Isolation, purification, and identification of antialgal substances in green alga Ulva prolifera for antialgal activity against the common harmful red tide microalgae

  • Environ Sci Pollut Res Int. 2016 Jan;23(2):1449-59. doi: 10.1007/s11356-015-5377-7.
Ying-ying Sun  1  2 ,  Hui Wang  3 ,  Gan-lin Guo  4 ,  Yin-fang Pu  4 ,  Bin-lun Yan  4 ,  Chang-hai Wang  5
Affiliations
  • 1. Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang, 222005, China. [email protected].
  • 2. Co-innovation Center of Jiangsu Marine Bio-Industry Technology, Lianyungang, 222005, China. [email protected].
  • 3. Beijing Institute of Medical Device Testing, Beijing, 101111, People's Republic of China.
  • 4. Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang, 222005, China.
  • 5. Resources and Environment Science Institute, Nanjing Agricultural University, Nanjing, 210095, China.
PMID: 26370816 DOI: 10.1007/s11356-015-5377-7
Abstract

Ten compounds (1~10) were successfully isolated from green algae Ulva prolifera through the combination of silica gel column chromatography, Sephadex LH-20 column chromatography and repeated preparative thin-layer chromatography. These ten compounds showed antialgal activity against red tide microalgae. Among them, compounds 3, 6, and 7 showed stronger antialgal activity against red tide microalgae. Furthermore, their structure was identified on the basis of spectroscopic data. There are three glycoglycerolipids: 1-O-octadecanoic acid-3-O-β-D-galactopyranosyl glycerol (2), 1-O-palmitoyl-3-O-β-D-galactopyranosyl glycerol (4), and 1-O-palmitoyl-2-O-oleoyl-3-O-β-D-galactopyranosyl glycerol (5); two monoglycerides: glycerol monopalmitate (1), 9-hexadecenoic acid, 2,3-dihydroxypropyl ester (3); two terpenoids: loliolide (6) and lsololiolide (7); one lipid-soluble pigments: zeaxanthin (8); one sterol: cholest-5-en-3-ol (9); and one alkaloid: pyrrolopiperazine-2,5-dione (10). These compounds were isolated from U. prolifera for the first time, and compounds 2, 3, 5, and 8 were isolated from marine macroalgae for the first time.

Keywords
Antialgal substances; Identification; Isolation; Purification; Red tide microalgae; Ulva prolifera.
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