The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals
- Chem Commun (Camb). 2016 Jan 14;52(4):737-40. doi: 10.1039/c5cc07354e.
Affiliations
- 1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China. [email protected] [email protected].
PMID: 26562407
DOI: 10.1039/c5cc07354e
Abstract
We developed a novel strategy by oxidation-derivatization combined mass spectrometry analysis for the determination of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA. We reported the presence of 5-formylcytosine in RNA of mammals and found that ascorbic acid and hydroquinone can increase the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine in DNA and RNA.
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