007/s13238-010-0083-1.
Songling Zhang  1 ,  Wei Wu ,  Yanfang Wu ,  Jiyan Zheng ,  Talin Suo ,  Hong Tang ,  Jie Tang
Affiliations
  • 1. Key Laboratory of Infection and Immunity of Chinese Academy of Sciences, the Institute of Biophysics, Beijing, 100101, China.
PMID: 21203937 DOI: 10.1007/s13238-010-0083-1
Abstract

RING finger protein 152 (RNF152) is a novel RING finger protein and has not been well characterized. We report here that RNF152 is a canonical RING finger protein and has E3 Ligase activity. It is polyubiqitinated partly through Lys-48-linked ubiquitin chains in vivo and this phenomenon is dependent on its RING finger domain and transmembrane domain. RNF152 is localized in lysosomes and co-localized with LAMP3, a lysosome marker. Moreover, over-expression of RNF152 in Hela cells induces Apoptosis. These results suggest that RNF152 is a lysosome localized E3 Ligase with pro-apoptotic activities. It is the first E3 Ligase identified so far that is involved in lysosome-related Apoptosis.