Chemical screening of an inhibitor for gibberellin receptors based on a yeast two-hybrid system

  • Bioorg Med Chem Lett. 2013 Feb 15;23(4):1096-8. doi: 10.1016/j.bmcl.2012.12.007.
Jung-Min Yoon  1 ,  Masatoshi Nakajima ,  Kiyoshi Mashiguchi ,  Seung-Hyun Park ,  Masato Otani ,  Tadao Asami
Affiliations
  • 1. Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
PMID: 23298808 DOI: 10.1016/j.bmcl.2012.12.007
Abstract

We applied a yeast two-hybrid (Y2H) system to the high-throughput monitoring of two proteins' interaction, a receptor for Phytohormone gibberellin (GA) and its direct signal transducer DELLA. With this system, we screened inhibitors to the interaction. As a result, we discovered a chemical, 3-(2-thienylsulfonyl)pyrazine-2-carbonitrile (TSPC), and we confirmed that TSPC is an inhibitor for GA perception by in vitro and in planta evaluations.

Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-125979
    TSPC
    98.09%, Gibberellin Perception Inhibitor
    target: Phytohormone
    Research Areas: Others