New cytotoxic and anti-inflammatory steroids from the soft coral Klyxum flaccidum

  • Bioorg Med Chem Lett. 2016 Jul 15;26(14):3253-3257. doi: 10.1016/j.bmcl.2016.05.060.
Wan-Ru Tseng  1 ,  Chiung-Yao Huang  1 ,  Yi-Ying Tsai  1 ,  Yun-Sheng Lin  2 ,  Tsong-Long Hwang  3 ,  Jui-Hsin Su  4 ,  Ping-Jyun Sung  4 ,  Chang-Feng Dai  5 ,  Jyh-Horng Sheu  6
Affiliations
  • 1. Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
  • 2. Department of Biological Science & Technology, Mei Ho University, Pingtung 912, Taiwan.
  • 3. Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan; Research Center for Industry of Human Ecology and Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan; Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan.
  • 4. National Museum of Marine Biology & Aquarium, Pingtung 944, Taiwan.
  • 5. Institute of Oceanography, National Taiwan University, Taipei 112, Taiwan.
  • 6. Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan; Graduate Institute of Natural Products, School of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: [email protected].
PMID: 27256910 DOI: 10.1016/j.bmcl.2016.05.060
Abstract

Four new Steroids, namely klyflaccisteroids G-J (1-4) were isolated from the Formosan soft coral Klyxum flaccidum. The structures of compounds 1-4 were established by spectral data analysis (IR, MS, 1D and 2D NMR) and comparison of spectral data with those of the related known compounds. Cytotoxicity assay revealed that 4 exhibited inhibition activity against the growth of HT-29, P388 and K562 Cancer cell lines, whereas 2 showed selective cytotoxicity toward P388 cells. Compound 4 was also found to display significant anti-inflammatory activity for suppressing superoxide anion generation (O2(-)) and Elastase release.

Keywords
Anti-inflammatory activity; Cytotoxicity; Klyxum flaccidum; Soft coral; Steroid.