Cyclic dipeptides from lactic acid bacteria inhibit proliferation of the influenza A virus

  • J Microbiol. 2013 Dec;51(6):836-43. doi: 10.1007/s12275-013-3521-y.
Min-Kyu Kwak  1 ,  Rui Liu ,  Jun-Oh Kwon ,  Min-Kyu Kim ,  Andrew HyoungJin Kim ,  Sa-Ouk Kang
Affiliations
  • 1. Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul, 151-747, Republic of Korea.
PMID: 24385362 DOI: 10.1007/s12275-013-3521-y
Abstract

We isolated Lactobacillus plantarum LBP-K10 from the traditional Korean fermented food kimchi. When organic acids were removed, the culture filtrate of this isolate showed high Antiviral activity (measured using a plaque-forming assay) against the influenza A (H3N2) virus. Two fractions that were active against influenza A virus were purified from the culture filtrate using a C18 column with high-performance liquid chromatography. These active fractions were crystallized and identified to be the cyclic dipeptides cis-cyclo (L-Leu-L-Pro) and cis-cyclo(L-Phe-L-Pro) using gas chromatography-mass spectrometry; this identification was confirmed by X-ray crystallography. These cyclic dipeptides were identified in the culture filtrate of Other lactic acid bacteria, including Lactobacillus spp., Leuconostoc spp., Weissella spp., and Lactococcus lactis.

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