Supramolecular complexing of membane Siglec CD22 mediated by a polyvalent heterobifunctional ligand that templates on IgM

  • Bioconjug Chem. 2011 Apr 20;22(4):546-50. doi: 10.1021/bc100579d.
Lina Cui  1 ,  Pavel I Kitov ,  Gladys C Completo ,  James C Paulson ,  David R Bundle
Affiliations
  • 1. Department of Chemistry, Alberta Ingenuity Centre for Carbohydrate Science, University of Alberta , Edmonton, Alberta, Canada.
PMID: 21405019 DOI: 10.1021/bc100579d
Abstract

We report the synthesis and in vitro evaluation of a multivalent homing device, a polymer which contains preordered pendant groups with dual specificity, a trisaccharide moiety, which is specific for the Siglec CD22, and an antibody specific hapten, nitrophenol. The device efficiently attracts antihapten IgM to the surface of human lymphoma B cells as well as to CD22-conjugated Magnetic Beads by mediating the formation of a ternary complex on the surface of the target.

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