Enmein-type 6,7-seco-ent-kauranoids from Isodon sculponeatus

  • J Nat Prod. 2013 Nov 22;76(11):2113-9. doi: 10.1021/np400669t.
Hua-Yi Jiang  1 ,  Wei-Guang Wang ,  Min Zhou ,  Hai-Yan Wu ,  Rui Zhan ,  Xiao-Nian Li ,  Xue Du ,  Yan Li ,  Jian-Xin Pu ,  Han-Dong Sun
Affiliations
  • 1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences , Kunming 650201, People's Republic of China.
PMID: 24219809 DOI: 10.1021/np400669t
Abstract

Fourteen enmein-type 6,7-seco-ent-kaurane Diterpenoids, seven new ones (sculponins M-S, 1-7) and seven known compounds (8-14), were isolated from the aerial parts of Isodon sculponeatus . Compound 1 is the first example of an ent-kauranoid, possessing a 11,12-epoxy group, and compounds 6 and 7 have a rare 3,6-epoxy group. The structures were established primarily by NMR and MS methods, and the absolute configurations of 1, 3, and 6 were determined by single-crystal X-ray diffraction. Compound 14 showed significant cytotoxic activity against five human tumor lines, with IC50 values ranging from 1.0 to 3.5 μM, and it also inhibited NO production in LPS-stimulated RAW264.7 cells, with an IC50 value of 2.2 μM.