Rapid determination of 1-deoxynojirimycin in Morus alba L. leaves by direct analysis in real time (DART) mass spectrometry

  • J Pharm Biomed Anal. 2015 Oct 10;114:447-54. doi: 10.1016/j.jpba.2015.06.010.
Bin Xu  1 ,  Dong-Yang Zhang  2 ,  Ze-Yu Liu  2 ,  Ying Zhang  2 ,  Li Liu  2 ,  Long Li  2 ,  Charles C Liu  3 ,  Guo-Hua Wu  4
Affiliations
  • 1. School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212018, PR China.
  • 2. College of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, 212018, PR China.
  • 3. ASPEC Technologies Limited, Room 1506, RunFenDeShangBldg A, No.60 An Li Lu, Chaoyang District, Beijing 100101, PR China.
  • 4. School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212018, PR China; College of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, 212018, PR China. Electronic address: [email protected].
PMID: 26133103 DOI: 10.1016/j.jpba.2015.06.010
Abstract

A new method based on a Direct Analysis in Real Time (DART) ionization source coupled with triple quadrupole tandem mass spectrometry has been developed for rapid qualitative and quantitative analyses of 1-deoxynojirimycin (DNJ) in mulberry leaves. Two ions produced from DNJ, [M+H](+) (m/z 164) and [M-2H+H](+) (m/z 162), are observed using DART-MS in the positive ion mode. The peak areas of the two selected ions monitoring (SIM) signals of ([M+H](+) (m/z 164) and [M-2H+H](+) (m/z 162)) are integrated to determine the peak area for quantitative analyses. A reasonable linear regression equation is obtained in the range of 1.01 to 40.50 μg/mL, with a linear coefficient (R(2)) of 0.996. The limits of detection (LOD) and quantification (LOQ) of the method are 0.25 and 0.80 μg/mL, respectively. The range of recovery is shown to be 87.73-95.61%. The results derived from the developed DART-MS method are in good agreement with those from the conventional HPLC-FLD method. By contrast, DART-MS in SIM mode is a simple, rapid and high-throughput approach for the determination of the DNJ content in mulberry leaves. The present method is advantageous for the rapid screening of mulberry leaves containing high DNJ contents.

Keywords
DART–MS; DNJ; Mulberry leaves; Rapid determination; SIM.
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